AB64604

PE Anti-CD18 antibody [MEM-148]

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Anti-CD18 antibody [MEM-148] conjugated to PE - suitable for flow cytometry, and reacts with human samples. Clone [MEM-148] is a mouse monoclonal antibody cited in 4 publications.

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Flow Cytometry - PE Anti-CD18 antibody [MEM-148] (AB64604)

Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-CD18 antibody [MEM-148] (AB64604)

Separation of human neutrophil granulocytes (red-filled) from CD18 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using ab64604 (concentration in sample 5 μg/ml).

Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-CD18 antibody [MEM-148] (AB64604)

Flow cytometry surface staining pattern of human peripheral whole blood stained using ab64604 (concentration in sample 5 μg/ml).

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

MEM-148

Isotype

IgG1

Conjugation

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing ITGB2 protein. The exact immunogen used to generate this antibody is proprietary information.

P05107

Specificity

This antibody recognizes the epitope exposed on free human CD18 chains unassociated with CD11 as well as on high-affinity state of LFA-1. We have data to indicate that this antibody may not cross react with Pig. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "20 µL for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/ko/products/primary-antibodies/pe-mouse-igg1-b11-6-isotype-control-ab91357'>ab91357</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p><p>Use 20 μl reagent / 100 μl of whole blood or 10^6 cells in a suspension.</p>" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Size-exclusion chromatography

보관 버퍼

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS, 0.2% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

CD18 also known as ITGB2 is a protein with a molecular weight of approximately 95 kDa. It is part of the beta-2 integrin family and partners with different alpha subunits to form heterodimers. CD18 is primarily expressed on leukocytes including neutrophils monocytes macrophages and lymphocytes. As a surface marker CD18 plays a significant role in immune cell adhesion and migration enabling cells to respond to inflammation signals effectively.

Biological function summary

The CD18 protein functions in conjunction with associated alpha subunit partners forming a part of the wider integrin family complex. ItgB2 as it is sometimes referred to is essential for cell adhesion processes. These heterodimers facilitate binding between leukocytes and various ligands on endothelial cells which subsequently controls leukocyte transmigration through vascular walls. CD18's activities are critical in immune system responses making it a prominent focus in immunological research.

Pathways

The role of CD18 extends into inflammatory and immune response signaling pathways. It contributes critically to the leukocyte adhesion cascade. This cascade engagement involves interaction with other proteins such as ICAM-1 on endothelial surfaces. CD18 through its association in integrin-mediated signaling influences processes like phagocytosis and lymphocyte activation giving it a central place in cellular immunity.

Deficiencies or mutations affecting CD18 function correlate with disorders such as Leukocyte Adhesion Deficiency type I (LAD-I). This condition results from impaired leukocyte function due to defective integrin expression. The role of CD18 in arthritis is also significant primarily by mediating leukocyte infiltration into inflamed tissues. Its interaction with proteins like ICAM-1 becomes exceptionally relevant in these disease dynamics indicating potential therapeutic targets for modulating immune responses.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Integrin ITGAL : ITGB2 is a receptor for ICAM1, ICAM2 and ICAM3 (PubMed : 1676048, PubMed : 23775590, PubMed : 38195629). Integrin ITGAL : ITGB2 is also a receptor for the secreted form of ubiquitin-like protein ISG15; the interaction is mediated by ITGAL (PubMed : 29100055). Integrins ITGAM : ITGB2 and ITGAX : ITGB2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin ITGAX : ITGB2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin ITGAM : ITGB2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin ITGAM : ITGB2 is also a receptor for factor X. Integrin ITGAD : ITGB2 is a receptor for ICAM3 and VCAM1 (PubMed : 10438935, PubMed : 8777714, PubMed : 9841932). Contributes to natural killer cell cytotoxicity (PubMed : 15356110). Involved in leukocyte adhesion and transmigration of leukocytes including T-cells and neutrophils (PubMed : 11812992, PubMed : 28807980). Triggers neutrophil transmigration during lung injury through PTK2B/PYK2-mediated activation (PubMed : 18587400). Integrin ITGAL : ITGB2 in association with ICAM3, contributes to apoptotic neutrophil phagocytosis by macrophages (PubMed : 23775590). In association with alpha subunit ITGAM/CD11b, required for CD177-PRTN3-mediated activation of TNF primed neutrophils (PubMed : 21193407). Integrins ITGAX : ITGB2 functions as a receptor of the erythrocyte-specific adhesion molecule ICAM4 and mediates erythrophagocytosis (PubMed : 16985175). Integrins ITGAX : ITGB2 functions as a receptor of the neuron-specific adhesion molecule ICAM5 ensuring neuron cell-leukocyte adhesion (PubMed : 10741396). Integrin ITGAL : ITGB2 functions as a receptor of ICAM1 by acting as a platform at the immunological synapse to translate TCR engagement and density of the ITGAL ligand ICAM1 into graded adhesion (PubMed : 38195629). Integrin ITGAM : ITGB2/MAC-1 complex functions as a signaling receptor for the ligand receptor ICAM1, ensuring adhesion between stimulated neutrophils and stimulated endothelial cells (PubMed : 1980124). Integrin ITGAL/ITGB2 that functions as a signaling receptor of ICAM2, ensuring leukocyte cell-cell adhesion on resting cells (PubMed : 1676048).

See full target information ITGB2

대체 명칭 보기

CD18, MFI7, ITGB2, Integrin beta-2, Complement receptor C3 subunit beta

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of biomedicine & biotechnology 2010:841451 PubMed20339516

2010

Identification of an MSI-H tumor-specific cytotoxic T cell epitope generated by the (-1) frame of U79260(FTO).

Applications

Unspecified application

Species

Unspecified reactive species

Michael Linnebacher,Anne Wienck,Inga Boeck,Ernst Klar

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PE Anti-CD18 antibody [MEM-148]

Flow Cytometry - PE Anti-CD18 antibody [MEM-148] (AB64604)

Flow Cytometry - PE Anti-CD18 antibody [MEM-148] (AB64604)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Specificity

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (1)

Product promise