AB244628

Anti-PDZ Binding Kinase antibody [EPR2519-3] - BSA and Azide free (Capture)

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal PBK/SPK antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

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Sandwich ELISA - Anti-PDZ Binding Kinase antibody [EPR2519-3] - BSA and Azide free (Capture) (AB244628)

sELISA

Unknown

Sandwich ELISA - Anti-PDZ Binding Kinase antibody [EPR2519-3] - BSA and Azide free (Capture) (AB244628)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab184866).

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2519-3

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }

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제품 세부 정보

ab244628 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human PBK. The recommended pair for sandwich ELISA is:
Capture: ab244628, Human PBK Capture Antibody (unconjugated)
Detector: ab244888, Human PBK Detector Antibody (unconjugated)
The reference range value is 300 - 5000 pg/ml.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

PDZ Binding Kinase (PBK) also known as TOPK is a serine/threonine kinase with a molecular mass of approximately 36 kDa. It performs phosphorylation on certain proteins influencing their function localization or interaction with other proteins. PBK locates mainly in the testes placenta and various tumors but also expresses in smaller amounts in normal tissues such as the liver and brain. The kinase is cell cycle-dependent with activity peaking during mitosis where it plays a role by interacting with the mitotic spindle apparatus.

Biological function summary

PBK acts as part of cellular proliferation and differentiation processes. It can form complexes with other proteins enhancing its function and stability. The kinase significantly contributes to cell growth mainly in rapidly dividing cells. PBK influences processes like spermatogenesis in testes and placental development. PBK stabilizes certain cyclins and links with proteins such as p38 MAP kinase showing a role in stress response pathways.

Pathways

PBK integrates into the mitogen-activated protein kinase (MAPK) pathway and interacts with the tumor progression pathway. These pathways are critical for transmitting signals from the surface to the nucleus affecting cell fate decisions. PBK connects with proteins like JNK and extracellular signal-regulated kinase (ERK) displaying its importance in transmitting stress and growth signals. These interactions place PBK as a node linking growth factors and extracellular signals to cellular responses.

PBK is significantly associated with cancer development and progression such as in prostate cancer and glioma. In these conditions PBK often exhibits overexpression facilitating enhanced proliferation and survival of tumor cells. The connection to proteins like Cdc2/cyclin B1 promotes unchecked cell cycle progression often found in neoplasms. PBK's role in these disorders makes it a potential biomarker or therapeutic target in oncology.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage.

See full target information PBK

대체 명칭 보기

TOPK, PBK, Lymphokine-activated killer T-cell-originated protein kinase, Cancer/testis antigen 84, MAPKK-like protein kinase, Nori-3, PDZ-binding kinase, Spermatogenesis-related protein kinase, T-LAK cell-originated protein kinase, CT84, SPK

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PDZ Binding Kinase antibody [EPR2519-3] - BSA and Azide free (Capture)

Sandwich ELISA - Anti-PDZ Binding Kinase antibody [EPR2519-3] - BSA and Azide free (Capture) (AB244628)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Assay Kits

Sample Prep & Detection Kits

Sample Prep & Detection Kits

Primary Antibodies

Primary Antibodies

Proteins & Peptides

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

Product promise