PE Anti-SLC34A2 antibody [SP322] - N-terminal

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - PE Anti-SLC34A2 antibody [SP322] - N-terminal (AB322453)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH : OVCAR-3 (human ovary adenocarcinoma epithelial cell) cells labelling SLC34A2 - N-terminal with ab322453 at 1/50 (10.0 ug/ml) dilution.

Confocal image showing cytoplasmic and membranous staining in NIH : OVCAR-3 cell line (shown in magenta). The counterstain was observed in green. Nuclear DNA was labelled with DAPI (shown in blue).
Negative control : HCT116 (PMID 18660501).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - PE Anti-SLC34A2 antibody [SP322] - N-terminal (AB322453)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HCT 116 (human colorectal carcinoma epithelial cell, Left) / NIH : OVCAR-3 (human ovary adenocarcinoma epithelial cell, Right) cells labelling SLC34A2 - N-terminal with ab322453 at 1/5000 dilution (0.01ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (ab209478) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Negative control : HCT 116 (PMID : 18660501)