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AB211349

PE Anti-CTP synthase/CTPS antibody [EPR8086(B)]

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Rabbit Recombinant Monoclonal CTP synthase/CTPS antibody - conjugated to PE. Suitable for Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

대체 명칭 보기

CTPS, CTPS1, CTP synthase 1, CTP synthetase 1, Protein-asparagine deamidase CTPS1, UTP--ammonia ligase 1

1 이미지
Flow Cytometry (Intracellular) - PE Anti-CTP synthase/CTPS antibody [EPR8086(B)] (AB211349)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - PE Anti-CTP synthase/CTPS antibody [EPR8086(B)] (AB211349)

Overlay histogram showing HeLa cells stained with ab211349 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab211349, 1/500 dilution) for 30 min at 22°C.

Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

관련 conjugated 항체와 다양한 조성의 항체 (2)

  • Unconjugated

    Anti-CTP synthase/CTPS antibody [EPR8086(B)]

  • Carrier free

    Anti-CTP synthase/CTPS antibody [EPR8086(B)] - BSA and Azide free

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8086(B)

Isotype

IgG

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Reacts with

Human, Human

Applications

Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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대체품

Primary Antibodies

AB244492

Anti-CTP synthase/CTPS antibody

Immunocytochemistry/ Immunofluorescence - Anti-CTP synthase/CTPS antibody (AB244492)

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Primary Antibodies

AB235000

Anti-CTP synthase/CTPS antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTP synthase/CTPS antibody (AB235000)

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보완 제품

Proteins & Peptides

AB132558

Recombinant Human CTP synthase/CTPS protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human CTP synthase/CTPS protein (GST tag N-Terminus) (AB132558)

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Assay Kits

AB234055

ATPase Assay Kit (Colorimetric)

Functional Studies - ATPase Assay Kit (Colorimetric) (AB234055)

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  • 0
Assay Kits

AB234041

Methionine Assay Kit (Fluorometric)

Functional Studies - Methionine Assay Kit (Fluorometric) (AB234041)

  • 3
  • 1
Assay Kits

AB272520

ATPase/GTPase Assay Kit

Enzyme activity assay - ATPase/GTPase Assay Kit (AB272520)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "<p>The cellular localisation of this product has been verified in ICC/IF.</p>" }, "Mouse": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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제품 세부 정보

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
Avoid freeze / thaw cycle|Store in the dark
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

CTP synthase also known as CTPS or the CTP protein is an enzyme involved in pyrimidine metabolism. It catalyzes the conversion of UTP to CTP an essential step in nucleotide biosynthesis. The enzyme exists in two isoforms CTPS1 and CTPS2 with both found in various tissues but CTPS2 has a higher expression in the brain. CTP synthase has a molecular mass of roughly 70 kDa. CTPS can assemble into filamentous structures under certain conditions influencing its enzymatic activity. These filaments sometimes referred to as cytoophidia are observed in many types of cells.
Biological function summary

CTP synthase plays a significant role in the regulation of nucleotide pools. The enzyme ensures the balance of CTP levels necessary for DNA RNA and phospholipid synthesis acting as a rate-limiting step in these processes. CTPS is part of larger enzymatic complexes that aid in cellular response to nutrient availability and DNA damage. Allosteric regulation and product feedback inhibition modulate CTP synthase activity maintaining cellular homeostasis and a proper supply of CTP for various biosynthetic pathways.

Pathways

CTP synthase is an important component in the pyrimidine biosynthesis pathway essential for nucleic acid and lipid metabolism. The enzyme's activity is tightly linked to the salvage pathways collaborating with enzymes like ribonucleotide reductase to regulate deoxyribonucleotide levels for DNA replication and repair. These interactions highlight the enzyme's involvement in cellular proliferation and response to metabolic stresses underpinning its regulatory function in metabolic pathways.

CTP synthase activity disorders relate to cancer and neurodegeneration. Dysregulation of CTPS is associated with increased proliferation seen in certain cancers given its role in nucleotide biosynthesis. Research also indicates a link between CTPS activity and neurodegenerative diseases as nucleotide imbalance affects neural function. Additionally interactions with proteins involved in cell cycle control and apoptosis such as p53 and Rb further highlight CTPS's role in disease etiology influencing therapeutic strategies targeting these diverse conditions.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

CTP synthase involved in the de novo synthesis of CTP, a precursor of DNA, RNA and phospholipids (PubMed : 16179339, PubMed : 17189248, PubMed : 17463002, PubMed : 24870241, PubMed : 28459447, PubMed : 34583994). Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as a source of nitrogen (PubMed : 16179339, PubMed : 24870241, PubMed : 28459447, PubMed : 34583994). CTPS1 CTP synthase activity plays a crucial role in the proliferation of activated lymphocytes and immunity; additional CTP being required to meet increased demand for DNA, RNA and lipid membrane biosynthesis in proliferating lymphocytes (PubMed : 24870241, PubMed : 8530356). In addition to CTP synthase activity, also acts as a protein deamidase that catalyzes the side chain deamidation of specific asparagine residues of proteins to aspartate (PubMed : 40240600). Acts as a negative regulator of innate immunity by mediating deamidation of 'Asn-85' of IRF3, preventing IRF3 from binding DNA (By similarity). Facilitates chromatin relaxation in response to DNA damage by mediating deamidation of 'Asn-76' and 'Asn-77' of histone H1, thereby promoting subsequent acetylation of histone H1 at 'Lys-75' (H1K75ac), increasing chromatin accessibility to facilitate the recruitment of DNA repair proteins (PubMed : 40240600).
See full target information CTPS1
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of clinical and translational hepatology 13:555-567 PubMed40937080

2025

CCNE1 Promotes the Progression of Hepatic Precancerous Lesion and the Malignant Phenotype of Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Zhang,Xue Hu,Lichao Yao,Wenzhi Guo
제품이 사용된 논문 모두 보기
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Product promise

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