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AB322528

PE Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22642-17]

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Rabbit Recombinant Monoclonal Asialoglycoprotein Receptor 1/HL-1 antibody - conjugated to PE. Suitable for Flow Cyt, ICC/IF, IHC-P and reacts with Human samples.
1 이미지
Flow Cytometry - PE Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22642-17] (AB322528)
  • Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22642-17] (AB322528)

Flow cytometric analysis of HEK-293 (human embryonic kidney epithelial cell, Left) / HepG2 (human hepatocellular carcinoma epithelial cell, Right) cells labelling Asialoglycoprotein Receptor 1/HL-1 with ab322528 at 1/5000 dilution (0.01ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (ab209478) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Negative control : HEK-293 (PMID : 34508778).
Gated on viable cells.

관련 conjugated 항체와 다양한 조성의 항체 (5)

  • Unconjugated

    Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22642-17]

  • Carrier free

    Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22642-17] - BSA and Azide free

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22642-17]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22642-17]

  • HRP

    HRP Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22642-17]

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22642-17

Isotype

IgG

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Reacts with

Human, Human

Applications

IHC-P, ICC/IF, Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Assay Kits

AB309431

Human Asialoglycoprotein Receptor 1/HL-1 ELISA kit

Sandwich ELISA - Human Asialoglycoprotein Receptor 1/HL-1 ELISA kit (AB309431)

  • 0
  • 0
Reagents, Controls & Accessories

AB7481

Normal Goat Serum

Immunohistochemistry (Frozen sections) - Normal Goat Serum (AB7481)

  • 5
  • 3
Primary Antibodies

AB216897

Alexa Fluor® 488 Anti-HNF-4-alpha antibody [EPR3648]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-HNF-4-alpha antibody [EPR3648] (AB216897)

  • 3
  • 1
Reagents, Controls & Accessories

AB166640

Normal Rabbit Serum

  • 0
  • 0
Primary Antibodies

AB270094

Alexa Fluor® 488 Anti-Rab5 antibody [EPR21801] - Early Endosome Marker

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Rab5 antibody [EPR21801] - Early Endosome Marker (AB270094)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/5000", "FlowCyt-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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제품 세부 정보

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle|Store in the dark
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The Asialoglycoprotein Receptor 1 (ASGR1) also known as ASGPR 1 or asialoglycoprotein receptor is a well-known protein with a critical role in the mechanism of recognizing and binding to asialoglycoproteins. It has a molecular mass of approximately 46 kDa. ASGR1 is expressed mostly in liver cells specifically hepatocytes. The unique characteristic of this receptor is its lectin-like nature which allows it to bind to specific sugar moieties present on desialylated glycoproteins. Through receptor-mediated endocytosis it facilitates their removal from the bloodstream.
Biological function summary

The function of ASGR1 extends significantly. It operates as part of the asialoglycoprotein receptor complex interfacing directly with the ligand proteins to mediate endocytosis. This process is essential in most mammalian systems for maintaining serum glycoprotein homeostasis. By clearing desialylated glycoproteins from circulation ASGR1 helps prevent potential adverse effects these molecules might cause like immune activation or interference with other biological functions.

Pathways

ASGR1 is integral to glycoprotein clearance pathways. It actively participates in the hepatic clearance pathway ensuring the regulation of serum glycoprotein levels. The removal of asialoglycoproteins involves interactions with several other hepatic proteins such as GalNAc transferase which can further facilitate the remodeling of glycoconjugates. Understanding these interactions is key for comprehending how the liver maintains overall metabolic balance.

ASGR1 is linked to certain liver-related conditions. Dysfunction or aberrant expression of the ASGR1 receptor can relate to elevated levels of serum glycoproteins which might be observed in liver cirrhosis. Elevated glycoproteins can exacerbate the progression of liver diseases. Furthermore ASGR1 protein interaction with viral glycoproteins has implications in hepatitis infections where it may inadvertently facilitate viral entry into hepatocytes affecting liver function and contributing to disease pathology.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Transmembrane protein predominantly expressed on hepatocytes that plays a key role in endocytosis of plasma glycoproteins that lack terminal sialic acid residues. Specifically recognizes terminal galactose and N-acetylgalactosamine residues, facilitating the clearance of desialylated glycoproteins from circulation. Plays thereby a role in a variety of physiological processes, such as removal of desialylated platelets, elimination of activated lymphocytes and maintenance of serum glycoprotein homeostasis (PubMed : 25485912). Plays a role in the removal of desialylated platelets by activating a feedback loop regulating thrombopoietin (TPO) expression via the JAK2-STAT3 signaling pathway (PubMed : 25485912). Contributes also to recognition and elimination of activated lymphocytes by hepatocytes that can act as cytotoxic effectors (PubMed : 21656538). May also play a physiological role in the regulatory network of lipid homeostasis (By similarity). Upon ligand binding, the receptor-ligand complex is internalized and trafficked to a sorting organelle, where dissociation occurs. The receptor is then recycled back to the cell surface.. (Microbial infection) Plays an essential role in ACE2-independent SARS-CoV-2 entry by acting as alternate receptor.
See full target information ASGR1

대체 명칭 보기

CLEC4H1, ASGR1, Asialoglycoprotein receptor 1, ASGP-R 1, ASGPR 1, C-type lectin domain family 4 member H1, Hepatic lectin H1, HL-1
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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