- Flow Cyt
Supplier Data
Flow Cytometry - PE Anti-V5 tag antibody (AB72480)
Flow cytometry analysis of a recombinant (N-terminus V5-tag) transmembrane cell surface receptor in CHO cells using ab72480 (red). Black - Mouse IgG1 Phycoerythrin isotype control.
- sELISA
Unknown
Sandwich ELISA - PE Anti-V5 tag antibody (AB72480)
Curve generated from a streptavidin-coated 96-well plate probed with dilutions of biotinylated V5 tagged peptide, and read after a 2 hour incubation at room temperature with ab72480.
Reactivity 정보
제품 세부 정보
PE Anti-V5 tag antibody (ab72480) is a rabbit polyclonal antibody and is validated for use in Flow Cytometry (Flow Cyt), ELISA. Tagged protein detection
V5 tag antibodies are used to visualize proteins labelled with this tag in a variety of applications.
특성 및 보관 정보
제형
Purification 관련 사항
보관 버퍼
배송 시 보관 조건
적절한 단기 보관 조건
적절한 장기 보관 조건
추가 정보
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Researchers employ the V5 tag to decipher protein localization expression and role within cellular environments. It allows easy tracking in situ by using antibodies that target the V5 epitope such as the anti-V5 antibody. The V5 tag proves effective in dual-tagging systems useful in elucidating protein interactions and complex formations for instance when combined with other tags like biotin tag or APC tag. This assists in isolating and identifying components of complex cellular machinery.
Pathways
The tag is frequently utilized in studies exploring functional pathways of various proteins across metabolic and signaling pathways. It aids in examining the relationship between proteins within signaling cascades by tagging proteins that interact with others such as those in the PK tag-related signaling pathways. Researchers often track proteins across intricate networks to understand cellular processes intricately using V5-tagged proteins as indicators or reporters.
제품 프로토콜
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타겟 정보
대체 명칭 보기
제품이 사용된 논문 (4)
Recent publications for all applications. Explore the 전체 목록 and refine your search
Viruses 15: PubMed37243140
2023
Applications
Unspecified application
Species
Unspecified reactive species
Journal of inherited metabolic disease 45:169-182 PubMed34741542
2021
Applications
Unspecified application
Species
Unspecified reactive species
Journal of virology 94: PubMed31694951
2020
Applications
Unspecified application
Species
Unspecified reactive species
Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research 39:274-282 PubMed30939061
2019
Applications
Unspecified application
Species
Unspecified reactive species
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