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AB72480

PE Anti-V5 tag antibody

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(4 제품이 사용된 논문 )

Anti-V5 tag antibody conjugated to PE is a rabbit polyclonal antibody suitable for flow cytometry, sELISA.
2 이미지
Flow Cytometry - PE Anti-V5 tag antibody (AB72480)
  • Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-V5 tag antibody (AB72480)

Flow cytometry analysis of a recombinant (N-terminus V5-tag) transmembrane cell surface receptor in CHO cells using ab72480 (red). Black - Mouse IgG1 Phycoerythrin isotype control.

Sandwich ELISA - PE Anti-V5 tag antibody (AB72480)
  • sELISA

Unknown

Sandwich ELISA - PE Anti-V5 tag antibody (AB72480)

Curve generated from a streptavidin-coated 96-well plate probed with dilutions of biotinylated V5 tagged peptide, and read after a 2 hour incubation at room temperature with ab72480.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Applications

sELISA, Flow Cyt

applications

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/ko/products/primary-antibodies/pe-rabbit-fab2-igg-isotype-control-ab37409'>ab37409</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }

제품 세부 정보

What is this antibody validated in?
PE Anti-V5 tag antibody (ab72480) is a rabbit polyclonal antibody and is validated for use in Flow Cytometry (Flow Cyt), ELISA. Tagged protein detection
V5 tag antibodies are used to visualize proteins labelled with this tag in a variety of applications.

특성 및 보관 정보

제형
Liquid
Purification 관련 사항
Size exclusion chromatography as well as some proprietary methods for purifying the final materials were used.
보관 버퍼
pH: 7.4 Preservative: 0.013% Sodium azide Constituents: 1.71% Sucrose, 1.64% Sodium phosphate, 0.87% Sodium chloride, 0.1% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The V5 tag is an epitope tag derived from the P and V fusion proteins of the paramyxovirus. It typically measures around 14 amino acids in length and is often used in molecular biology for tagging and protein expression studies. In terms of mechanical function the V5 tag facilitates the detection characterization and purification of recombinant proteins. Scientists commonly use it in various hosts such as bacteria yeast and mammalian cells due to its convenient integration into larger protein complexes. Unlike native proteins it serves as a non-disruptive label for identifying proteins without affecting their functionality.
Biological function summary

Researchers employ the V5 tag to decipher protein localization expression and role within cellular environments. It allows easy tracking in situ by using antibodies that target the V5 epitope such as the anti-V5 antibody. The V5 tag proves effective in dual-tagging systems useful in elucidating protein interactions and complex formations for instance when combined with other tags like biotin tag or APC tag. This assists in isolating and identifying components of complex cellular machinery.

Pathways

The tag is frequently utilized in studies exploring functional pathways of various proteins across metabolic and signaling pathways. It aids in examining the relationship between proteins within signaling cascades by tagging proteins that interact with others such as those in the PK tag-related signaling pathways. Researchers often track proteins across intricate networks to understand cellular processes intricately using V5-tagged proteins as indicators or reporters.

Scientists harness the V5 tag to investigate the expression and interactions of proteins associated with certain conditions such as cancer and neurodegenerative diseases. Through its integration scientists aim to uncover alterations in protein interactions or localizations that contribute to disease progression. For example they may tag proteins linked to cancer pathways observing how disruptions in proteins related to anti-V5 targets could provide insights into tumor biology or pathogenesis.

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

타겟 정보

See full target information V5 tag

대체 명칭 보기

GKPIPNPLLGLDST epitope tag, GKPIPNPLLGLDST tag, Protein Rev, Regulator of expression of viral proteins, V5 epitope tag

제품이 사용된 논문 (4)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Viruses 15: PubMed37243140

2023

Anti-Schmallenberg Virus Activities of Type I/III Interferons-Induced Mx1 GTPases from Different Mammalian Species.

Applications

Unspecified application

Species

Unspecified reactive species

Calixte Bayrou,Anne-Sophie Van Laere,Phai Dam Van,Nassim Moula,Mutien-Marie Garigliany,Daniel Desmecht

Journal of inherited metabolic disease 45:169-182 PubMed34741542

2021

A defect in molybdenum cofactor binding causes an attenuated form of sulfite oxidase deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Alexander Tobias Kaczmarek,Daniel Bender,Titus Gehling,Joshua Benedict Kohl,Hülya-Sevcan Daimagüler,José Angel Santamaria-Araujo,Max Christoph Liebau,Anne Koy,Sebahattin Cirak,Guenter Schwarz

Journal of virology 94: PubMed31694951

2020

Intact Viral Particle Counts Measured by Flow Virometry Provide Insight into the Infectivity and Genome Packaging Efficiency of Moloney Murine Leukemia Virus.

Applications

Unspecified application

Species

Unspecified reactive species

Tyler Milston Renner,Vera A Tang,Dylan Burger,Marc-André Langlois

Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research 39:274-282 PubMed30939061

2019

Anti-Influenza A Virus Activities of Type I/III Interferons-Induced Mx1 GTPases from Different Mammalian Species.

Applications

Unspecified application

Species

Unspecified reactive species

Phai Dam Van,Daniel Desmecht,Mutien-Marie Garigliany,Dao Bui Tran Anh,Anne-Sophie Van Laere
제품이 사용된 논문 모두 보기

Product promise

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