AB232979

Anti-PDIA5 antibody

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Rabbit Polyclonal PDIA5 antibody. Suitable for WB, IHC-P and reacts with Pig, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PDIA5 aa 350 to C-terminus.

대체 명칭 보기

PDIR, PDIA5, Protein disulfide-isomerase A5, Protein disulfide isomerase-related protein

5 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDIA5 antibody (AB232979)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDIA5 antibody (AB232979)

Formalin-fixed, paraffin-embedded human liver tissue stained for PDIA5 using ab232979 at 30 μg/ml in immunohistochemical analysis. DAB staining.

Supplier Data

Western blot - Anti-PDIA5 antibody (AB232979)

All lanes:

Western blot - Anti-PDIA5 antibody (ab232979) at 2 µg/mL

All lanes:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

Predicted band size: 60 kDa

false

Supplier Data

Western blot - Anti-PDIA5 antibody (AB232979)

All lanes:

Western blot - Anti-PDIA5 antibody (ab232979) at 2 µg/mL

All lanes:

A431 (human epidermoid carcinoma cell line) cell lysate

Predicted band size: 60 kDa

false

Supplier Data

Western blot - Anti-PDIA5 antibody (AB232979)

All lanes:

Western blot - Anti-PDIA5 antibody (ab232979) at 2 µg/mL

All lanes:

Pig liver lysate

Predicted band size: 60 kDa

false

Supplier Data

Western blot - Anti-PDIA5 antibody (AB232979)

All lanes:

Western blot - Anti-PDIA5 antibody (ab232979) at 2 µg/mL

All lanes:

Recombinant human PDIA5 protein

Predicted band size: 60 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Pig

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human PDIA5 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q14554

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

Antigen-specific affinity chromatography followed by Protein A affinity chromatography.

보관 버퍼

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The protein disulfide-isomerase A5 (PDIA5) also known as PDIR serves as an enzyme that catalyzes the formation breakage and rearrangement of disulfide bonds. It weighs approximately 58 kDa. Researchers find PDIA5 expressed in the endoplasmic reticulum especially in tissues with high secretory activity like the pancreas and liver. It participates in protein folding and maintaining cell function by ensuring proper protein structure through disulfide bond formation.

Biological function summary

PDIA5 has important roles in regulating redox homeostasis and protein quality control within the endoplasmic reticulum. It does not operate alone; it functions as part of multi-protein complexes engaging with other chaperones to assist in folding newly synthesized proteins. These interactions prevent protein aggregation and accumulation of misfolded proteins protecting cells from stress.

Pathways

PDIA5 integrates into the unfolded protein response (UPR) and endoplasmic reticulum-associated degradation (ERAD) pathways. It interfaces with other proteins like BiP/GRP78 which enhances its chaperone activity during cellular stress and EDEM1 a protein involved in recognizing misfolded glycoproteins. These pathways work together to maintain cellular homeostasis by managing protein load and degradation processes.

Research associates PDIA5 with various conditions including neurodegenerative diseases and cancer. Misfolding and aggregation of proteins linked to PDIA5 malfunction can contribute to disorders like Alzheimer's disease. Moreover PDIA5 may influence cancer progression by interacting with proteins like CALR which also participates in cell stress responses. Understanding PDIA5 functions helps in developing potential therapeutic strategies for these conditions.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

See full target information PDIA5

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Molecular therapy. Nucleic acids 35:102380 PubMed39640012

2024

A lipid nanoparticle-based oligodendrocyte-specific mRNA therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Masanori Sawamura,Kiyoshi Tachikawa,Rie Hikawa,Hisako Akiyama,Seiji Kaji,Ken Yasuda,Angel I Leu,Hyojung Hong,Rajesh Mukthavaram,Pad Chivukula,Hodaka Yamakado,Yoshio Hirabayashi,Ryosuke Takahashi,Shu-Ichi Matsuzawa

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PDIA5 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDIA5 antibody (AB232979)

Western blot - Anti-PDIA5 antibody (AB232979)

Western blot - Anti-PDIA5 antibody (AB232979)

Western blot - Anti-PDIA5 antibody (AB232979)

Western blot - Anti-PDIA5 antibody (AB232979)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise