AB114860

PerCP Anti-CD20 antibody [2H7]

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Anti-CD20 antibody [2H7] conjugated to PerCP - suitable for flow cytometry, and reacts with human samples. Clone [2H7] is a mouse monoclonal antibody and one of the most commonly used clones for flow cytometry.

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Flow Cytometry - PerCP Anti-CD20 antibody [2H7] (AB114860)

Flow Cyt

Supplier Data

Flow Cytometry - PerCP Anti-CD20 antibody [2H7] (AB114860)

Surface staining of human peripheral blood with ab114860.

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

2H7

Isotype

IgG2b

Conjugation

PerCP

Excitation/Emission

Ex: 490nm, Em: 675nm

Carrier free

Reacts with

Human, Primates

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing MS4A1 protein. The exact immunogen used to generate this antibody is proprietary information.

P11836

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대체품

Primary Antibodies

AB166865

Anti-CD20 antibody [EP459Y] - Low endotoxin, Azide free

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [EP459Y] - Low endotoxin, Azide free (AB166865)

Primary Antibodies

AB198941

Alexa Fluor® 488 Anti-CD20 antibody [EP459Y]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-CD20 antibody [EP459Y] (AB198941)

Primary Antibodies

AB198943

Alexa Fluor® 647 Anti-CD20 antibody [EP459Y]

Recombinant|Advanced Validation|RabMAb

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-CD20 antibody [EP459Y] (AB198943)

Primary Antibodies

AB236434

Anti-CD20 antibody [SP32] - BSA and Azide free

BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant

Multiplex immunohistochemistry - Anti-CD20 antibody [SP32] - BSA and Azide free (AB236434)

Primary Antibodies

AB64088

Anti-CD20 antibody [SP32]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [SP32] (AB64088)

Primary Antibodies

AB78237

Anti-CD20 antibody [EP459Y]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [EP459Y] (AB78237)

보완 제품

Assay Kits

AB285273

Human CD20 ELISA Kit

Sandwich ELISA - Human CD20 ELISA Kit (AB285273)

Primary Antibodies

AB305048

Alexa Fluor® 647 Anti-CD8 alpha antibody [CAL66]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD8 alpha antibody [CAL66] (AB305048)

Primary Antibodies

AB196147

Alexa Fluor® 647 Anti-CD4 antibody [EPR6855]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD4 antibody [EPR6855] (AB196147)

Primary Antibodies

AB196515

Alexa Fluor® 647 Anti-CD19 antibody [EPR5906]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-CD19 antibody [EPR5906] (AB196515)

Primary Antibodies

AB302698

Alexa Fluor® 647 Anti-CD14 antibody [SP192]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD14 antibody [SP192] (AB302698)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "10 µL for 10^6 Cells", "FlowCyt-species-notes": "(or 100 μl of whole blood).<a href='/ko/products/primary-antibodies/percp-mouse-igg2b-kappa-monoclonal-mpc-11-isotype-control-ab172890'>ab172890</a> - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody." }, "Primates": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "10 µL for 10^6 Cells", "FlowCyt-species-notes": "(or 100 μl of whole blood).<a href='/ko/products/primary-antibodies/percp-mouse-igg2b-kappa-monoclonal-mpc-11-isotype-control-ab172890'>ab172890</a> - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody." } } }

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제품 세부 정보

The mouse monoclonal antibody 2H7 recognizes CD20 (B1, Bp35), a 33-37 kDa non-glycosylated membrane receptor with four transmembrane domains, expressed on pre-B lymphocytes, resting and activated B cells (not plasma cells), follicular dendritic cells, and at low levels on peripheral blood T lymphocytes.

Purified antibody is conjugated with Peridinin-chlorophyll-protein complex (PerCP) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Size-exclusion chromatography

보관 버퍼

pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS, 0.2% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

CD20 also known as MS4A1 or L26 is a membrane-spanning 4-domains subfamily A member 1 protein. It is a non-glycosylated phosphoprotein with a molecular mass of approximately 33-37 kDa. Expressed extensively on the surface of B-cells CD20 plays an important role in B-cell activation and regulation. While CD20 is absent on early pro-B cells and plasma cells its presence increases as B-cells mature. Anti-CD20 antibodies such as 2H7 are commonly used in research and treatment to deplete B-cells due to the target's consistent expression in most stages of B-cell development.

Biological function summary

CD20 contributes significantly to calcium ion transport which is essential for the activation process of B-cells. Although CD20 does not belong to a larger protein complex its role centers around forming a calcium channel that allows a sustained influx of calcium into the B-cells. This influx is important for the signaling pathways that govern B-cell activation proliferation and differentiation impacting the immune response efficacy.

Pathways

CD20 is closely involved in the B-cell receptor (BCR) signaling pathway and the Fc gamma R-mediated phagocytosis pathway. These pathways play vital roles in adaptive immunity and immune response modulation. CD20's interaction with proteins like PI3K during BCR signaling enhances receptor-mediated cellular signals subsequently influencing downstream effectors involved in cell growth and survival of B-cells.

CD20 is strongly linked to certain blood cancers and autoimmune diseases including non-Hodgkin's lymphoma and rheumatoid arthritis. These conditions often exploit aberrant B-cell activity or count. CD20's expression on malignant B-cells in non-Hodgkin's lymphoma makes it an effective target for monoclonal antibody therapies such as rituximab an anti-CD20 antibody. Additionally CD20-targeted therapies can help deplete pathogenic B-cells in rheumatoid arthritis highlighting the protein's relevance in disease treatment.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes (PubMed : 12920111, PubMed : 3925015, PubMed : 7684739). Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR (PubMed : 12920111, PubMed : 18474602, PubMed : 7684739).

See full target information MS4A1

대체 명칭 보기

CD20, MS4A1, B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

The Journal of biological chemistry 273:344-8 PubMed9417086

1998

Rapid redistribution of CD20 to a low density detergent-insoluble membrane compartment.

Applications

Unspecified application

Species

Unspecified reactive species

J P Deans,S M Robbins,M J Polyak,J A Savage

The Journal of biological chemistry 270:22632-8 PubMed7545683

1995

Association of 75/80-kDa phosphoproteins and the tyrosine kinases Lyn, Fyn, and Lck with the B cell molecule CD20. Evidence against involvement of the cytoplasmic regions of CD20.

Applications

Unspecified application

Species

Unspecified reactive species

J P Deans,L Kalt,J A Ledbetter,G L Schieven,J B Bolen,P Johnson

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PerCP Anti-CD20 antibody [2H7]

Flow Cytometry - PerCP Anti-CD20 antibody [2H7] (AB114860)

관련 conjugated 항체와 다양한 조성의 항체 (13)

주요 정보

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (2)

Product promise