AB326501

PE Anti-IL-2 Receptor alpha antibody [MEM-181]

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Mouse Monoclonal IL2RA antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Human samples.

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Flow Cytometry - PE Anti-IL-2 Receptor alpha antibody [MEM-181] (AB326501)

Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-IL-2 Receptor alpha antibody [MEM-181] (AB326501)

Separation of CD3 positive CD25 positive lymphocytes (red-filled) from CD3 negative CD25 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human PHA stimulated peripheral whole blood stained using ab326501 (concentration in sample 0.5 μg/ml).

Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-IL-2 Receptor alpha antibody [MEM-181] (AB326501)

Flow cytometry multicolor surface staining pattern of human PHA stimulated lymphocytes using ab326501 (concentration in sample 0.5 μg/ml) and anti-human CD3 (UCHT1) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-IL-2 Receptor alpha antibody [MEM-181] (AB326501)

Flow cytometry surface staining pattern of human peripheral whole blood stained using ab326501 (concentration in sample 0.5 μg/ml).

Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-IL-2 Receptor alpha antibody [MEM-181] (AB326501)

Separation of CD4 positive CD25 positive Treg cells (red-filled) from CD4 negative CD25 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using ab326501 (concentration in sample 0.5 μg/ml).

Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-IL-2 Receptor alpha antibody [MEM-181] (AB326501)

Flow cytometry multicolor surface staining pattern of human lymphocytes using ab326501 (concentration in sample 0.5 μg/ml) and anti-human CD4 (MEM-241) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

MEM-181

Isotype

IgG1

Light chain 타입

kappa

Conjugation

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

Specificity

The antibody MEM-181 reacts with an extracellular epitope of CD25 (Interleukin-2 receptor alpha chain), a 55 kDa type I transmembrane glycoprotein expressed on activated B and T lymphocytes, activated monocytes/macrophages and on CD4+ T lymphocytes (T regulatory cells); it is lost on resting B and T lymphocytes.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.</p>" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Size-exclusion chromatography

Purification 관련 사항

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

보관 버퍼

pH: 7.4 Preservative: 0.098% Sodium azide Constituents: PBS, 0.2% BSA

배송 시 보관 조건

Ambient - Can Ship with Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

Do Not Freeze|Store in the dark

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Receptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.

See full target information IL2RA

대체 명칭 보기

CD25, Interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, TAC antigen, p55, IL2RA

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PE Anti-IL-2 Receptor alpha antibody [MEM-181]

Flow Cytometry - PE Anti-IL-2 Receptor alpha antibody [MEM-181] (AB326501)

Flow Cytometry - PE Anti-IL-2 Receptor alpha antibody [MEM-181] (AB326501)

Flow Cytometry - PE Anti-IL-2 Receptor alpha antibody [MEM-181] (AB326501)

Flow Cytometry - PE Anti-IL-2 Receptor alpha antibody [MEM-181] (AB326501)

Flow Cytometry - PE Anti-IL-2 Receptor alpha antibody [MEM-181] (AB326501)

관련 conjugated 항체와 다양한 조성의 항체 (7)

주요 정보

Host species

Clonality

Clone number

Isotype

Light chain 타입

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Specificity

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

제품 프로토콜

타겟 정보

대체 명칭 보기

Product promise