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AB326525

PE Anti-Fas Ligand antibody [NOK-1]

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Mouse Monoclonal Fas Ligand antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Transfected cell line - Human samples.
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Flow Cytometry - PE Anti-Fas Ligand antibody [NOK-1] (AB326525)
  • Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-Fas Ligand antibody [NOK-1] (AB326525)

ab326525 antibody (concentration in sample 1.67 μg/ml, red-filled histogram) binds specifically to CD178 expressed on surface of Fas-L transfected L5178Y cells (upper panel), but not to surface of non-transfected L5178Y cells (lower panel). Level of non-specific binding was assessed using Mouse IgG1 isotype control PE antibody (MOPC-21) under same conditions (concentration in sample 1.67 μg/ml, black-dashed histogram).

관련 conjugated 항체와 다양한 조성의 항체 (4)

  • Carrier free

    Anti-Fas Ligand antibody [NOK-1] - Low endotoxin, Azide free

  • 660 APC

    APC Anti-Fas Ligand antibody [NOK-1]

  • 387 mFluor™ UV375

    mFluor™ UV375 Anti-CD178 antibody [NOK-1]

  • 454 mFluor™ Violet 450

    mFluor™ Violet 450 Anti-CD178 antibody [NOK-1]

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

NOK-1

Isotype

IgG1

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Specificity

The mouse monoclonal antibody NOK-1 recognizes an extracellular epitope of CD178 / Fas-L, an approximately 40 kDa transmembrane glycoprotein expressed on neutrophils, monocytes, and activated T and NK cells.

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보완 제품

Primary Antibodies

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Alexa Fluor® 488 Anti-CD45 antibody [EP322Y]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-CD45 antibody [EP322Y] (AB200315)

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Primary Antibodies

AB310299

Alexa Fluor® 647 Anti-CD16 antibody [SP175]

RabMAb|Recombinant

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Primary Antibodies

AB314291

APC Anti-NKG2D antibody [EPR22935-131]

RabMAb|Recombinant

Flow Cytometry - APC Anti-NKG2D antibody [EPR22935-131] (AB314291)

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Primary Antibodies

AB281758

Alexa Fluor® 488 Anti-LAMP1 antibody [EPR24395-31] - Lysosome Marker

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-LAMP1 antibody [EPR24395-31] - Lysosome Marker (AB281758)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Transfected cell line - Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.</p>" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Size-exclusion chromatography
Purification 관련 사항
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
보관 버퍼
pH: 7.4 Preservative: 0.098% Sodium azide Constituents: PBS, 0.2% BSA
배송 시 보관 조건
Ambient - Can Ship with Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
Do Not Freeze|Store in the dark
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells (PubMed : 26334989, PubMed : 9228058). Involved in cytotoxic T-cell-mediated apoptosis, natural killer cell-mediated apoptosis and in T-cell development (PubMed : 7528780, PubMed : 9228058, PubMed : 9427603). Initiates fratricidal/suicidal activation-induced cell death (AICD) in antigen-activated T-cells contributing to the termination of immune responses (By similarity). TNFRSF6/FAS-mediated apoptosis also has a role in the induction of peripheral tolerance (By similarity). Binds to TNFRSF6B/DcR3, a decoy receptor that blocks apoptosis (PubMed : 27806260).. Tumor necrosis factor ligand superfamily member 6, soluble form. Induces FAS-mediated activation of NF-kappa-B, initiating non-apoptotic signaling pathways (By similarity). Can induce apoptosis but does not appear to be essential for this process (PubMed : 27806260).. FasL intracellular domain. Cytoplasmic form induces gene transcription inhibition.
See full target information FASLG

대체 명칭 보기

CD178, APT1LG1, CD95L, FASL, TNFSF6, FASLG, Tumor necrosis factor ligand superfamily member 6, Apoptosis antigen ligand, CD95 ligand, Fas antigen ligand, APTL, CD95-L, Fas ligand, FasL
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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