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AB27866

PE Anti-Interferon gamma antibody [CC302]

4

(1 리뷰)

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(2 제품이 사용된 논문 )

Anti-Interferon gamma antibody [CC302] conjugated to PE - suitable for flow cytometry, and reacts with human, cattle, dog, horse, and other samples. Clone [CC302] is a mouse monoclonal antibody cited in 83 publications.

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주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

CC302

Isotype

IgG1

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Reacts with

Sheep, Goat, Horse, Cow, Dog, Human, Pig, Ferret

Applications

Flow Cyt

applications

Specificity

This antibody recognises bovine interferon gamma

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "1/1 - 1/10", "FlowCyt-species-notes": "<p><a href='/ko/products/primary-antibodies/pe-mouse-igg1-b11-6-isotype-control-ab91357'>ab91357</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p><p>Neat - 1/10. Use 10μl of the suggested working dilution to label 10<sup>6</sup> cells in 100μl. Membrane permeabilisation is required for this application.</p>" }, "Cow": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "1/1 - 1/10", "FlowCyt-species-notes": "<p><a href='/ko/products/primary-antibodies/pe-mouse-igg1-b11-6-isotype-control-ab91357'>ab91357</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p><p>Neat - 1/10. Use 10μl of the suggested working dilution to label 10<sup>6</sup> cells in 100μl. Membrane permeabilisation is required for this application.</p>" }, "Dog": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "1/1 - 1/10", "FlowCyt-species-notes": "<p><a href='/ko/products/primary-antibodies/pe-mouse-igg1-b11-6-isotype-control-ab91357'>ab91357</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p><p>Neat - 1/10. Use 10μl of the suggested working dilution to label 10<sup>6</sup> cells in 100μl. Membrane permeabilisation is required for this application.</p>" }, "Ferret": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "1/1 - 1/10", "FlowCyt-species-notes": "<p><a href='/ko/products/primary-antibodies/pe-mouse-igg1-b11-6-isotype-control-ab91357'>ab91357</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p><p>Neat - 1/10. Use 10μl of the suggested working dilution to label 10<sup>6</sup> cells in 100μl. Membrane permeabilisation is required for this application.</p>" }, "Goat": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "1/1 - 1/10", "FlowCyt-species-notes": "<p><a href='/ko/products/primary-antibodies/pe-mouse-igg1-b11-6-isotype-control-ab91357'>ab91357</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p><p>Neat - 1/10. Use 10μl of the suggested working dilution to label 10<sup>6</sup> cells in 100μl. Membrane permeabilisation is required for this application.</p>" }, "Horse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "1/1 - 1/10", "FlowCyt-species-notes": "<p><a href='/ko/products/primary-antibodies/pe-mouse-igg1-b11-6-isotype-control-ab91357'>ab91357</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p><p>Neat - 1/10. Use 10μl of the suggested working dilution to label 10<sup>6</sup> cells in 100μl. Membrane permeabilisation is required for this application.</p>" }, "Pig": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "1/1 - 1/10", "FlowCyt-species-notes": "<p><a href='/ko/products/primary-antibodies/pe-mouse-igg1-b11-6-isotype-control-ab91357'>ab91357</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p><p>Neat - 1/10. Use 10μl of the suggested working dilution to label 10<sup>6</sup> cells in 100μl. Membrane permeabilisation is required for this application.</p>" }, "Sheep": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "1/1 - 1/10", "FlowCyt-species-notes": "<p><a href='/ko/products/primary-antibodies/pe-mouse-igg1-b11-6-isotype-control-ab91357'>ab91357</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p><p>Neat - 1/10. Use 10μl of the suggested working dilution to label 10<sup>6</sup> cells in 100μl. Membrane permeabilisation is required for this application.</p>" } } }

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein G
보관 버퍼
pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 1% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
Do Not Freeze

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Interferon gamma (IFN-γ) also known as type II interferon is a cytokine that plays an important role in immune response. IFN-γ has a molecular weight of about 17 kDa and is produced by T cells and natural killer (NK) cells. IFN-γ binds to the interferon gamma receptor initiating a signaling cascade that activates various genes involved in immune functions. It is expressed mainly in activated immune cells within lymphoid tissues and inflamed sites during immune responses.
Biological function summary

This cytokine is significant in promoting macrophage activation enhancing the antigen presentation process and boosting the antimicrobial activity of phagocytes. IFN-γ is not part of a larger protein complex but works as a homodimer in signal transduction. Its production heightens the Th1 immune response by stimulating the differentiation of naïve T cells into Th1 cells which is essential for effective cellular immunity.

Pathways

IFN-γ is integrally involved in the JAK-STAT signaling pathway alongside another critical cytokine Interleukin-12. This pathway further amplifies the immune response by regulating the expression of genes associated with cellular defense mechanisms. IFN-γ also interacts with the NF-kB pathway influencing inflammation and the activation of further immune responses. These interactions show a network of cooperativity with proteins like STAT1 and NF-kB essential for executing its biological roles.

IFN-γ is linked to autoimmune diseases such as rheumatoid arthritis and multiple sclerosis where its elevated levels can exacerbate inflammatory processes. It connects to other proteins like TNF-alpha in promoting the inflammatory cascade. Moreover lower levels of IFN-γ are associated with a heightened risk of infections like tuberculosis demonstrating its vital role in pathogen defense. Therefore understanding IFN-γ and its interactions can be key in developing therapeutic approaches against these conditions.

제품 프로토콜

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타겟 정보

Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation (PubMed : 16914093, PubMed : 8666937). Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation (PubMed : 8349687). Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription (PubMed : 16914093). Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits (PubMed : 8666937). In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading (PubMed : 8163024). Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference (PubMed : 11112687). Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL (PubMed : 7729559). Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation (By similarity).
See full target information IFNG

대체 명칭 보기

Interferon gamma, IFN-gamma, Immune interferon, IFNG

제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Viruses 13: PubMed34209093

2021

Universal Live-Attenuated Influenza Vaccine Candidates Expressing Multiple M2e Epitopes Protect Ferrets against a High-Dose Heterologous Virus Challenge.

Applications

Unspecified application

Species

Unspecified reactive species

Daria Mezhenskaya,Irina Isakova-Sivak,Victoria Matyushenko,Svetlana Donina,Andrey Rekstin,Konstantin Sivak,Kirill Yakovlev,Anastasia Katelnikova,Kirill Kryshen,Valery Makarov,Larisa Rudenko

Infection and immunity 73:3778-82 PubMed15908411

2005

Expression cloning of gamma interferon-inducing antigens of Mycobacterium avium subsp. paratuberculosis.

Applications

Unspecified application

Species

Unspecified reactive species

Reiko Nagata,Yoshihiro Muneta,Kazuhiro Yoshihara,Yuichi Yokomizo,Yasuyuki Mori
제품이 사용된 논문 모두 보기

Product promise

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