AB237338

PE Anti-6X His tag® antibody [EPR20547]

RabMAb
Recombinant
각 태그의 뜻

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(4 제품이 사용된 논문 )

Anti-6X His tag® antibody [EPR20547] - PE conjugated (ab237338) is a rabbit recombinant monoclonal antibody detecting 6X His tag® in Flow Cytometry (Intra), ICC/IF.

- Biophysical QC for unrivalled batch-batch consistency

대체 명칭 보기

6 His epitope tag, HHHHHH epitope tag, HHHHHH tag, Hexa His tag, Polyhistidine Tag, 6x his, 6x-HIS

2 이미지

Immunocytochemistry/ Immunofluorescence - PE Anti-6X His tag® antibody [EPR20547] (AB237338)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - PE Anti-6X His tag® antibody [EPR20547] (AB237338)

Confocal image showing positive staining in HEK-293T cells transfected with 6X His tag with MYC tag® expression construct.

Flow Cytometry (Intracellular) - PE Anti-6X His tag® antibody [EPR20547] (AB237338)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - PE Anti-6X His tag® antibody [EPR20547] (AB237338)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HEK-293T cells transfected with 6X His tag with MYC tag with ab237338 at 1/500 dilution (Right panel) compared with Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (ab209478) (Left panel).

Blocking with 10% Goat serum.

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20547

Isotype

IgG

Conjugation

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

Applications

ICC/IF, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "<p></p>" } } }

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제품 세부 정보

What is this antibody validated in?
PE Anti-6X His tag® antibody [EPR20547] (ab237338) is a rabbit recombinant monoclonal antibody and is validated for use in Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF). Tagged protein detection
6X His tag® antibodies are used to visualize proteins labelled with this tag in a variety of applications.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Related products
Antibody clone EPR20547 is also available pre-conjugated to a variety of labels for your convenience – Anti-6X His tag® PE [EPR20547] (ab237338).

Other related products
We have a range of other formats of antibody clone [EPR20547] also available for your convenience: ab213204, Carrier free - ab232492, Alexa Fluor® 488 - ab237336, Alexa Fluor® 647 - ab237337, PE - ab237338, HRP - ab237339, Biotin - ab316173, APC - ab317659, FITC - ab318311

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

분주 정보

Upon delivery aliquot

보관 정보

Store in the dark

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The 6X His tag is a sequence commonly used for protein purification detection and immobilization. It consists of six histidine residues that bind to metal ions like nickel or cobalt. This makes it a highly efficient tag for affinity chromatography. His tag antibodies such as anti-His HRP facilitate the detection of His-tagged proteins in various assays. Being a small tag with a typical molecular weight of about 0.84 kDa it minimally interferes with protein function. Many proteins expressed in E. coli and other expression systems carry the 6X His tag for ease of purification and analysis.

Biological function summary

Proteins tagged with the 6X His tag often play roles in studies involving recombinant proteins. The tag itself does not alter the biological function of the target protein. It is not part of any natural protein complex but aids in the study of such complexes. Researchers use His tag antibodies like anti-His to specifically target and study these tagged proteins without cross-reactivity to other proteins.

Pathways

The incorporation of the 6X His tag into proteins allows them to be studied more efficiently in various biological pathways. It can be part of pathways like signal transduction or metabolic pathways. When tagged proteins interact with other molecules such as kinases or receptors researchers can track these interactions using the tag and anti-Histidine antibodies. This technique informs on proteins that are hard to purify due to low abundance or instability.

The 6X His tag itself does not have direct implications. However proteins of interest in disease models often carry the tag which allows researchers to purify and study them in detail. For example cancer-related proteins may be expressed with a His tag to understand their role in tumorigenesis. Similarly proteins involved in metabolic disorders could be tagged which helps in analyzing specific interactions or functions disrupted in diseases.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

See full target information 6X His tag®

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제품이 사용된 논문 (4)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Communications biology 8:1322 PubMed40885856

2025

Engineering virus-like particles for safe and versatile modeling of SARS-CoV-2 host interaction and immune escape.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Ma,Yuan Lin,Siyu Ma,Kai Chen,Yihan Lin

Molecular medicine (Cambridge, Mass.) 31:170 PubMed40325370

2025

Bacteriophage-derived endolysins restore antibiotic susceptibility in β-lactam- and macrolide-resistant Streptococcus pneumoniae infections.

Applications

Unspecified application

Species

Unspecified reactive species

Niels Vander Elst,Kristine Farmen,Lisa Knörr,Lotte Merlijn,Federico Iovino

Journal of medical virology 95:e28642 PubMed36890630

2023

Pre-existing immunity to SARS-CoV-2 associates with strong T cell responses induced by inactivated COVID-19 vaccines.

Applications

Unspecified application

Species

Unspecified reactive species

Xuan Yi,Yuhao Wang,Quanrun Li,Xiaoyi Li,Panli Zhang,Xin Fu,Shuqin Gu,Daqian Zhang,Xiaoyi Liu,Haonan Lou,Yuemei Wu,Libo Tang,Jinlin Hou,Yongyin Li

International journal of molecular sciences 24: PubMed36835603

2023

4-1BB-Based CAR T Cells Effectively Reverse Exhaustion and Enhance the Anti-Tumor Immune Response through Autocrine PD-L1 scFv Antibody.

Applications

Unspecified application

Species

Unspecified reactive species

Kang Cheng,Xiangming Feng,Zhirong Chai,Zhenzhen Wang,Zheng Liu,Zhanchao Yan,Yanming Wang,Shoutao Zhang

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PE Anti-6X His tag® antibody [EPR20547]

Immunocytochemistry/ Immunofluorescence - PE Anti-6X His tag® antibody [EPR20547] (AB237338)

Flow Cytometry (Intracellular) - PE Anti-6X His tag® antibody [EPR20547] (AB237338)

관련 conjugated 항체와 다양한 조성의 항체 (8)

주요 정보

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (4)

Product promise