AB326505

PE Anti-CD1A antibody [HI149]

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Mouse Monoclonal CD1A antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Human samples.

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Flow Cytometry - PE Anti-CD1A antibody [HI149] (AB326505)

Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-CD1A antibody [HI149] (AB326505)

Flow cytometry surface staining pattern of human stimulated (GM-CSF + IL-4) peripheral blood monocytes stained using ab326505 (20 μl reagent per million cells in 100 μl of cell suspension).

Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-CD1A antibody [HI149] (AB326505)

Expression profiling on peripheral blood subsets using ab326505. Innate panel

HCDM CDMaps standardized procedures (Kuzilkova D et al. Front Immunol. 2022;13 : 827898) were used for cell isolation and surface staining of blood leukocytes, with the modification of staining protocol using cytometry test tubes.
Suspension of blood leukocytes isolated from buffy coats (2 x 10^6 cells) was added to the mixture of ab326505 (1.6 µg/ml in stained blood sample) and Monocyte Blocking Buffer, vortexed and incubated for 20 min. Next, optimized backbone antibody panel (HLDA Innate) was added to test tubes, vortexed and incubated for 20 min. The residual erythrocytes were lysed with 2 ml of 10Ч diluted EXCELLYSE Easy solution and incubated for 10 min. Finally, samples were centrifuged (670 g, 5 min.), supernatant removed and the cell pellet was resuspended in 200 µl of PBS for acquisition.

Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-CD1A antibody [HI149] (AB326505)

Flow cytometry multicolor surface staining of human stimulated (GM-CSF + IL-4) peripheral blood monocytes stained using ab326505 (20 μl reagent per million cells in 100 μl of cell suspension) and anti-human CD11c (BU15) APC antibody (10 μl reagent per million cells in 100 μl of cell suspension).

Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-CD1A antibody [HI149] (AB326505)

Separation of human CD1a positive CD11c positive dendritic cells differentiated upon monocyte stimulation (GM-CSF + IL-4) (red-filled) from CD11c negative CD1a negative events (black-dashed) in flow cytometry analysis (surface staining) of human stimulated (GM-CSF + IL-4) peripheral blood monocytes stained using ab326505 (20 μl reagent per million cells in 100 μl of cell suspension).

Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-CD1A antibody [HI149] (AB326505)

Expression profiling on peripheral blood subsets using ab326505. Adaptive panel

HCDM CDMaps standardized procedures (Kuzilkova D et al. Front Immunol. 2022;13 : 827898) were used for cell isolation and surface staining of blood leukocytes, with the modification of staining protocol using cytometry test tubes.
Suspension of blood leukocytes isolated from buffy coats (2 x 10^6 cells) was added to the mixture ofab326505 (1.6 µg/ml in stained blood sample) and Monocyte Blocking Buffer, vortexed and incubated for 20 min. Next, optimized backbone antibody panel (HLDA Adaptive) was added to test tubes, vortexed and incubated for 20 min. The residual erythrocytes were lysed with 2 ml of 10Ч diluted EXCELLYSE Easy solution and incubated for 10 min. Finally, samples were centrifuged (670 g, 5 min.), supernatant removed and the cell pellet was resuspended in 200 µl of PBS for acquisition.

Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-CD1A antibody [HI149] (AB326505)

Analysis of the antibody staining profile was performed on blood leukocytes isolated from buffy coats. HCDM CDMaps standardized procedures (Kuzilkova D et al. Front Immunol. 2022;13 : 827898) were used for cell isolation and surface staining of blood leukocytes, with the modification of staining protocol using cytometry test tubes.
ab326505 was used in concentration 1.6 µg/ml in stained blood sample (2 x 10^6 cells).

Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-CD1A antibody [HI149] (AB326505)

Separation of MOLT-4 cells stained using ab326505 (concentration in sample 4 μg/m, red-filled) from MOLT-4 cells stained using mouse IgG1 isotype control (MOPC-21) PE antibody (concentration in sample 4 μg/ml, same as CD1a PE concentration, black-dashed) in flow cytometry analysis (surface staining).

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

HI149

Isotype

IgG1

Conjugation

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

Specificity

The antibody HI149 reacts with an extracellular epitope of CD1a (T6), a 49 kDa polypeptide associated with beta2-microglobulin expressed on cortical thymocytes (strongly), Langerhans cells, dendritic cells and some T cell leukemias and lymphomas. The antibody does not react with peripheral blood T and B lymphocytes, monocytes, granulocytes, platelets and erythrocytes.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.</p>" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Size-exclusion chromatography

Purification 관련 사항

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

보관 버퍼

pH: 7.4 Preservative: 0.098% Sodium azide Constituents: PBS, 0.2% BSA

배송 시 보관 조건

Ambient - Can Ship with Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

Do Not Freeze|Store in the dark

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells.

See full target information CD1A

대체 명칭 보기

CD1a, T-cell surface glycoprotein CD1a, T-cell surface antigen T6/Leu-6, hTa1 thymocyte antigen, CD1A

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PE Anti-CD1A antibody [HI149]

Flow Cytometry - PE Anti-CD1A antibody [HI149] (AB326505)

Flow Cytometry - PE Anti-CD1A antibody [HI149] (AB326505)

Flow Cytometry - PE Anti-CD1A antibody [HI149] (AB326505)

Flow Cytometry - PE Anti-CD1A antibody [HI149] (AB326505)

Flow Cytometry - PE Anti-CD1A antibody [HI149] (AB326505)

Flow Cytometry - PE Anti-CD1A antibody [HI149] (AB326505)

Flow Cytometry - PE Anti-CD1A antibody [HI149] (AB326505)

관련 conjugated 항체와 다양한 조성의 항체 (4)

주요 정보

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Specificity

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

제품 프로토콜

타겟 정보

대체 명칭 보기

Product promise