AB234280

PE Anti-CD146 antibody [P1H12]

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Anti-CD146 antibody [P1H12] conjugated to PE - suitable for flow cytometry, and reacts with human samples. Clone [P1H12] is a mouse monoclonal antibody and one of the most commonly used clones for flow cytometry.

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Flow Cytometry - PE Anti-CD146 antibody [P1H12] (AB234280)

Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-CD146 antibody [P1H12] (AB234280)

HUVEC cells. Gated on singlets.

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

P1H12

Isotype

IgG1

Conjugation

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing MCAM protein. The exact immunogen used to generate this antibody is proprietary information.

P43121

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "10 µL for 10^6 Cells", "FlowCyt-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "10 µL for 10^6 Cells", "FlowCyt-species-notes": "<p></p>" }, "Dog": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Rabbit": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Size-exclusion chromatography

Purification 관련 사항

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

보관 버퍼

pH: 7.4 Preservative: 0.0975% Sodium azide Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

Do Not Freeze|Store in the dark

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

CD146 also known as MCAM or MUC18 is a cell adhesion molecule that belongs to the immunoglobulin superfamily. With a mass of approximately 113 kDa CD146 is expressed mainly on the surface of endothelial cells but it can also be found on smooth muscle cells some T-cells and in certain cancerous tissues. This protein plays a role in cell-cell adhesion contributing to the integrity and signaling functions within tissue microenvironments.

Biological function summary

CD146 influences the migration and organization of cells within the vascular system. It serves as a component of cell adhesion complexes facilitating interactions between endothelial cells and other cell types. Additionally CD146 contributes to angiogenesis the process by which new blood vessels form from existing vasculature which is critical during tissue development and repair. This function makes it an important element in maintaining normal physiological processes.

Pathways

CD146 participates in the Wnt/β-catenin and MAPK signaling pathways which are vital in cell proliferation and differentiation. Through the Wnt/β-catenin pathway CD146 interacts with other proteins like Frizzled receptors to regulate gene transcription. In the MAPK pathway it is linked with signaling cascades that involve proteins such as ERK leading to cellular responses necessary for growth and response to external stimuli.

CD146 has been implicated in melanoma progression and cardiovascular diseases. Its overexpression in melanoma associates it with increased tumor growth and metastasis often involving interactions with other adhesion molecules like integrins. In cardiovascular diseases CD146 relates to atherosclerosis as its expression on endothelial cells can promote inflammatory responses. Understanding these connections provides insights into potential therapeutic targets for treating these conditions.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as a surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration.

See full target information MCAM

대체 명칭 보기

CD146, MUC18, MCAM, Cell surface glycoprotein MUC18, Cell surface glycoprotein P1H12, Melanoma cell adhesion molecule, Melanoma-associated antigen A32, Melanoma-associated antigen MUC18, S-endo 1 endothelial-associated antigen

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Heliyon 10:e36483 PubMed39253182

2024

Navitoclax safety, tolerability, and effect on biomarkers of senescence and neurodegeneration in aged nonhuman primates.

Applications

Unspecified application

Species

Unspecified reactive species

Edward F Greenberg,Martin J Voorbach,Alexandra Smith,David R Reuter,Yuchuan Zhuang,Ji-Quan Wang,Dustin W Wooten,Elizabeth Asque,Min Hu,Carolin Hoft,Ryan Duggan,Matthew Townsend,Karin Orsi,Karen Dalecki,Willi Amberg,Lori Duggan,Heather Knight,Joseph S Spina,Yupeng He,Kennan Marsh,Vivian Zhao,Suzanne Ybarra,Jennifer Mollon,Yuni Fang,Aparna Vasanthakumar,Susan Westmoreland,Mathias Droescher,Sjoerd J Finnema,Hana Florian

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PE Anti-CD146 antibody [P1H12]

Flow Cytometry - PE Anti-CD146 antibody [P1H12] (AB234280)

관련 conjugated 항체와 다양한 조성의 항체 (3)

주요 정보

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (1)

Product promise