PE/Cy5® Anti-CD11b antibody [M1/70]

• Flow Cyt

AbReview19983****

Flow Cytometry - PE/Cy5® Anti-CD11b antibody [M1/70] (AB25533)

ab25533 staining CD11b in Human leukocytes by Flow Cytometry. Cells were prepared for staining after red blood cell lysis and were fixed in paraformaldehyde. The sample was incubated with the primary antibody (1/50 in PBS/BSA 1%) for 30 minutes at 4°C. Gating strategy : Neutrophils.

This image is courtesy of an anonymous Abreview

• Flow Cyt

Supplier Data

Flow Cytometry - PE/Cy5® Anti-CD11b antibody [M1/70] (AB25533)

Flow Cytometry analysis of C57Bl/6 bone marrow cells labeling CD11b with ab25533 at 0.125 μg (solid line) or Rat IgG2b (PE/Cy5^®) isotype control at 0.125 μg (dashed line).

• Flow Cyt

Unknown

Flow Cytometry - PE/Cy5® Anti-CD11b antibody [M1/70] (AB25533)

Scatter plot showing mouse blood leukocyte cells stained with ab25533 (green). The cells were harvested from blood drawn from mice and prepared in PBS/BSA 1%. Cells were fixed in formaldehyde. The cells were then incubated in 1x PBS / 1% BSA to block non-specific protein-protein interactions followed by the antibody (1/100) for 30 min at 4ºC. Isotype control antibody (red) was used under the same conditions.

• Flow Cyt

AbReview28847****

Flow Cytometry - PE/Cy5® Anti-CD11b antibody [M1/70] (AB25533)

ab25533 staining CD11b in Rat splenocytes by Flow Cytometry. Rat spleen was homogenized and red blood cells were lysed in PBS + 1% BSA and 0.01% sodium azide. The sample was incubated with the primary antibody (1/100 in PBS + 1% BSA and 0.01% sodium azide) for 1 hour at 4°C.
Gating Strategy : Live Macrophages.

This image is courtesy of an anonymous Abreview