AB90751

PE Anti-ENPP3/B10 antibody [NP4D6]

Name: PE Anti-ENPP3/B10 antibody [NP4D6]
Brand: Abcam Limited
SKU: AB90751
Rating: 4 (3 reviews)

(3 리뷰들)

(1 출판물)

Anti-ENPP3/B10 antibody [NP4D6] conjugated to PE - suitable for flow cytometry, intracellular flow cytometry, ICC/IF, and reacts with human samples. Clone [NP4D6] is a mouse monoclonal antibody cited in 18 publications.

6 이미지

Flow Cytometry - PE Anti-ENPP3/B10 antibody [NP4D6] (AB90751)

Flow Cyt

Unknown

Flow Cytometry - PE Anti-ENPP3/B10 antibody [NP4D6] (AB90751)

ab90751 staining ENPP3/B10 on the surface of human basophils isolated from allergen-stimulated whole blood by flow cytometery.

Immunocytochemistry/ Immunofluorescence - PE Anti-ENPP3/B10 antibody [NP4D6] (AB90751)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - PE Anti-ENPP3/B10 antibody [NP4D6] (AB90751)

Surface staining of activated human basophil by FITC-conjugated anti-CD63 (clone MEM-259, ab18235, green) and PE-conjugated anti-ENPP3/B10 (clone NP4D6, ab90751, red); merged signal yellow. DNA visualized by DAPI (blue).

Supplier Data

Western blot - PE Anti-ENPP3/B10 antibody [NP4D6] (AB90751)

Blocking and diluting buffer : 5% NFDM/TBST We are not sure how to define the extra band below band of interest.

All lanes:

Western blot - Anti-SENP2 antibody [EPR4356(2)] (<a href='/ko/products/primary-antibodies/senp2-antibody-epr43562-ab124724'>ab124724</a>) at 1/10000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg

Lane 2:

MDA-MB-435S (Human breast adenocarcinoma epithelial cell) whole cell lysate at 15 µg

Lane 3:

293T (Human embryonic kidney epithelial cell) whole cell lysate at 15 µg

Lane 4:

NIH: OVCAR-3 (Human ovary adenocarcinoma epithelial cell) whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - PE Anti-ENPP3/B10 antibody [NP4D6] (ab90751) at 1/20000 dilution

Predicted band size: 68 kDa

Observed band size: 68 kDa

false

Exposure time: 40s

Supplier Data

Western blot - PE Anti-ENPP3/B10 antibody [NP4D6] (AB90751)

Blocking and Diluting Buffer : 5% NFDM /TBST 87 kDa ProPC1/3; 66 kDa mature form (PMID : 26778167) Lane1 Expsoure Time : 20 seconds Lane2 and Lane3 were developed using a high sensitivity ECL substrate.

All lanes:

Western blot - Anti-PC1/3 antibody [EPR21908] (<a href='/ko/products/primary-antibodies/pc1-3-antibody-epr21908-ab220363'>ab220363</a>) at 1/1000 dilution

Lane 1:

Beta-TC-6 (mouse pancreas insulinoma beta cell) whole cell lysate at 20 µg

Lane 2:

Mouse pancreas lysate at 20 µg

Lane 3:

Mouse brain lysate at 20 µg

Secondary

All lanes:

Western blot - PE Anti-ENPP3/B10 antibody [NP4D6] (ab90751) at 1/20000 dilution

Predicted band size: 87 kDa

Observed band size: 66 kDa

false

Exposure time: 80s

Supplier Data

Western blot - PE Anti-ENPP3/B10 antibody [NP4D6] (AB90751)

IBA1 is a relatively minor protein of brain and is much more abundant in spleen (PMID : 8912632, PMID : 29232670). We suggest loading higher amount of brain lysate or using lower dilution of antibody for detecting signal in brain related lysates.

All lanes:

Western blot - Anti-Iba1 antibody [EPR16589] - Microglia marker (<a href='/ko/products/primary-antibodies/iba1-antibody-epr16589-microglia-marker-ab178847'>ab178847</a>) at 1/1000 dilution

Lane 1:

Mouse spleen tissue lysate at 20 µg

Lane 2:

Mouse brain tissue lysate at 20 µg

Lane 3:

Mouse hippocampus tissue at 20 µg

Lane 4:

Rat spleen tissue lysate at 20 µg

Lane 5:

Rat brain tissue lysate at 20 µg

Lane 6:

Rat hippocampus tissue lysate at 20 µg

Secondary

All lanes:

Western blot - PE Anti-ENPP3/B10 antibody [NP4D6] (ab90751) at 1/20000 dilution

Predicted band size: 16 kDa

Observed band size: 17 kDa

false

Exposure time: 40s

Supplier Data

Western blot - PE Anti-ENPP3/B10 antibody [NP4D6] (AB90751)

Blocking and diluting buffer : 5% NFDM/TBST Lysate/protein at 0.01 µg per lane.

All lanes:

Western blot - Anti-SENP2 antibody [EPR4356(2)] (<a href='/ko/products/primary-antibodies/senp2-antibody-epr43562-ab124724'>ab124724</a>) at 1/10000 dilution

Secondary

All lanes:

Western blot - PE Anti-ENPP3/B10 antibody [NP4D6] (ab90751) at 1/20000 dilution

Predicted band size: 68 kDa

Observed band size: 46 kDa

false

Exposure time: 20s

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

NP4D6

Isotype

IgG1

Conjugation

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

Reacts with

Human

Applications

Flow Cyt, ICC/IF

applications

Immunogen

Cell preparation containing ENPP3 protein. The exact immunogen used to generate this antibody is proprietary information.

