AB239317

PE/Cy7® Anti-CD45 antibody [MEM-28]

Be the first to review this product! 리뷰 제출

(3 제품이 사용된 논문 )

Anti-CD45 antibody [MEM-28] conjugated to PE/Cy7® - suitable for flow cytometry, and reacts with human samples. Clone [MEM-28] is a mouse monoclonal antibody cited in 33 publications.

Design your panel with our interactive flow cytometry panel builder. Click here.

2 이미지

Flow Cytometry - PE/Cy7® Anti-CD45 antibody [MEM-28] (AB239317)

Flow Cyt

Supplier Data

Flow Cytometry - PE/Cy7® Anti-CD45 antibody [MEM-28] (AB239317)

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD45 (MEM-28) PE-Cy™7 antibody (4 µl reagent / 100 µl of peripheral whole blood).

Flow Cyt

Supplier Data

Flow Cytometry - PE/Cy7® Anti-CD45 antibody [MEM-28] (AB239317)

Separation of human CD45 positive lymphocytes (red-filled) from CD45 negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD45 (MEM-28) PE-Cy™7 antibody (4 µl reagent / 100 µl of peripheral whole blood).

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

MEM-28

Isotype

IgG1

Conjugation

PE/Cy7®

Excitation/Emission

Ex: 565nm, Em: 778nm

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing PTPRC protein. The exact immunogen used to generate this antibody is proprietary information.

P08575

Epitope

Extracellular region.

Specificity

<div class="product-row"> <div class="product-row-text">The antibody MEM-28 reacts with an extracellular epitope on all alternative forms of human CD45 antigen (Leukocyte Common Antigen), a 180-220 kDa single chain type I transmembrane protein expressed at high level on all cells of hematopoietic origin, except erythrocytes and platelets.</div> </div> <div class="product-row"> </div>

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "4 µL for 10^6 Cells", "FlowCyt-species-notes": "<p><div class=\"product-row\"><div class=\"product-row-text\">The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 10<sup>6</sup> cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.</div></div><div class=\"product-row\"> </div>" }, "Horse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "4 µL for 10^6 Cells", "FlowCyt-species-notes": "<p><div class=\"product-row\"><div class=\"product-row-text\">The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 10<sup>6</sup> cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.</div></div><div class=\"product-row\"> </div>" } } }

style

left-column

제품 세부 정보

This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy® and CyDye® are trademarks of Cytiva.

style

left-column

특성 및 보관 정보

제형

Liquid

Purification 테크닉

Size-exclusion chromatography

Purification 관련 사항

Purified antibody is conjugated with tandem dye PE/Cy7 ® under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

보관 버퍼

pH: 7.4 Preservative: 0.0975% Sodium azide Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

Store in the dark

style

left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

CD45 also known as Protein tyrosine phosphatase receptor type C (PTPRC) is a transmembrane glycoprotein with a molecular mass ranging between 180-240 kDa depending on its isoform. It is expressed on the surface of almost all hematopoietic cells except for mature erythrocytes and platelets. CD45 has a critical role in regulating antigen receptor signaling by modifying kinases involved in signal transduction and it is essential in lymphocyte development and activation. Because of its broad expression on immune cells CD45 is a valuable marker for differentiating various immune cell types in assays like flow cytometry and immunohistochemistry (IHC) often referred to as CD45 stain.

Biological function summary

CD45 acts by regulating tyrosine phosphorylation in the immune cell signaling context participating directly in signal transduction. It functions by dephosphorylating specific phosphotyrosine residues on various proteins modulating the signaling threshold required for lymphocyte activation. Although not known to be part of a protein complex CD45 itself shows isoform variation that associates with specific immune cell types impacting their function. Importantly CD45 interacts with multiple signaling molecules to affect cell growth and differentiation.

Pathways

Scientists associate CD45 with signaling pathways such as the T-cell receptor (TCR) and B-cell receptor (BCR) signaling. CD45 modulates the activity of Src family kinases important elements in these pathways making it essential for effective immune response and tolerance. Furthermore interactions between CD45 and proteins like Lck in T-cells or Lyn in B-cells highlight its pivotal role in executing its signaling functions.

Scientists often link CD45 to immune-related conditions including autoimmunity and leukemia. In autoimmune diseases altered CD45 expression or activity can disrupt normal immune function contributing to pathogenesis. In leukemia CD45 expression levels can assist in disease classification and prognosis as it interacts with proteins involved in cell cycle regulation. The anti-CD45 antibodies can provide diagnostic and therapeutic avenues highlighting their utility in disease management.

style

left-column

style

left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

타겟 정보

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor (PubMed : 35767951). Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity). Interacts with CLEC10A at antigen presenting cell-T cell contact; CLEC10A on immature dendritic cells recognizes Tn antigen-carrying PTPRC/CD45 receptor on effector T cells and modulates T cell activation threshold to limit autoreactivity.. (Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation.

See full target information PTPRC

대체 명칭 보기

CD45, PTPRC, Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, T200, L-CA

style

left-column

제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cells 14: PubMed40643462

2025

Insulin Modulates NK Cell Activity in Liver Fibrosis MASH Patients via the STING Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Johnny Amer,Ahmad Salhab,Amiram Ariel,Rifaat Safadi

Molecular medicine (Cambridge, Mass.) 31:244 PubMed40542350

2025

Effect of anti-CD4 mAb induced by inhibiting B cell disorder on immune reconstruction of HIV-infected immunological non-responders.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Ouyang,Kang Wu,Lei Fu,Panpan Yi,Da Cheng,Xiaoyu Fu

Journal of biochemical and molecular toxicology 34:e22436 PubMed31953908

2020

SENP1/HIF-1α axis works in angiogenesis of human dental pulp stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Zhou,Cong Sun

제품이 사용된 논문 모두 보기

style

left-column

style

left-column

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PE/Cy7® Anti-CD45 antibody [MEM-28]

Flow Cytometry - PE/Cy7® Anti-CD45 antibody [MEM-28] (AB239317)

Flow Cytometry - PE/Cy7® Anti-CD45 antibody [MEM-28] (AB239317)

관련 conjugated 항체와 다양한 조성의 항체 (9)

주요 정보

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Epitope

Specificity

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (3)

Product promise