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AB278691

PE Anti-PI 3 Kinase p85 alpha (pY467) + PI3 Kinase p55 (pY199) antibody [PI3KY458-1A11]

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(3 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal PI 3 Kinase p85 alpha phospho Y467 antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human PIK3R1 pY467.

대체 명칭 보기

GRB1, PIK3R1, Phosphatidylinositol 3-kinase regulatory subunit alpha, PI3-kinase regulatory subunit alpha, PI3K regulatory subunit alpha, PtdIns-3-kinase regulatory subunit alpha, Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha, PI3-kinase subunit p85-alpha, PtdIns-3-kinase regulatory subunit p85-alpha, Phosphatidylinositol 3-kinase regulatory subunit gamma, PI3-kinase regulatory subunit gamma, PI3K regulatory subunit gamma, PtdIns-3-kinase regulatory subunit gamma, Phosphatidylinositol 3-kinase 55 kDa regulatory subunit gamma, p55PIK, PI3-kinase subunit p55-gamma, PtdIns-3-kinase regulatory subunit p55-gamma, PIK3R3

1 이미지
Flow Cytometry - PE Anti-PI 3 Kinase p85 alpha (pY467) + PI3 Kinase p55 (pY199) antibody [PI3KY458-1A11] (AB278691)
  • Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-PI 3 Kinase p85 alpha (pY467) + PI3 Kinase p55 (pY199) antibody [PI3KY458-1A11] (AB278691)

Flow cytometric analysis of Ramos cells unstained cells (blue) or untreated (red) or treated with pervanadate (green) using ab278691.

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

PI3KY458-1A11

Isotype

IgG

Light chain 타입

kappa

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Synthetic Peptide within Human PIK3R1 pY467. The exact immunogen used to generate this antibody is proprietary information.

P27986

Specificity

<p>Recent data suggests this antibody can recognize PI 3 Kinase p85 and p55&#46;</p>

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보완 제품

Primary Antibodies

AB235266

Anti-PI 3 Kinase p85 alpha (phospho Y607) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha (phospho Y607) antibody (AB235266)

  • 0
  • 0
Primary Antibodies

AB191606

Anti-PI 3 Kinase p85 alpha antibody [EPR18702]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p85 alpha antibody [EPR18702] (AB191606)

  • 5
  • 2
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB172730

Rabbit IgG, monoclonal [EPR25A] - Isotype Control

RabMAb|Advanced Validation|Recombinant

Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "5 µL for 10^6 Cells", "FlowCyt-species-notes": "<p></p>" } } }
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제품 세부 정보

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A/G
보관 버퍼
Preservative: 0.09% Sodium azide Constituents: PBS, 0.2% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
Store in the dark
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The PI 3 Kinase p85 alpha also known as PI3K p85 or PI3-kinase p85 is a regulatory subunit part of the phosphoinositide 3-kinases (PI3Ks) complex which is integral to cellular functions. The p85 alpha form partners with the catalytic subunit to form PI3K p55 known for its significant role in signaling. This subunit exhibits a molecular weight of approximately 85 kDa. It shows expression in various tissues including liver muscle and brain where it aids in cellular communication responding to extracellular signals.
Biological function summary

PI 3 Kinase p85 alpha plays an essential role in intracellular signaling where it functions as part of the PI3K complex. It functions to regulate metabolic processes by interacting with phosphatidylinositol lipids in the cell membrane. PI3K p55 form contributes to cell growth survival and proliferation often collaborating with the catalytic subunit p110 to execute these functions. The phospho PI3K form becomes active upon receiving external growth factors facilitating downstream signaling.

Pathways

The involvement of PI 3 Kinase p85 alpha extends to critical cellular pathways like the PI3K/AKT pathway and the insulin signaling pathway. It engages with proteins such as AKT (protein kinase B) and IRS-1 linking to various cellular functions including metabolism protein synthesis and cellular proliferation. The activity of PI3K p55 form regulates these pathways by influencing enzyme activity and pathways associated with cell survival and apoptosis.

Dysregulation of PI 3 Kinase p85 alpha associates closely with cancers and metabolic disorders like type 2 diabetes. Aberrations in the p55 form link to tumorigenesis due to its role in uncontrolled cell proliferation. PI3K pathway disorders also relate to insulin resistance where the malfunction of PI3K components disrupts normal glucose uptake and insulin signaling. This target when interacting with proteins like PTEN further influences the development of cancer highlighting its critical role in both oncogenic and metabolic pathologies.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling (PubMed : 17626883, PubMed : 19805105, PubMed : 7518429). Modulates the cellular response to ER stress by promoting nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (PubMed : 20348923).
See full target information PIK3R1 pY467
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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cancer cell international 25:122 PubMed40158101

2025

The m6A reader IGF2BP2 promotes pancreatic cancer progression through the m6A-SLC1A5-mTORC1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xi Pu,Yuting Wu,Weiguo Long,Xinyu Sun,Xiao Yuan,Deqiang Wang,Xu Wang,Min Xu

Physiology international 109:334-347 PubMed36067021

2022

NCAPH promotes proliferation as well as motility of breast cancer cells by activating the PI3K/AKT pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Zhang,Peng Li,Wanying Guo,Qipeng Liu,Weiqiang Qiao,Miao Deng

Bioengineered 12:10761-10770 PubMed34852709

2021

Tetraspanin 1 (TSPAN1) promotes growth and transferation of breast cancer cells via mediating PI3K/Akt pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yange Wu,Wenxiu Chen,Yufeng Gong,Hongxia Liu,Bo Zhang
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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