JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB213192

PE Anti-Insulin antibody [EPR17359]

Be the first to review this product! 리뷰 제출

|

(1 출판물)

Rabbit Recombinant Monoclonal Insulin antibody - conjugated to PE. Suitable for Flow Cyt (Intra), ICC/IF, IHC-P and reacts with Rat, Human, Mouse samples. Cited in 1 publication.

대체 명칭 보기

Insulin, INS

1 이미지
Flow Cytometry (Intracellular) - PE Anti-Insulin antibody [EPR17359] (AB213192)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - PE Anti-Insulin antibody [EPR17359] (AB213192)

Overlay histogram showing Rin5F cells stained with ab213192 (red line). The cells were fixed with 4% formaldehyde and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab213192, 1/1000 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 50mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

This antibody gave a positive signal in Rin5F cells fixed with 80% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

관련 conjugated 항체와 다양한 조성의 항체 (2)

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17359

Isotype

IgG

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Reacts with

Rat, Human, Mouse

Applications

ICC/IF, Flow Cyt (Intra), IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

대체품

Primary Antibodies

AB210560

Anti-Insulin antibody

Lab Essentials

IHC - Wholemount - Anti-Insulin antibody (AB210560)

  • 0
  • 0
Primary Antibodies

AB31906

Anti-Insulin Affibody® Molecule

Thin Layer Chromatography - Anti-Insulin Affibody® Molecule (AB31906)

  • 0
  • 0
Primary Antibodies

AB46707

Anti-Insulin antibody [IN-05] - Low endotoxin, Azide free

  • 0
  • 0
Primary Antibodies

AB9569

Anti-Insulin antibody [E2E3]

Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin antibody [E2E3] (AB9569)

  • 4
  • 2
Primary Antibodies

AB53591

Anti-Insulin antibody

Lab Essentials

ELISA - Anti-Insulin antibody (AB53591)

  • 0
  • 0
Primary Antibodies

AB6995

Anti-Insulin antibody [K36aC10]

Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin antibody [K36aC10] (AB6995)

  • 4
  • 9
Primary Antibodies

AB63820

Anti-Insulin antibody

BOND RX™ Validated|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin antibody (AB63820)

  • 4
  • 5
Primary Antibodies

AB133281

Anti-Insulin antibody [EPR3074]

20ul selling size|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin antibody [EPR3074] (AB133281)

  • 0
  • 1
Primary Antibodies

AB53592

Biotin Anti-Insulin antibody

ELISA - Biotin Anti-Insulin antibody (AB53592)

  • 0
  • 0

보완 제품

Assay Kits

AB65333

Glucose Assay Kit

Functional Studies - Glucose Assay Kit (AB65333)

  • 0
  • 0
Primary Antibodies

AB283689

Anti-Insulin Receptor alpha antibody [EPR23962-157]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin Receptor alpha antibody [EPR23962-157] (AB283689)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Assay Kits

AB273188

Bovine Insulin ELISA Kit

Sandwich ELISA - Bovine Insulin ELISA Kit (AB273188)

  • 0
  • 0
Primary Antibodies

AB92517

Anti-Glucagon antibody [EP3070]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] (AB92517)

  • 5
  • 8
style
left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/1000", "FlowCytIntra-species-notes": "<p><strong>The cellular localisation of this product has been verified in ICC/IF.</strong></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
style
left-column

제품 세부 정보

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
분주 정보
Upon delivery aliquot
보관 정보
Do Not Freeze|Store in the dark
style
left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Insulin a peptide hormone plays an essential mechanical role in carbohydrate and fat metabolism. Insulin alternatively known as bovine insulin in some research contexts has a molecular mass of around 5800 Da. Beta cells in the pancreas express insulin where it gets secreted directly into the bloodstream. The hormone regulates glucose levels by facilitating the uptake of glucose into cells such as muscle and adipose tissues.
Biological function summary

Insulin influences several key metabolic processes by binding to its receptor a component of the insulin receptor substrate family. This triggers a cascade of events within cells promoting the uptake of glucose and converting it into energy. Insulin operates as a singular entity but plays a critical role in forming a complex with its receptor. This interaction triggers downstream effects that alter the activity of enzymes and transcription factors involved in glucose and lipid metabolism.

Pathways

Insulin is critical for the regulation of the PI3K-AKT signaling pathway and the MAPK pathway. These pathways modulate processes such as cell growth proliferation and survival. Insulin interacts closely with other proteins in these pathways such as the insulin receptor substrate proteins and influences downstream proteins like AKT kinase which has a significant role in mediating the metabolic effects initiated by insulin binding.

Insulin is most notably linked to diabetes mellitus characterized by insufficient insulin production or action. This deficiency disrupts normal glucose homeostasis leading to hyperglycemia. Other associated proteins include glucagon which counteracts insulin's role in lowering blood glucose levels and IRS-1 an important element through which insulin signaling is transduced. Researchers utilize mouse insulin ELISA and insulin ELISA kits to better understand these relationships and develop treatments for insulin-related disorders.
style
left-column
style
left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

타겟 정보

Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
See full target information INS
style
left-column

제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of orthopaedic surgery and research 18:937 PubMed38062424

2023

Exosomal miR-182 derived from bone marrow mesenchymal stem cells drives carfilzomib resistance of multiple myeloma cells by targeting SOX6.

Applications

Unspecified application

Species

Unspecified reactive species

Shifeng Long,Shengping Long,Honglei He,Liang Luo,Mei Liu,Ting Ding
제품이 사용된 논문 모두 보기
style
left-column
style
left-column
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com