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AB157317

PerCP/Cy5.5® Anti-CD3 antibody [UCHT1]

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(5 제품이 사용된 논문 )

Anti-CD3 antibody [UCHT1] conjugated to PerCP/Cy5.5® - suitable for flow cytometry, intracellular flow cytometry, and reacts with human samples. Clone [UCHT1] is a mouse monoclonal antibody and one of the most commonly used clones for flow cytometry.

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Flow Cytometry - PerCP/Cy5.5® Anti-CD3 antibody [UCHT1] (AB157317)
  • Flow Cyt

Lab

Flow Cytometry - PerCP/Cy5.5® Anti-CD3 antibody [UCHT1] (AB157317)

Analysis by flow cytometry of ab157317 staining CD3 in human peripheral blood cells.

관련 conjugated 항체와 다양한 조성의 항체 (13)

  • 660 APC

    APC Anti-CD3 antibody [UCHT1]

  • 785 APC/Cy7®

    APC/Cy7® Anti-CD3 antibody [UCHT1]

  • Biotin

    Biotin Anti-CD3 antibody [UCHT1]

  • 519 FITC

    FITC Anti-CD3 epsilon antibody [UCHT1]

  • 519 FITC

    FITC Anti-CD3 antibody [UCHT1]

  • 578 PE

    PE Anti-CD3 epsilon antibody [UCHT1]

  • 667 PE/Cy5®

    PE/Cy5® Anti-CD3 antibody [UCHT1]

  • 778 PE/Cy7®

    PE/Cy7® Anti-CD3 antibody [UCHT1]

  • 675 PerCP

    PerCP Anti-CD3 antibody [UCHT1]

  • 387 mFluor™ UV375

    mFluor™ UV375 Anti-CD3e antibody [UCHT1]

  • 454 mFluor™ Violet 450

    mFluor™ Violet 450 Anti-CD3e antibody [UCHT1]

  • 704 redFluor™ 710

    redFluor™ 710 Anti-CD3 antibody [UCHT1]

  • 450 violetFluor™ 450

    violetFluor™ 450 Anti-CD3 antibody [UCHT1]

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

UCHT1

Isotype

IgG1

Conjugation

PerCP/Cy5.5®

Excitation/Emission

Ex: 482nm, Em: 695nm

Carrier free

No

Reacts with

Human, Primates

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing CD3D protein. The exact immunogen used to generate this antibody is proprietary information.

P04234

Specificity

ab157317 reacts with the epsilon chain of the CD3 complex.

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대체품

Primary Antibodies

AB135372

Anti-CD3 antibody [SP162]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [SP162] (AB135372)

  • 4
  • 10
Primary Antibodies

AB16044

Anti-CD3 antibody

Immunoprecipitation - Anti-CD3 antibody (AB16044)

  • 4
  • 2
Primary Antibodies

AB16669

Anti-CD3 epsilon antibody [SP7]

BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant|Lab Essentials|20ul selling size

Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [SP7] (AB16669)

  • 4
  • 80
Primary Antibodies

AB21703

Anti-CD3 epsilon antibody [SP7], prediluted

BOND RX™ Validated|RabMAb|Lab Essentials|Advanced Validation|Recombinant

Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [SP7], prediluted (AB21703)

  • 5
  • 1
Primary Antibodies

AB245731

Anti-CD3 antibody [SP162] - BSA and Azide free

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-CD3 antibody [SP162] - BSA and Azide free (AB245731)

  • 5
  • 3
Primary Antibodies

AB246799

Anti-CD3 antibody [SP162] - Low endotoxin, Azide free

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-CD3 antibody [SP162] - Low endotoxin, Azide free (AB246799)

  • 0
  • 0
Primary Antibodies

AB5690

Anti-CD3 epsilon antibody

BOND RX™ Validated|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody (AB5690)

  • 4
  • 46

보완 제품

Primary Antibodies

AB305048

Alexa Fluor® 647 Anti-CD8 alpha antibody [CAL66]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD8 alpha antibody [CAL66] (AB305048)

