Anti-Periostin antibody [EPR19934] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR19934] - BSA and Azide free (AB223194)

This data was developed using ab219056, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Periostin with ab219056 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Extracellular matrix staining on human colon (PMID : 26023718).

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR19934] - BSA and Azide free (AB223194)

This data was developed using ab219056, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue labeling Periostin with ab219056 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Extracellular matrix staining on human lung cancer (PMID : 24273600).

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IP

Supplier Data

Immunoprecipitation - Anti-Periostin antibody [EPR19934] - BSA and Azide free (AB223194)

This data was developed using ab219056, the same antibody clone in a different buffer formulation.

Periostin was immunoprecipitated from 0.35 mg of Human breast cancer lysate with ab219056 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab219056 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : Human breast cancer lysate 10 μg (Input).
Lane 2 : ab219056 IP in Human breast cancer lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab219056 in Human breast cancer lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 30 seconds.

All lanes:

Immunoprecipitation - Anti-Periostin antibody [EPR19934] (<a href='/ko/products/primary-antibodies/periostin-antibody-epr19934-ab219056'>ab219056</a>)

Predicted band size: 93 kDa

false

• WB

Supplier Data

Western blot - Anti-Periostin antibody [EPR19934] - BSA and Azide free (AB223194)

This data was developed using ab219056, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Periostin antibody [EPR19934] (<a href='/ko/products/primary-antibodies/periostin-antibody-epr19934-ab219056'>ab219056</a>) at 1/1000 dilution

All lanes:

Human breast cancer lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 93 kDa

Observed band size: 93 kDa

false

Exposure time: 3min

• WB

Supplier Data

Western blot - Anti-Periostin antibody [EPR19934] - BSA and Azide free (AB223194)

This data was developed using ab219056, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression tissues : liver, pancreas (PMID : 15514205, PMID : 29163770).

This antibody is not suitable for cell line testing.

All lanes:

Western blot - Anti-Periostin antibody [EPR19934] (<a href='/ko/products/primary-antibodies/periostin-antibody-epr19934-ab219056'>ab219056</a>) at 1/1000 dilution

Lane 1:

Human colon tissue lysate at 20 µg

Lane 2:

Human liver tissue lysate at 20 µg

Lane 3:

Human pancreas tissue lysate at 20 µg

Lane 4:

A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 5:

MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 6:

MDA-MB-468 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 7:

MG-63 (human osteosarcoma fibroblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 93 kDa

Observed band size: 93 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-Periostin antibody [EPR19934] - BSA and Azide free (AB223194)

This data was developed using ab219056, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Periostin antibody [EPR19934] (<a href='/ko/products/primary-antibodies/periostin-antibody-epr19934-ab219056'>ab219056</a>) at 1/1000 dilution

Lane 1:

Human fetal heart tissue lysate at 10 µg

Lane 2:

Human fetal kidney tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 93 kDa

Observed band size: 93 kDa

false

Exposure time: 3min