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AB233572

PE/Cy5® Anti-CD80 antibody [MEM-233]

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Anti-CD80 antibody [MEM-233] conjugated to PE/Cy5® - suitable for flow cytometry, and reacts with human samples. Clone [MEM-233] is a mouse monoclonal antibody cited in 51 publications.

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Flow Cytometry - PE/Cy5® Anti-CD80 antibody [MEM-233] (AB233572)
  • Flow Cyt

Supplier Data

Flow Cytometry - PE/Cy5® Anti-CD80 antibody [MEM-233] (AB233572)

Anti-Hu CD80 PE-Cy™5 antibody (concentration in sample 3 µg/ml, red-filled histogram) binds specifically to CD80 on surface of CD80 transfected P815 cells (upper panel), but not to surface of non-transefected P815 cells (lower panel). Level of non-specific binding was assessed using Mouse IgG1 isotype control PE-Cy™5 antibody (MOPC-21) under same conditions (concentration in sample 3 µg/ml, black-dashed histogram).

관련 conjugated 항체와 다양한 조성의 항체 (6)

  • 660 APC

    APC Anti-CD80 antibody [MEM-233]

  • 723 Alexa Fluor® 700

    Alexa Fluor® 700 Anti-CD80 antibody [MEM-233]

  • 519 FITC

    FITC Anti-CD80 antibody [MEM-233]

  • 578 PE

    PE Anti-CD80 antibody [MEM-233]

  • 778 PE/Cy7®

    PE/Cy7® Anti-CD80 antibody [MEM-233]

  • 675 PerCP

    PerCP Anti-CD80 antibody [MEM-233]

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

MEM-233

Isotype

IgG1

Conjugation

PE/Cy5®

Excitation/Emission

Ex: 496nm, Em: 667nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Recombinant Fragment Protein within Human CD80. The exact immunogen used to generate this antibody is proprietary information.

P33681

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대체품

Primary Antibodies

AB134120

Anti-CD80 antibody [EPR1157(2)]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD80 antibody [EPR1157(2)] (AB134120)

  • 4
  • 2
Primary Antibodies

AB222702

Anti-CD80 antibody [EPR22127-251]

20ul selling size|RabMAb|Recombinant|Trial Size|Trial Size|Trial Size|Trial Size

Flow Cytometry - Anti-CD80 antibody [EPR22127-251] (AB222702)

  • 0
  • 0
Primary Antibodies

AB224797

Anti-CD80 antibody [EPR1157(2)] - Low endotoxin, Azide free

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD80 antibody [EPR1157(2)] - Low endotoxin, Azide free (AB224797)

  • 0
  • 1
Primary Antibodies

AB225674

Anti-CD80 antibody [EPR22183]

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-CD80 antibody [EPR22183] (AB225674)

  • 5
  • 1
Primary Antibodies

AB238481

Anti-CD80 antibody [EPR22127-317]

20ul selling size|RabMAb|Recombinant

Western blot - Anti-CD80 antibody [EPR22127-317] (AB238481)

  • 0
  • 0
Primary Antibodies

AB238648

Anti-CD80 antibody [EPR22183] - BSA and Azide free

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-CD80 antibody [EPR22183] - BSA and Azide free (AB238648)

  • 0
  • 0
Primary Antibodies

AB238649

Anti-CD80 antibody [EPR22127-251] - BSA and Azide free

RabMAb|Recombinant

Flow Cytometry - Anti-CD80 antibody [EPR22127-251] - BSA and Azide free (AB238649)

  • 0
  • 0

보완 제품

Assay Kits

AB256392

Human CD80 ELISA Kit (B7-1)

Recombinant|SimpleStep

Sandwich ELISA - Human CD80 ELISA Kit (B7-1) (AB256392)

  • 0
  • 0
Primary Antibodies

AB288358

Alexa Fluor® 647 Anti-CD86 antibody [EPR21962]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-CD86 antibody [EPR21962] (AB288358)

  • 0
  • 0
Primary Antibodies

AB302698

Alexa Fluor® 647 Anti-CD14 antibody [SP192]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD14 antibody [SP192] (AB302698)

  • 0
  • 0
Primary Antibodies

AB270647

FITC Anti-CD206 antibody [15-2]