  • 5
  • 1
Primary Antibodies

AB200315

Alexa Fluor® 488 Anti-CD45 antibody [EP322Y]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-CD45 antibody [EP322Y] (AB200315)

  • 0
  • 0
Primary Antibodies

AB196147

Alexa Fluor® 647 Anti-CD4 antibody [EPR6855]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD4 antibody [EPR6855] (AB196147)

  • 5
  • 1
Primary Antibodies

AB237456

Alexa Fluor® 647 Anti-NCAM1 antibody [EP2567Y]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-NCAM1 antibody [EP2567Y] (AB237456)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "4 µL for 10^6 Cells", "FlowCyt-species-notes": "<p>(or 100 μl of whole blood).</p><p><a href='/ko/products/primary-antibodies/percp-cy55-mouse-igg1-kappa-monoclonal-mopc-21-isotype-control-ab157226'>ab157226</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" }, "Primates": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "4 µL for 10^6 Cells", "FlowCyt-species-notes": "<p>(or 100 μl of whole blood).</p><p><a href='/ko/products/primary-antibodies/percp-cy55-mouse-igg1-kappa-monoclonal-mopc-21-isotype-control-ab157226'>ab157226</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }
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제품 세부 정보

Purified antibody is conjugated with tandem dye PerCP/Cy5.5® under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Size-exclusion chromatography
보관 버퍼
pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS, 0.2% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD247/CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed : 2470098). In addition of this role of signal transduction in T-cell activation, CD3D plays an essential role in thymocyte differentiation. Indeed, participates in correct intracellular TCR-CD3 complex assembly and surface expression. In absence of a functional TCR-CD3 complex, thymocytes are unable to differentiate properly. Interacts with CD4 and CD8 and thus serves to establish a functional link between the TCR and coreceptors CD4 and CD8, which is needed for activation and positive selection of CD4 or CD8 T-cells (PubMed : 12215456).
See full target information CD3D

대체 명칭 보기

CD3d, T3D, CD3D, T-cell surface glycoprotein CD3 delta chain, T-cell receptor T3 delta chain
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제품이 사용된 논문 (5)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Translational oncology 40:101831 PubMed38039947

2023

Silencing of PD-1 combined with EBV-specific killer T cells for the treatment of EBV-associated B lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaping Wang,Zhijuan Xu,Yanli Lai,Yanli Zhang,Ping Zhang,Qitian Mu,Shujun Yang,Yongcheng Sun,Lixia Sheng,Guifang Ouyang

The Journal of experimental medicine 204:2537-44 PubMed17923503

2007

Different composition of the human and the mouse gammadelta T cell receptor explains different phenotypes of CD3gamma and CD3delta immunodeficiencies.

Applications

Unspecified application

Species

Unspecified reactive species

Gabrielle M Siegers,Mahima Swamy,Edgar Fernández-Malavé,Susana Minguet,Sylvia Rathmann,Alberto C Guardo,Verónica Pérez-Flores,Jose R Regueiro,Balbino Alarcón,Paul Fisch,Wolfgang W A Schamel

The New England journal of medicine 354:1913-21 PubMed16672702

2006

Inherited and somatic CD3zeta mutations in a patient with T-cell deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Frédéric Rieux-Laucat,Claire Hivroz,Annick Lim,Véronique Mateo,Isabelle Pellier,Françoise Selz,Alain Fischer,Françoise Le Deist

Proceedings of the National Academy of Sciences of 101:16268-73 PubMed15534202

2004

Crystal structure of a human CD3-epsilon/delta dimer in complex with a UCHT1 single-chain antibody fragment.

Applications

Unspecified application

Species

Unspecified reactive species

Kelly L Arnett,Stephen C Harrison,Don C Wiley

Journal of immunology (Baltimore, Md. : 1950) 161:1113-22 PubMed9686569

1998

Serine 16 of stathmin as a cytosolic target for Ca2+/calmodulin-dependent kinase II after CD2 triggering of human T lymphocytes.

Applications

Unspecified application

Species

Unspecified reactive species

S le Gouvello,V Manceau,A Sobel
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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