AB251492

Anti-PDK1 + PDK2 + PDK4 antibody [EPR19727] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal PDK4 antibody. Carrier free. Suitable for IP, WB and reacts with Mouse, Rat, Human, Recombinant fragment - Mouse samples.

대체 명칭 보기

PDHK4, PDK4, Pyruvate dehydrogenase kinase isoform 4, PDHK1, PDK1, Pyruvate dehydrogenase kinase isoform 1, PDH kinase 1, PDHK2, PDK2, Pyruvate dehydrogenase kinase isoform 2, PDH kinase 2, PDKII

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Supplier Data

Immunoprecipitation - Anti-PDK1 + PDK2 + PDK4 antibody [EPR19727] - BSA and Azide free (AB251492)

This data was developed using ab208187, the same antibody clone in a different buffer formulation.

PDK1+PDK2+PDK4 was immunoprecipitated from 0.35 mg of Mouse skeletal muscle whole cell lysate with ab208187 at 1/40 dilution.

Western blot was performed from the immunoprecipitate using ab208187 at 1/500 dilution.

VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1 : Mouse skeletal muscle whole cell lysate 10μg (Input).
Lane 2 : ab208187 IP in Mouse skeletal muscle whole cell lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab208187 in Mouse skeletal muscle whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 seconds.

All lanes:

Immunoprecipitation - Anti-PDK1 + PDK2 + PDK4 antibody [EPR19727] (<a href='/ko/products/primary-antibodies/pdk1-pdk2-pdk4-antibody-epr19727-ab208187'>ab208187</a>)

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Supplier Data

Western blot - Anti-PDK1 + PDK2 + PDK4 antibody [EPR19727] - BSA and Azide free (AB251492)

This data was developed using ab208187, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : Lanes 1-4 : 10 seconds; Lanes 5-6 : 3 minutes; Lane 7 : 2 seconds.

All lanes:

Western blot - Anti-PDK1 + PDK2 + PDK4 antibody [EPR19727] (<a href='/ko/products/primary-antibodies/pdk1-pdk2-pdk4-antibody-epr19727-ab208187'>ab208187</a>) at 1/1000 dilution

Lane 1:

Mouse skeletal muscle lysate at 10 µg

Lane 2:

Mouse heart tissue lysate at 10 µg

Lane 3:

Mouse liver tissue lysate at 10 µg

Lane 4:

C2C12 (Mouse myoblast cell line) whole cell lysate at 10 µg

Lane 5:

HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 10 µg

Lane 6:

Human heart lysate at 10 µg

Lane 7:

Rat heart tissue lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 47 kDa

Observed band size: 47 kDa

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Supplier Data

Western blot - Anti-PDK1 + PDK2 + PDK4 antibody [EPR19727] - BSA and Azide free (AB251492)

This data was developed using ab208187, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Mouse PDK4, PDK1 and PDK2 recombinant proteins contain aa8-412, aa1-434 and aa1-407 respectively with a His-Tag^®.

All lanes:

Western blot - Anti-PDK1 + PDK2 + PDK4 antibody [EPR19727] (<a href='/ko/products/primary-antibodies/pdk1-pdk2-pdk4-antibody-epr19727-ab208187'>ab208187</a>) at 1/10000 dilution

Lane 1:

Mouse PDK4 recombinant protein at 0.01 µg

Lane 2:

Mouse PDK1 recombinant protein at 0.01 µg

Lane 3:

Mouse PDK2 recombinant protein at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 47 kDa

false

Exposure time: 1s

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19727

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant fragment - Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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제품 세부 정보

ab251492 is the carrier-free version of ab208187.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

Do Not Freeze

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The protein targets PDK1 PDK2 and PDK4 short for pyruvate dehydrogenase kinase 1 2 and 4 are enzymes that regulate the activity of the pyruvate dehydrogenase complex (PDC) by phosphorylation inhibiting its enzymatic function. PDK1 has a mass of approximately 49 kDa PDK2 about 46 kDa and PDK4 around 48 kDa. These kinases are expressed in various tissues with PDK2 showing expression in heart pancreas and kidney while PDK4 expression is more observed in skeletal muscle and liver. PDK1 is ubiquitously expressed.

Biological function summary

These kinases play key roles in controlling cellular metabolism by inhibiting the conversion of pyruvate to acetyl-CoA a critical step linking glycolysis to the tricarboxylic acid (TCA) cycle. Acting separately but in functionally similar ways to each other PDK1 PDK2 and PDK4 help regulate energy homeostasis and preserve glucose during starvation or limited oxygen supply. These kinases are also part of larger complexes and interact with the pyruvate dehydrogenase complex and molecules like NADH and acetyl-CoA that further modulate their activity.

Pathways

PDK1 PDK2 and PDK4 are integral to the metabolic pathways that control glucose metabolism and TCA cycle by modulating pyruvate dehydrogenase complex activity. These kinases interact with signaling pathways in response to hormonal and nutrient signals adapting the body's energy metabolism under varying conditions. The insulin signaling pathway and hypoxia-inducible factor (HIF) pathway significantly influence their activity. Insulin signaling decreases the activity of PDK leading to an increase in pyruvate dehydrogenase activity and therefore in glucose oxidation. Meanwhile HIF pathway activation in low oxygen conditions upregulates PDKs to promote anaerobic metabolism.

The dysregulation of PDK1 PDK2 and PDK4 is linked to metabolic conditions such as diabetes and cancer. In diabetes an upregulation of PDK activity can lead to reduced glucose oxidation and increased blood glucose levels often connected to insulin resistance. PDKs suppress he activity of the pyruvate dehydrogenase complex leading to altered energy metabolism that is a hallmark in many cancers. Proteins such as insulin receptor substrate (IRS) in diabetes and hypoxia-inducible factor-1α (HIF-1α) in cancer are partners that link PDK activity to these disease states as they modulate PDK expression and activity in response to metabolic demands.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Inhibition of pyruvate dehydrogenase decreases glucose utilization and increases fat metabolism in response to prolonged fasting and starvation. Plays an important role in maintaining normal blood glucose levels under starvation, and is involved in the insulin signaling cascade. Via its regulation of pyruvate dehydrogenase activity, plays an important role in maintaining normal blood pH and in preventing the accumulation of ketone bodies under starvation. In the fed state, mediates cellular responses to glucose levels and to a high-fat diet. Regulates both fatty acid oxidation and de novo fatty acid biosynthesis. Plays a role in the generation of reactive oxygen species. Protects detached epithelial cells against anoikis. Plays a role in cell proliferation via its role in regulating carbohydrate and fatty acid metabolism.

See full target information PDK4

Additional targets

PDK1,PDK2

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PDK1 + PDK2 + PDK4 antibody [EPR19727] - BSA and Azide free

Immunoprecipitation - Anti-PDK1 + PDK2 + PDK4 antibody [EPR19727] - BSA and Azide free (AB251492)

Western blot - Anti-PDK1 + PDK2 + PDK4 antibody [EPR19727] - BSA and Azide free (AB251492)

Western blot - Anti-PDK1 + PDK2 + PDK4 antibody [EPR19727] - BSA and Azide free (AB251492)

관련 conjugated 항체와 다양한 조성의 항체 (2)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

Additional targets

Product promise