Anti-Peptide YY/PYY antibody [EPR21011-6R]

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peptide YY/PYY antibody [EPR21011-6R] (AB325645)

Immunohistochemical analysis of paraffin-embedded Mouse ileum tissue labeling Peptide YY/PYY with ab325645 at 1/2000 (0.262 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on enteroendocrine cells in mouse ileum.

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peptide YY/PYY antibody [EPR21011-6R] (AB325645)

Immunohistochemical analysis of paraffin-embedded Mouse pancreas tissue labeling Peptide YY/PYY with ab325645 at 1/2000 (0.262 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in the mouse pancreatic islets.

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peptide YY/PYY antibody [EPR21011-6R] (AB325645)

Immunohistochemical analysis of paraffin-embedded Rat pancreas tissue labeling Peptide YY/PYY with ab325645 at 1/2000 (0.262 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in the rat pancreatic islets.

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peptide YY/PYY antibody [EPR21011-6R] (AB325645)

Immunohistochemical analysis of paraffin-embedded Rat ileum tissue labeling Peptide YY/PYY with ab325645 at 1/2000 (0.262 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on enteroendocrine cells in rat ileum.

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Peptide YY/PYY antibody [EPR21011-6R] (AB325645)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse ileum tissue staining Peptide YY/PYY with ab325645 at a 1 : 2000 (0.262 µg/ml) dilution, ab111125 anti-GLP-1 used at 1 : 10000 (0.1 µg/ml) dilution.

Panel A : anti-Peptide YY (green; Opal™520), anti-GLP-1 (magenta; Opal™570) on mouse ileum.
Panel B : anti-Peptide YY staining enteroendocrine L cells in mouse ileum.
Panel C : anti-GLP-1 staining enteroendocrine L cells in mouse ileum.
Panel D : Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab325645 and ab111125 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Nuclear counter stain with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• WB

Lab

Western blot - Anti-Peptide YY/PYY antibody [EPR21011-6R] (AB325645)

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) loading control staining at 1/5000 and anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Peptide YY/PYY antibody [EPR21011-6R] (ab325645) at 1/1000 dilution

Lanes 1 - 2:

293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a myc-His-tag® whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a rat PYY expression vector containing a myc-His-tag® whole cell lysate at 20 µg

Observed band size: 15 kDa,17 kDa,36 kDa

false

Exposure time: 180s

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peptide YY/PYY antibody [EPR21011-6R] (AB325645)

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Peptide YY/PYY with ab325645 at 1/2000 (0.262 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining in the mouse liver.

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peptide YY/PYY antibody [EPR21011-6R] (AB325645)

Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling Peptide YY/PYY with ab325645 at 1/2000 (0.262 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining in the rat liver.

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• Dot

Lab

Dot Blot - Anti-Peptide YY/PYY antibody [EPR21011-6R] (AB325645)

Dot blot analysis of Peptide YY/PYY using ab325645 at 1 : 1000 (0.524 µg/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Lane 1 : Mouse PYY peptide

Lane 2 : Rat PYY peptide

Lane 3 : Mouse PPY peptide

Lane 4 : Mouse NPY peptide

Exposure time : 180 seconds.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with mouse PPY peptide or NPY peptide by dot blot.

All lanes:

Dot Blot - Anti-Peptide YY/PYY antibody [EPR21011-6R] (ab325645) at 1/1000 dilution

Lane 1:

Mouse PYY peptide

Lane 2:

Rat PYY peptide

Lane 3:

Mouse PPY peptide

Lane 4:

Mouse NPY peptide

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

true

Exposure time: 180s