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AB215199

Anti-Periostin antibody [EPR20806]

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(25 제품이 사용된 논문 )

Anti-Periostin antibody [EPR20806] (ab215199) is a rabbit monoclonal antibody detecting Periostin in IHC-P, IHC-Fr. Suitable for Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency
- Over 10 publications

대체 명칭 보기

OSF2, POSTN, Periostin, PN, Osteoblast-specific factor 2, OSF-2

9 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR20806] (AB215199)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR20806] (AB215199)

Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling Periostin with ab215199 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive extracellular matrix staining on the stroma of human stomach (PMID : 15731169). Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR20806] (AB215199)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR20806] (AB215199)

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling Periostin with ab215199 at 1/1000 dilution (0.516 μg/ml) followed by a Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880) at a Ready to use dilution. Positive staining on extracellular matrix of human breast carcinoma. The section was incubated with ab215199 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880) at Ready to use dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

Immunohistochemistry (Frozen sections) - Anti-Periostin antibody [EPR20806] (AB215199)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Periostin antibody [EPR20806] (AB215199)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized mouse stomach tissue labeling Periostin with ab215199 at 1/500 dilution ,followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive cytoplasmic staining in the gastric lamina propia mucosae, negative staining in the epithelial cells (PMID : 24418260).

The nuclear counter stain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR20806] (AB215199)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR20806] (AB215199)

Immunohistochemical analysis of paraffin-embedded (A) Stomach tissue from wild-type C57BL/6JGpt mice, and (B) Stomach tissue from Postn knockout mice labeling Periostin with ab215199 at 1/10000 dilution.

Positive staining on (A) Stomach tissue from wild-type C57BL/6JGpt mice and no staining on (B) Stomach tissue from Postn knockout mice.

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and Postn-KO homozygous mice (Strain ID : T011526).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR20806] (AB215199)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR20806] (AB215199)

Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling Periostin with ab215199 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive extracellular matrix staining on the stroma of mouse colon (PMID : 15731169, PMID : 18443362). Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR20806] (AB215199)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR20806] (AB215199)

Immunohistochemical analysis of paraffin-embedded rat stomach tissue labeling Periostin with ab215199 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive extracellular matrix staining on the stroma of rat stomach (PMID : 15731169). Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Frozen sections) - Anti-Periostin antibody [EPR20806] (AB215199)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Periostin antibody [EPR20806] (AB215199)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized rat stomach tissue labeling Periostin with ab215199 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive cytoplasmic staining in the gastric lamina propia mucosae, negative staining in the epithelial cells (PMID : 24418260).

The nuclear counter stain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR20806] (AB215199)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR20806] (AB215199)

Immunohistochemical analysis of paraffin-embedded (A) Colon tissue from wild-type C57BL/6JGpt mice, and (B) Colon tissue from Postn knockout mice labeling Periostin with ab215199 at 1/10000 dilution.

Positive staining on (A) Colon tissue from wild-type C57BL/6JGpt mice and no staining on (B) Colon tissue from Postn knockout mice.

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and Postn-KO homozygous mice (Strain ID : T011526).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Western blot - Anti-Periostin antibody [EPR20806] (AB215199)
  • WB

Lab

Western blot - Anti-Periostin antibody [EPR20806] (AB215199)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200,000 dilution.

The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and Postn-KO homozygous mice (Strain ID : T011526).

All lanes:

Western blot - Anti-Periostin antibody [EPR20806] (ab215199) at 1/1000 dilution

Lane 1:

Wild-type mouse stomach tissue lysate (female case1) at 20 µg

Lane 2:

Wild-type mouse stomach tissue lysate (female case2) at 20 µg

Lane 3:

Postn knockout mouse stomach tissue lysate (female case1) at 20 µg

Lane 4:

Postn knockout mouse stomach tissue lysate (female case2) at 20 µg

Lane 5:

Wild-type mouse colon tissue lysate (female case1) at 20 µg

Lane 6:

Wild-type mouse colon tissue lysate (female case2) at 20 µg

Lane 7:

Postn knockout mouse colon tissue lysate (female case1) at 20 µg

Lane 8:

Postn knockout mouse colon tissue lysate (female case2 at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 75-95 kDa,36 kDa

false

Exposure time: 180s

관련 conjugated 항체와 다양한 조성의 항체 (3)

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20806

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-Fr, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/500", "IHCFr-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/10000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/500", "IHCFr-species-notes": "<p>Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

제품 세부 정보

What is this antibody validated in?
Anti-Periostin antibody [EPR20806] (ab215199) is a rabbit recombinant monoclonal antibody and is validated for use in Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr) in Human, Mouse, Rat samples.