O14638

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "20 µL for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/ko/products/primary-antibodies/pe-mouse-igg1-b11-6-isotype-control-ab91357'>ab91357</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }

style

left-column

제품 세부 정보

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions.

style

left-column

특성 및 보관 정보

제형

Liquid

Purification 테크닉

Size-exclusion chromatography

Purification 관련 사항

The unconjugated antibody was purified by protein A (G) affinity chromatography (>95% by SDS-PAGE). After conjugation, the antibody was purified by size-exclusion chromatography and adjusted for direct use.

보관 버퍼

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS, 0.2% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

style

left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The ENPP3/B10 protein also known as Ectonucleotide Pyrophosphatase/Phosphodiesterase 3 is an enzyme that plays a role in nucleotide metabolism by hydrolyzing phosphodiester and phosphosulfate bonds. This protein has a molecular mass of around 100 kDa. ENPP3 is mainly expressed on the surface of epithelial cells and you find it in tissues like the kidney liver and lung. In some contexts immune cells such as mast cells also show its expression.

Biological function summary

ENPP3 regulates the availability of extracellular nucleotides and phosphate metabolites. It is not typically considered part of a larger complex and rather contributes to various processes independently. By modulating concentrations of these extracellular molecules ENPP3 impacts cell signaling and nucleotide homeostasis which can affect cell proliferation and differentiation.

Pathways

This enzyme engages in the purinergic signaling pathway and can influence the MAPK/ERK pathway. Through its enzymatic action on nucleotides ENPP3 helps regulate signaling molecules which participate in processes such as cell growth and immune responses. Proteins related to these pathways include ATP-binding proteins like P2Y receptors and kinases involved in MAPK/ERK signaling.

ENPP3's function links it to conditions such as allergy and asthma as its expression on mast cells affects histamine release. Changes in its activity are also noted in certain cancers where its role in signaling pathways can contribute to uncontrolled cell proliferation. In these disease contexts the protein interacts with other signaling molecules like cytokine receptors and oncogenic proteins which can modulate or exacerbate disease progression.

style

left-column

style

left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

타겟 정보

Hydrolase that metabolizes extracellular nucleotides, including ATP, GTP, UTP and CTP (PubMed : 29717535, PubMed : 9344668). Also hydrolyzes extracellular 2',3'-cGAMP (cyclic GMP-AMP), a second messenger that activates TMEM173/STING and triggers type-I interferon production, and is therefore involved in the regulation of innate immune response (PubMed : 38749434). 2',3'-cGAMP appears to be further processed to GMP, AMP and inorganic phosphate (PubMed : 38749434). Limits mast cells and basophils response during inflammation and during the chronic phases of allergic responses by eliminating extracellular ATP, a signaling molecule activating these cells in an autocrine manner. Metabolizes extracellular ATP in the lumen of the small intestine, and thereby prevents ATP-induced apoptosis of intestinal plasmacytoid dendritic cells (By similarity). Has a broad specificity and can also hydrolyze UDP-GlcNAc into UMP and GlcNAc-1-phosphate and potentially several other intracellular nucleotide sugars, including UDP-GalNAc, CMP-NeuAc, GDP-Fuc, and UDP-GlcA. Thereby, could modulate glycan biosynthesis and protein glycosylation (By similarity). Can hydrolyze extracellular dinucleoside polyphosphates, including the vasoactive adenosine polyphosphates as well (PubMed : 12846830). In addition, displays an alkaline phosphodiesterase activity in vitro (PubMed : 11342463).

See full target information ENPP3

대체 명칭 보기

CD203c, PDNP3, ENPP3, Ectonucleotide pyrophosphatase/phosphodiesterase family member 3, E-NPP 3, NPP3, Alkaline phosphodiesterase I, Dinucleoside polyphosphatase, Nucleotide diphosphatase, Nucleotide pyrophosphatase, Phosphodiesterase I beta, Phosphodiesterase I/nucleotide pyrophosphatase 3, NPPase, PD-Ibeta

style

left-column

제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cancer genomics & proteomics 17:141-149 PubMed32108036

2020

KIF15 Expression in Tumor-associated Monocytes Is a Prognostic Biomarker in Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Akihiro Kitagawa,Takaaki Masuda,Junichi Takahashi,Taro Tobo,Miwa Noda,Yosuke Kuroda,Qingjiang Hu,Yuta Kouyama,Yuta Kobayashi,Shotaro Kuramitsu,Kuniaki Sato,Atsushi Fujii,Yukihiro Yoshikawa,Hiroaki Wakiyama,Dai Shimizu,Yusuke Tsuruda,Hidetoshi Eguchi,Yuichiro Doki,Masaki Mori,Koshi Mimori

제품이 사용된 논문 모두 보기

style

left-column

style

left-column

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PE Anti-ENPP3/B10 antibody [NP4D6]

Flow Cytometry - PE Anti-ENPP3/B10 antibody [NP4D6] (AB90751)

Immunocytochemistry/ Immunofluorescence - PE Anti-ENPP3/B10 antibody [NP4D6] (AB90751)

Western blot - PE Anti-ENPP3/B10 antibody [NP4D6] (AB90751)

Western blot - PE Anti-ENPP3/B10 antibody [NP4D6] (AB90751)

Western blot - PE Anti-ENPP3/B10 antibody [NP4D6] (AB90751)

Western blot - PE Anti-ENPP3/B10 antibody [NP4D6] (AB90751)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (1)

Product promise