JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB275699

PE Anti-Hsp27 antibody [155]

Be the first to review this product! 리뷰 제출

|

(1 출판물)

Rabbit Recombinant Monoclonal Hsp27 antibody - conjugated to PE. Suitable for Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human HSPB1 aa 1 to C-terminus.

대체 명칭 보기

HSP27, HSP28, HSPB1, Heat shock protein beta-1, HspB1, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, Heat shock protein family B member 1, Stress-responsive protein 27, HSP 27, SRP27

1 이미지
Flow Cytometry (Intracellular) - PE Anti-Hsp27 antibody [155] (AB275699)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - PE Anti-Hsp27 antibody [155] (AB275699)

Intracellular flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling HSP27 with ab275699 (Black) compared with isotype control (Grey). The Fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

155

Isotype

IgG

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra)

applications

Immunogen

Recombinant Fragment Protein within Human HSPB1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P04792

style
left-column

보완 제품

Assay Kits

AB108862

Human Heat Shock Protein 27 ELISA Kit (HSP27)

Sandwich ELISA - Human Heat Shock Protein 27 ELISA Kit (HSP27) (AB108862)

  • 0
  • 0
Proteins & Peptides

AB48740

Recombinant Human Hsp27 protein (Tag Free)

Western blot - Recombinant Human Hsp27 protein (Tag Free) (AB48740)

  • 0
  • 0
Multiplex Kits

AB193661

Human HSP Antibody Array - Membrane (9 Targets)

Multiplex Protein Detection - Human HSP Antibody Array - Membrane (9 Targets) (AB193661)

  • 0
  • 0
Primary Antibodies

AB40742

HRP Anti-alpha Tubulin antibody [DM1A] - Loading Control

BOND RX™ Validated

Western blot - HRP Anti-alpha Tubulin antibody [DM1A] - Loading Control (AB40742)

  • 5
  • 5
Primary Antibodies

AB2728

Anti-Cytohesin 2 antibody [10A12]

Immunocytochemistry/ Immunofluorescence - Anti-Cytohesin 2 antibody [10A12] (AB2728)

  • 0
  • 0
style
left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
style
left-column
style
left-column

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
Preservative: 0.09% Sodium azide Constituents: 0.5% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
Do Not Freeze|Store in the dark
style
left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Hsp27 also known as HSPB1 is a small heat shock protein with a molecular weight of approximately 27 kilodaltons. This protein is expressed in various tissues including muscle heart and brain. It functions as a molecular chaperone that stabilizes unfolded proteins preventing their aggregation. Hsp27 undergoes phosphorylation at specific residues which modulates its chaperone activity and interaction with other proteins.
Biological function summary

Hsp27 plays a critical role in cellular stress response by regulating actin cytoskeleton dynamics and inhibiting apoptosis. It forms part of a complex that includes other proteins such as alphaB-crystallin. This complex facilitates the reorganization of proteins under stress conditions enhancing cell survival during oxidative stress or thermal shock. Hsp27 also modulates inflammatory responses and has been shown to affect cell migration.

Pathways

Hsp27 integrates into the apoptosis and inflammation pathways. It interacts with apoptotic machinery such as caspase proteins to protect cells by hindering apoptosome formation. Additionally Hsp27 can engage with pathways involving the nuclear factor-kappa B (NF-kB) impacting inflammatory signaling. CPTC (carboxyl-pyrene-trioctylamine) can modulate these pathways by altering Hsp27 function and interactions.

Hsp27 has connections to neurodegenerative diseases and cancer. In neurodegenerative conditions such as Alzheimer's disease its chaperone activity is thought to protect neurons from misfolded protein aggregates. In cancer Hsp27 supports tumor cell survival and resistance to chemotherapy by interacting with proteins like Akt and p53. These interactions highlight the complex role of Hsp27 in modulating cellular responses in various pathological states.
style
left-column
style
left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

타겟 정보

Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state (PubMed : 10383393, PubMed : 20178975). Plays a role in stress resistance and actin organization (PubMed : 19166925). Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins (PubMed : 23728742).
See full target information HSPB1
style
left-column

제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nature biotechnology 43:329-344 PubMed38744947

2024

Generation of allogeneic CAR-NKT cells from hematopoietic stem and progenitor cells using a clinically guided culture method.

Applications

Unspecified application

Species

Unspecified reactive species

Yan-Ruide Li,Yang Zhou,Jiaji Yu,Yu Jeong Kim,Miao Li,Derek Lee,Kuangyi Zhou,Yuning Chen,Yichen Zhu,Yu-Chen Wang,Zhe Li,Yanqi Yu,Zachary Spencer Dunn,Wenbin Guo,Xinjian Cen,Tiffany Husman,Aarushi Bajpai,Adam Kramer,Matthew Wilson,Ying Fang,Jie Huang,Shuo Li,Yonggang Zhou,Yuchong Zhang,Zoe Hahn,Enbo Zhu,Feiyang Ma,Calvin Pan,Aldons J Lusis,Jin J Zhou,Christopher S Seet,Donald B Kohn,Pin Wang,Xianghong Jasmine Zhou,Matteo Pellegrini,Benjamin R Puliafito,Sarah M Larson,Lili Yang
제품이 사용된 논문 모두 보기
style
left-column
style
left-column
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com