Trusted by the scientific community
Anti-Periostin [EPR20806] (ab215199) was first used in a scientific publication in 2017 and has been cited over 10 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR20806] also available for your convenience: ab215199, Carrier free - ab227049, Alexa Fluor® 555 - ab307126

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
배송 시 보관 조건
Conditional Ambient
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Periostin also known as POSTN or OSF-2 is an extracellular matrix protein with a molecular weight of approximately 90 kDa. It plays a mechanical role in tissue remodeling by binding to integrins which supports tissue repair and regeneration. Periostin is mainly expressed in collagen-rich tissues such as bone periodontal ligament and cardiac tissues. It is synthesized by osteoblasts fibroblasts and other stromal cells functioning as a structural protein that supports cell adhesion and migration.
Biological function summary

The periostin protein contributes to the maintenance of tissue integrity and mechanical properties. It does not solely act; it forms part of a multiprotein complex with collagen and other extracellular matrix components. This involvement is necessary for maintaining cellular interactions and promoting tissue healing processes. Additionally periostin influences cell proliferation and migration impacting developmental and wound healing biology.

Pathways

Periostin is involved in the integrin signaling pathway and the Wnt/Β-catenin pathway both important for cell communication and movement. Through these pathways periostin interacts with integrins αvΒ3 and αvΒ5 facilitating cell adhesion and migration. The Wnt/Β-catenin pathway interaction involves several proteins including Frizzled receptors ensuring regulation of cellular proliferation and differentiation. These pathways make periostin a coordinator of complex cellular processes.

The periostin protein is associated with conditions such as fibrotic diseases and cardiovascular disorders. In fibrosis periostin promotes collagen cross-linking exacerbating tissue stiffness. This involves increased interaction with proteins like collagen types I and V contributing to pathological fibrotic progression. In cardiovascular disorders elevated periostin levels after myocardial injury are linked with fibrotic scarring where it collaborates with matrix metalloproteinases to remodel the cardiac tissue architecture. Understanding these connections can lead to novel therapeutic strategies.

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

타겟 정보

Induces cell attachment and spreading and plays a role in cell adhesion (PubMed : 12235007). Enhances incorporation of BMP1 in the fibronectin matrix of connective tissues, and subsequent proteolytic activation of lysyl oxidase LOX (By similarity).
See full target information POSTN

제품이 사용된 논문 (25)

Recent publications for all applications. Explore the 전체 목록 and refine your search

TH open : companion journal to thrombosis and haemostasis 9:a27014242 PubMed41059381

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Species

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Cell communication and signaling : CCS 23:371 PubMed40796869

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Xing Ji,Jiahao Luo,Yangxun He,Xinhua Hu,Taotao Xu,Yuanlong Wang,Sijun Pan,Jiali Yao,Weiwei Hu,Ximei Wu

Nature communications 16:3430 PubMed40210617

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Elias Glaros,Jonathan Foot,Ben Rayner,Heidi Schilter,Yunjia Zhang,Martina Paumann-Page,Mauro M Teixeira,Wolfgang Jarolimek,Shane R Thomas

Science advances 11:eadr7208 PubMed40203118

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Cancer communications (London, England) 45:172-177 PubMed39654335

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Journal for immunotherapy of cancer 12: PubMed39067872

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POSTN cancer-associated fibroblasts determine the efficacy of immunotherapy in hepatocellular carcinoma.

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Hao Wang,Yuan Liang,Zheng Liu,Rui Zhang,Jiashuo Chao,Mingming Wang,Mu Liu,Lei Qiao,Zhengfeng Xuan,Haitao Zhao,Ling Lu

Molecular cancer 23:120 PubMed38831402

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Species

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Xiaohang Qiao,Sabina Y van der Zanden,Xiaoyang Li,Minkang Tan,Yunxiang Zhang,Ji-Ying Song,Merle A van Gelder,Feija L Hamoen,Lennert Janssen,Charlotte L Zuur,Baoxu Pang,Olaf van Tellingen,Junmin Li,Jacques Neefjes

Translational pediatrics 13:596-609 PubMed38715675

2024

Characterization of the malignant cells and microenvironment of infantile fibrosarcoma via single-cell RNA sequencing.

Applications

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Species

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Yi Li,Qingchi Zhang,Ran Yang,Yong Zhan,Zifeng Li,Shuyang Dai,Deqian Chen,Lian Chen,Antonio Ruggiero,Chunjing Ye,Yifei Lu,Enqing Zhou,Rui Dong,Kuiran Dong

Nature communications 15:363 PubMed38191471

2024

Distinct mesenchymal cell states mediate prostate cancer progression.

Applications

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Species

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Hubert Pakula,Mohamed Omar,Ryan Carelli,Filippo Pederzoli,Giuseppe Nicolò Fanelli,Tania Pannellini,Fabio Socciarelli,Lucie Van Emmenis,Silvia Rodrigues,Caroline Fidalgo-Ribeiro,Pier Vitale Nuzzo,Nicholas J Brady,Wikum Dinalankara,Madhavi Jere,Itzel Valencia,Christopher Saladino,Jason Stone,Caitlin Unkenholz,Richard Garner,Mohammad K Alexanderani,Francesca Khani,Francisca Nunes de Almeida,Cory Abate-Shen,Matthew B Greenblatt,David S Rickman,Christopher E Barbieri,Brian D Robinson,Luigi Marchionni,Massimo Loda
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