Anti-PDP2 antibody [MS41A 3C3BC10]

• WB

Unknown

Western blot - Anti-PDP2 antibody [MS41A 3C3BC10] (AB133982)

Predicted mature band size : 52 kDa
Observed band size : 52 kDa

Note : The antibody recognizes both the mature (52 kDa) and the immature (60 kDa) versions of PDP2 protein. The immunogen was mature PDP2 protein, lacking the first 66 amino acids which are used as a transit peptide for mitochondrial targeting. The antibody produces a band against the endogenous protein at the mature weight in tissue homogenates, but not in cancer cells where the protein may fail to mature.

All lanes:

Western blot - Anti-PDP2 antibody [MS41A 3C3BC10] (ab133982) at 1 µg/mL

Lane 2:

Recombinant human PDP2 protein (<a href='/ko/products/unavailable/recombinant-human-pdp2-protein-ab110358'>ab110358</a>) at 0.01 µg

Lane 3:

Recombinant Human PDP1/PDP protein (His tag) (<a href='/ko/products/unavailable/recombinant-human-pdp1pdp-protein-his-tag-ab110357'>ab110357</a>) at 0.01 µg

Lane 4:

Recombinant human PDK1 protein (<a href='/ko/products/unavailable/recombinant-human-pdk1-protein-ab110359'>ab110359</a>) at 0.01 µg

Lane 5:

HHH (Human heart homogenate) at 20 µg

Lane 6:

HLH (Human liver homogenate) at 20 µg

Lane 7:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 8:

HEK293 (Human embryonic kidney 293 cell line) Whole Cell Lysate at 10 µg

Lane 9:

HepG2 (Human hepatocellular carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 10:

Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Mouse IgG – H&L – Pre-Adsorbed (HRP) at 1/10000 dilution

Predicted band size: 60 kDa

Observed band size: 60 kDa

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Exposure time: 5min