PE Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668]

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - PE Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] (AB326146)

Flow cytometry scatter plots showing untreated human peripheral mononuclear cells (PBMCs) (top) and PBMCs activated with 5 µg/ml PHA for 18h (bottom), either stained with ab326146 (right) or isotype control (left). The cells were fixed and permeabilised using BD CytoFix/CytoPerm™ (20 min). The cells were incubated in 1x PBS containing 10 µg/ml human IgG, 10% normal goat serum and BD Pharmingen™ MonoBlock™ Leukocyte Staining Buffer (1/10) to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab326146) (1x 106 in 100µl at 0.2 µg/ml (1/2500)) for 30min on ice. Isotype control antibody was Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) (left). The cells were simultaneously stained with CD3. Acquisition of >30000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.This data was acquired using a Beckman Coulter CytoFlexLX flow cytometer.