AB211621

PE Anti-Ym-1 + Ym-2 antibody [EPR15263]

RabMAb
Recombinant
각 태그의 뜻

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(4 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal CHIL3 antibody - conjugated to PE. Suitable for ICC/IF, IHC-P and reacts with Mouse, Rat samples. Cited in 4 publications.

대체 명칭 보기

Chi3l3, Ym1, Chil3, Chitinase-like protein 3, Beta-N-acetylhexosaminidase Ym1, Chitinase-3-like protein 3, ECF-L, Eosinophil chemotactic cytokine, Secreted protein Ym1, Chi3l4, Ym2, Chil4, Chitinase-like protein 4, Chitinase-3-like protein 4, Secreted protein Ym2

1 이미지

Immunocytochemistry/ Immunofluorescence - PE Anti-Ym-1 + Ym-2 antibody [EPR15263] (AB211621)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - PE Anti-Ym-1 + Ym-2 antibody [EPR15263] (AB211621)

ab211621 staining Chitinase 3 like protein 3 + Chitinase 3 like protein 4 in RAW264.7 cells. The cells were fixed with 100% methanol, permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab211621 at 1/100 dilution (pseudocolored in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in RAW264.7 cells fixed with 4% formaldehyde.

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15263

Isotype

IgG

Conjugation

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

Reacts with

Mouse, Mouse, Rat

Applications

IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "This product gave a positive signal in RAW264.7 cells fixed with 100% methanol (5 min), and 4% formaldehyde.", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

분주 정보

Upon delivery aliquot

보관 정보

Do Not Freeze|Store in the dark

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Ym-1 and Ym-2 also known as chitinase-like proteins exhibit mechanical roles in the immune system without enzymatic chitinase activity. Ym-1 with a mass of approximately 43 kDa and Ym-2 are expressed predominantly in macrophages especially within inflammatory and allergic contexts. Expression is induced by IL-4 and IL-13 cytokines. Both proteins may also show presence in neutrophils and other innate immune cells during particular immune responses marking them important players in immune modulation.

Biological function summary

Ym-1 and Ym-2 regulate tissue repair and remodeling. They play an important role in cell proliferation and matrix formation which is actively involved in tissue homeostasis and wound healing. These proteins are not typically found as part of a larger complex but may interact with multiple signaling molecules in the cell's microenvironment. Their functions in shaping the extracellular matrix and facilitating cell migration make them significant in resolving inflammation.

Pathways

Ym-1 and Ym-2 are involved in the Th2 immune response and arginase pathways. They are associated with the regulation of Th2 cytokine production a pathway interconnected with adaptive immune responses to parasitic infections and allergic reactions. Ym proteins align with arginase pathways important in tissue repair and fibrosis and often correlate with Arginase-1 especially during inflammation and healing processes.

Ym-1 and Ym-2 connect predominately to asthma and fibrosis. Elevated levels observed in these conditions indicate their involvement in disease progression. In asthma Ym proteins modulate airway inflammation and remodeling collaborating with proteins like TGF-β to influence fibrosis and extracellular matrix deposition. The interaction between Ym proteins and such growth factors outlines their participation in pathological conditions offering potential targets for therapeutic interventions.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Lectin that binds saccharides with a free amino group, such as glucosamine or galactosamine. Binding to oligomeric saccharides is much stronger than binding to mono- or disaccharides. Also binds chitin and heparin. Has weak hexosaminidase activity but no chitinase activity. Has chemotactic activity for T-lymphocytes, bone marrow cells and eosinophils. May play a role in inflammation and allergy.

See full target information Chil3

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제품이 사용된 논문 (4)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Frontiers in pharmacology 15:1516111 PubMed39744137

2025

PM2.5-induced oxidative stress upregulates PLA2R expression in the lung and is involved in the pathogenesis of membranous nephropathy through extracellular vesicles.

Applications

Unspecified application

Species

Unspecified reactive species

Wang Zhang,Jiating Chen,Ye Yuan,Jiao Luo,Zhanmei Zhou,Guobao Wang

International forum of allergy & rhinology 13:230-241 PubMed35950767

2022

Chronic interleukin-13 expression in mouse olfactory mucosa results in regional aneuronal epithelium.

Applications

Unspecified application

Species

Unspecified reactive species

Anirudh Saraswathula,Melissa M Liu,Heather Kulaga,Andrew P Lane

Neuropathology : official journal of the Japanese Society of Neuropathology 42:181-189 PubMed35434787

2022

NLRP3 inflammasome inhibitor ameliorates ischemic stroke by reprogramming the phenotype of microglia/macrophage in a murine model of distal middle cerebral artery occlusion.

Applications

Unspecified application

Species

Unspecified reactive species

Hongning Jia,Xiaoyuan Qi,Lan Fu,Huijun Wu,Jinxing Shang,Mingwei Qu,Chaoping Yang,Jianping Wang

Scientific reports 9:7149 PubMed31073167

2019

Pharmacological Mobilization and Recruitment of Stem Cells in Rats Stops Abdominal Adhesions After Laparotomy.

Applications

Unspecified application

Species

Unspecified reactive species

Kenichi Iwasaki,Ali Reza Ahmadi,Le Qi,Melissa Chen,Wei Wang,Kenji Katsumata,Akihiko Tsuchida,James Burdick,Andrew M Cameron,Zhaoli Sun

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PE Anti-Ym-1 + Ym-2 antibody [EPR15263]

Immunocytochemistry/ Immunofluorescence - PE Anti-Ym-1 + Ym-2 antibody [EPR15263] (AB211621)

관련 conjugated 항체와 다양한 조성의 항체 (4)

주요 정보

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Sample Prep & Detection Kits

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (4)

Product promise