AB25498

PE Anti-CD8 alpha antibody [53-6.7]

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(2 제품이 사용된 논문 )

Anti-CD8 alpha antibody [53-6.7] conjugated to PE - suitable for flow cytometry, FuncS, IHC-Fr, IP, and reacts with mouse samples. Clone [53-6.7] is a rat monoclonal antibody and one of the most commonly used clones for flow cytometry.

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주요 정보

Host species

Rat

Clonality

Monoclonal

Clone number

53-6.7

Isotype

IgG2a

Light chain 타입

kappa

Conjugation

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

Reacts with

Mouse

Applications

Flow Cyt, IHC-Fr, IP, FuncS

applications

Immunogen

Tissue preparation containing Cd8a protein. The exact immunogen used to generate this antibody is proprietary information.

P01731

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "FuncS" : {"fullname" : "Functional Studies", "shortname":"FuncS"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/ko/products/unavailable/rat-igg2a-kappa-monoclonal-klhg2a-1-1-pe-isotype-control-ab134674'>ab134674</a> - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.", "FuncS-species-checked": "guaranteed", "FuncS-species-dilution-info": "", "FuncS-species-notes": "(In vivo and in vitro depletion of CD8a+ cells; Fractionation of CD8a+ T cells)", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "Fix with acetone." } } }

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제품 세부 정보

This antibody blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification

보관 버퍼

pH: 7.2 Preservative: 0.09% Sodium azide Constituents: 0.87% Sodium chloride, 0.14% Sodium dihydrogen phosphate, 0.1% Gelatin

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

Do Not Freeze|Store undiluted

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

CD8 alpha also known as CD8A or CD8 protein is a glycoprotein subunit expressed on the surface of cytotoxic T lymphocytes. It has a mass of approximately 32 kDa. Found on the surface cell membrane CD8 alpha functions primarily in the immune response specifically in the recognition of antigens bound to major histocompatibility complex (MHC) class I molecules. Often scientists use CD8 antibodies for detection and CD8 IHC or immunohistochemistry for localization studies.

Biological function summary

The CD8 alpha protein plays a critical role in T-cell mediated immune responses. It forms a heterodimer with the CD8 beta chain creating the CD8 alpha-beta complex that strengthens T-cell interaction with antigen-presenting cells. CD8 alpha also helps in signaling processes that activate T cells equipping them to destroy infected or malignant cells. Researchers often study CD8 alpha peptides to understand its interactions better.

Pathways

CD8 alpha is integral to the T-cell receptor signaling pathway and the cytotoxic T lymphocyte (CTL) pathway. The T-cell receptor complex which includes the CD8 molecule transmits signals that are important for T-cell activation and function. CD8 interacts with key proteins such as the T-cell receptor (TCR) and MHC class I molecules facilitating targeted responses against pathogens. These pathways highlight CD8 alpha’s role in adaptive immunity.

CD8 alpha is most prominently associated with viral infections and cancer. Conditions like HIV and some forms of leukemia show altered CD8 function highlighting the protein's role in immune surveillance. In HIV infection for instance CD8 T cells reduce in number impairing the immune response. CD8 alpha’s connection to the immune system places it alongside other immune proteins such as CD4 and MHC molecules in the context of immune dysfunction.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule : peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs). This mechanism enables CTLs to recognize and eliminate infected cells and tumor cells. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells.

See full target information Cd8a

대체 명칭 보기

CD8a, Lyt-2, Lyt2, Cd8a, T-cell surface glycoprotein CD8 alpha chain, T-cell surface glycoprotein Lyt-2

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cell reports. Medicine 5:101590 PubMed38843844

2024

Glycoengineering-based anti-PD-1-iRGD peptide conjugate boosts antitumor efficacy through T cell engagement.

Applications

Unspecified application

Species

Unspecified reactive species

Yunfeng Pan,Qi Xue,Yi Yang,Tao Shi,Hanbing Wang,Xueru Song,Yuting Luo,Wenqi Liu,Shiji Ren,Yiran Cai,Yang Nie,Zhentao Song,Baorui Liu,Jie P Li,Jia Wei

PloS one 9:e86348 PubMed24466045

2014

CD4+ T-cell help is required for effective CD8+ T cell-mediated resolution of acute viral hepatitis in mice.

Applications

IHC-Fr

Species

Mouse

Tanja Trautmann,Jan-Hendrik Kozik,Antonella Carambia,Kirsten Richter,Timo Lischke,Dorothee Schwinge,Hans-Willi Mittrücker,Ansgar W Lohse,Annette Oxenius,Christiane Wiegard,Johannes Herkel

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PE Anti-CD8 alpha antibody [53-6.7]

관련 conjugated 항체와 다양한 조성의 항체 (9)

주요 정보

Host species

Clonality

Clone number

Isotype

Light chain 타입

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (2)

Product promise