Flow Cytometry - FITC Anti-CD206 antibody [15-2] (AB270647)

  • 0
  • 0
Primary Antibodies

AB305048

Alexa Fluor® 647 Anti-CD8 alpha antibody [CAL66]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD8 alpha antibody [CAL66] (AB305048)

  • 5
  • 1
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "4 µL for 10^6 Cells", "FlowCyt-species-notes": "<p></p>" }, "Transfected cell line": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "4 µL for 10^6 Cells", "FlowCyt-species-notes": "<p></p>" } } }
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제품 세부 정보

This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy® and CyDye® are trademarks of Cytiva.
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Size-exclusion chromatography
보관 버퍼
pH: 7.4 Preservative: 0.0975% Sodium azide Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
Store in the dark
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

CD80 also known as B7-1 is a cell surface protein with a molecular weight of approximately 60 kDa. It is expressed on antigen-presenting cells such as dendritic cells macrophages and B cells. The CD80 protein plays a mechanical role in immune response modulation by providing necessary signals for T cell activation and survival. CD80 interacts with receptors CD28 and CTLA-4 on T cells serving as a costimulatory molecule important for productive immune responses.
Biological function summary

CD80 plays a significant role in the immune system's ability to distinguish between self and non-self. It belongs to the B7 family and participates in the formation of immunological synapses between T cells and antigen-presenting cells. This interaction is essential for triggering T cell proliferation and cytokine production forming a part of the adaptive immune response. CD80's expression increases in response to pathogen recognition enhancing the immune system's efficiency.

Pathways

CD80 is integral to the regulation of T cell-mediated immune responses. It is involved in the CD28 co-stimulatory pathway which is vital for T cell activation and function. In this pathway CD80 interacts with proteins like CD28 and CTLA-4 to modulate immune activation and regulate immune tolerance. The proper function of CD80 within this pathway ensures a balanced immune reaction preventing autoimmunity while allowing effective defense against pathogens.

CD80 plays a role in autoimmune conditions and transplantation rejection. Its overexpression or dysregulation can contribute to diseases such as rheumatoid arthritis where it modifies T cell response and inflammation. Additionally CD80 is implicated in organ transplantation influencing graft rejection outcomes due to its role in modulating immune activation. Understanding CD80 interactions with CTLA-4 and CD28 offers insight into these conditions providing potential targets for therapeutic intervention.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Costimulatory molecule that belongs to the immunoglobulin superfamily that plays an important role in T-lymphocyte activation (PubMed : 38467718). Acts as the primary auxiliary signal augmenting the MHC/TCR signal in naive T-cells by acting as a ligand for the CD28 receptor which is constitutively expressed on the cell surface of T-cells (PubMed : 12196291). In turn, activates different signaling pathways such as NF-kappa-B or MAPK leading to the production of different cytokines (PubMed : 10438913). Also acts as an inhibitor of T-cell activation by acting as a ligand for CTLA4, a decoy receptor, thereby blocking CD28-mediated T-cell priming (PubMed : 10583602, PubMed : 11279502). In addition, CD28/CD80 costimulatory signal stimulates glucose metabolism and ATP synthesis of T-cells by activating the PI3K/Akt signaling pathway (PubMed : 12121659). Also acts as a regulator of PDL1/PDCD1 interactions to limit excess engagement of PDL1 and its inhibitory role in immune responses (PubMed : 36727298). Expressed on B-cells, plays a critical role in regulating interactions between B-cells and T-cells in both early and late germinal center responses, which are crucial for the generation of effective humoral immune responses (By similarity).. (Microbial infection) Acts as a receptor for adenovirus subgroup B.
See full target information CD80

대체 명칭 보기

CD80, CD28LG, CD28LG1, LAB7, T-lymphocyte activation antigen CD80, Activation B7-1 antigen, BB1, CTLA-4 counter-receptor B7.1, B7
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of inflammation (London, England) 22:41 PubMed41057834

2025

Baicalein inhibits mycobacterium tuberculosis-induced macrophage M1 polarization depending on the regulation of YY1/RAB10/TLR4 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Zhou,Shuanghua Chen,Linfei Shu,Qian Wang,Ting Yuan,Yangjing Ou,Ling Qing,Xiaojin He
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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