PE Anti-Cardiac Troponin T antibody [EPR20266]

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - PE Anti-Cardiac Troponin T antibody [EPR20266] (AB322129)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized L6 (rat skeletal muscle myoblast) cells labelling Troponin T with ab322129 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (ab209478) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - PE Anti-Cardiac Troponin T antibody [EPR20266] (AB322129)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized L6 (rat skeletal muscle myoblast) cells labelling Troponin T with ab322129 at 1/50 (10.0 ug/ml) dilution (Green).

Confocal image showing cytoplasmic staining in L6 cells (shown in magenta). The counterstain was observed in green. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - PE Anti-Cardiac Troponin T antibody [EPR20266] (AB322129)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HL-1 (mouse atrial muscle cell) cells labelling Troponin T with ab322129 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (ab209478) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - PE Anti-Cardiac Troponin T antibody [EPR20266] (AB322129)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HL-1 (mouse atrial muscle cell) cells labelling Troponin T with ab322129 at 1/50 (10.0 ug/ml) dilution (Green).

Confocal image showing cytoplasmic staining in HL-1 cells (shown in magenta). The counterstain was observed in green. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).