AB50123

Anti-Pepsinogen I antibody [7G3]

Name: Anti-Pepsinogen I antibody [7G3]
Brand: Abcam Limited
SKU: AB50123
Rating: 4 (3 reviews)

BOND RX™ Validated
각 태그의 뜻

(3 리뷰들)

(4 제품이 사용된 논문 )

Anti-Pepsinogen I antibody [7G3] (ab50123) is a mouse monoclonal antibody detecting Pepsinogen I in IHC-P. Suitable for Human.

- Trusted since 2007

1 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pepsinogen I antibody [7G3] (AB50123)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pepsinogen I antibody [7G3] (AB50123)

IHC image of ab50123 staining in human normal stomach formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab50123, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

7G3

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

IHC-P

applications

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }

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제품 세부 정보

What is this antibody validated in?
Anti-Pepsinogen I antibody [7G3] (ab50123) is a mouse monoclonal antibody and is validated for use in Immunohistochemistry (IHC-P) in Human samples.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 Preservative: 0.09% Sodium azide Constituents: PBS, 0.87% Sodium chloride

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Pepsinogen I often referred to simply as pepsinogen is a zymogen form of the digestive enzyme pepsin. It possesses a molecular mass of approximately 44 kDa. This inactive precursor is synthesized and secreted by the gastric chief cells located in the stomach lining. Once released into the gastric lumen pepsinogen undergoes conversion into the active enzyme pepsin when exposed to the acidic pH of the stomach which facilitates protein breakdown during digestion.

Biological function summary

Pepsinogen I serves as an important component in the proteolytic process within the digestive system. It is not a part of a larger protein complex but functions independently after its activation. The conversion to pepsin enables the digestion of dietary proteins into smaller peptides and amino acids which are essential for the absorption and utilization by the body. This activity is fundamental to maintaining efficient nutrient processing and absorption in the gastrointestinal tract.

Pathways

The digestive process involving pepsinogen I is closely tied to the gastric acid secretion pathway. The enzyme pepsin derived from pepsinogen plays an important role in this pathway by breaking down proteins within the acidic environment of the stomach. Additionally pepsinogen activation indirectly interacts with other enzymes like gastric lipase which aids in the digestion of fats highlighting the interconnectedness of digestive pathways that ensure the body efficiently utilizes nutrients.

Pepsinogen I levels may be associated with conditions like gastritis and peptic ulcer disease. Aberrant expression or secretion can serve as a marker for these gastric disorders where pepsinogen I through its converted form pepsin can be involved in the excessive breakdown of the stomach lining. Additionally its relationship with gastric-related proteins like mucins which protect against acidic damage further accentuates its significance in maintaining gastric health and the potential implications when balance is disrupted.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

See full target information Pepsinogen I

대체 명칭 보기

Pepsinogen, Pepsinogen-1, Pepsinogen-A

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제품이 사용된 논문 (4)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Translational cancer research 10:3053-3058 PubMed35116613

2022

A case report of endoscopic submucosal dissection for a new subtype of gastric adenoma: mixed fundic and pyloric mucosa type.

Applications

Unspecified application

Species

Unspecified reactive species

Liu Han,Yuyong Tan,Deliang Liu,Songqing Fan,Lei Shi,Chengbai Liang

Nature protocols 16:3802-3835 PubMed34215862

2021

CODEX multiplexed tissue imaging with DNA-conjugated antibodies.

Applications

mIHC

Species

Human

Sarah Black,Darci Phillips,John W Hickey,Julia Kennedy-Darling,Vishal G Venkataraaman,Nikolay Samusik,Yury Goltsev,Christian M Schürch,Garry P Nolan

Journal of oncology 2020:5701527 PubMed32411238

2020

Cell Survival Is Regulated via SOX9/BCL2L1 Axis in HCT-116 Colorectal Cancer Cell Line.

Applications

Unspecified application

Species

Unspecified reactive species

Erik Lizárraga-Verdugo,Erika Ruiz-García,César López-Camarillo,Mercedes Bermúdez,Mariana Avendaño-Félix,Rosalío Ramos-Payán,Geovanni Romero-Quintana,Alfredo Ayala-Ham,Carlos Villegas-Mercado,Carlos Pérez-Plasencia,Maribel Aguilar-Medina

Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research 30:171-88 PubMed20038200

2009

Identification of cellular genes induced in human cells after activation of the OAS/RNaseL pathway by vaccinia virus recombinants expressing these antiviral enzymes.

Applications

Unspecified application

Species

Unspecified reactive species

Elena Domingo-Gil,José Manuel González,Mariano Esteban

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Pepsinogen I antibody [7G3]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pepsinogen I antibody [7G3] (AB50123)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (4)

Product promise