Anti-Periostin antibody [EPR20806]
- RabMAb
- Recombinant
- 20ul selling size
- KO Validated
- 각 태그의 뜻
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(25 제품이 사용된 논문 )
Anti-Periostin antibody [EPR20806] (ab215199) is a rabbit monoclonal antibody detecting Periostin in IHC-P, IHC-Fr. Suitable for Human, Mouse, Rat.
- Biophysical QC for unrivalled batch-batch consistency
- Over 10 publications
대체 명칭 보기
OSF2, POSTN, Periostin, PN, Osteoblast-specific factor 2, OSF-2
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR20806] (AB215199)
Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling Periostin with ab215199 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive extracellular matrix staining on the stroma of human stomach (PMID : 15731169). Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR20806] (AB215199)
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling Periostin with ab215199 at 1/1000 dilution (0.516 μg/ml) followed by a Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880) at a Ready to use dilution. Positive staining on extracellular matrix of human breast carcinoma. The section was incubated with ab215199 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880) at Ready to use dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).
- IHC-Fr
Supplier Data
Immunohistochemistry (Frozen sections) - Anti-Periostin antibody [EPR20806] (AB215199)
Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized mouse stomach tissue labeling Periostin with ab215199 at 1/500 dilution ,followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive cytoplasmic staining in the gastric lamina propia mucosae, negative staining in the epithelial cells (PMID : 24418260).
The nuclear counter stain is DAPI (blue).
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
- IHC-P
Lab
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR20806] (AB215199)
Immunohistochemical analysis of paraffin-embedded (A) Stomach tissue from wild-type C57BL/6JGpt mice, and (B) Stomach tissue from Postn knockout mice labeling Periostin with ab215199 at 1/10000 dilution.
Positive staining on (A) Stomach tissue from wild-type C57BL/6JGpt mice and no staining on (B) Stomach tissue from Postn knockout mice.
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and Postn-KO homozygous mice (Strain ID : T011526).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR20806] (AB215199)
Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling Periostin with ab215199 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive extracellular matrix staining on the stroma of mouse colon (PMID : 15731169, PMID : 18443362). Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR20806] (AB215199)
Immunohistochemical analysis of paraffin-embedded rat stomach tissue labeling Periostin with ab215199 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive extracellular matrix staining on the stroma of rat stomach (PMID : 15731169). Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- IHC-Fr
Supplier Data
Immunohistochemistry (Frozen sections) - Anti-Periostin antibody [EPR20806] (AB215199)
Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized rat stomach tissue labeling Periostin with ab215199 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive cytoplasmic staining in the gastric lamina propia mucosae, negative staining in the epithelial cells (PMID : 24418260).
The nuclear counter stain is DAPI (blue).
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
- IHC-P
Lab
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR20806] (AB215199)
Immunohistochemical analysis of paraffin-embedded (A) Colon tissue from wild-type C57BL/6JGpt mice, and (B) Colon tissue from Postn knockout mice labeling Periostin with ab215199 at 1/10000 dilution.
Positive staining on (A) Colon tissue from wild-type C57BL/6JGpt mice and no staining on (B) Colon tissue from Postn knockout mice.
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and Postn-KO homozygous mice (Strain ID : T011526).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
- WB
Lab
Western blot - Anti-Periostin antibody [EPR20806] (AB215199)
Blocking and diluting buffer and concentration : 5% NFDM/TBST.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200,000 dilution.
The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and Postn-KO homozygous mice (Strain ID : T011526).
All lanes:
Western blot - Anti-Periostin antibody [EPR20806] (ab215199) at 1/1000 dilution
Lane 1:
Wild-type mouse stomach tissue lysate (female case1) at 20 µg
Lane 2:
Wild-type mouse stomach tissue lysate (female case2) at 20 µg
Lane 3:
Postn knockout mouse stomach tissue lysate (female case1) at 20 µg
Lane 4:
Postn knockout mouse stomach tissue lysate (female case2) at 20 µg
Lane 5:
Wild-type mouse colon tissue lysate (female case1) at 20 µg
Lane 6:
Wild-type mouse colon tissue lysate (female case2) at 20 µg
Lane 7:
Postn knockout mouse colon tissue lysate (female case1) at 20 µg
Lane 8:
Postn knockout mouse colon tissue lysate (female case2 at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Observed band size: 75-95 kDa,36 kDa
false
Exposure time: 180s
관련 conjugated 항체와 다양한 조성의 항체 (3)
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Anti-Periostin antibody [EPR20806] - BSA and Azide free
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519 Alexa Fluor® 488
Alexa Fluor® 488 Anti-Periostin antibody [EPR20806]
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565 Alexa Fluor® 555
Alexa Fluor® 555 Anti-Periostin antibody [EPR20806]
Reactivity 정보
제품 세부 정보
What is this antibody validated in?
Anti-Periostin antibody [EPR20806] (ab215199) is a rabbit recombinant monoclonal antibody and is validated for use in Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr) in Human, Mouse, Rat samples.
Trusted by the scientific community
Anti-Periostin [EPR20806] (ab215199) was first used in a scientific publication in 2017 and has been cited over 10 times in peer-reviewed journals.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.
Other related products
We have a range of other formats of antibody clone [EPR20806] also available for your convenience: ab215199, Carrier free - ab227049, Alexa Fluor® 555 - ab307126
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
특성 및 보관 정보
제형
Purification 테크닉
보관 버퍼
배송 시 보관 조건
적절한 단기 보관 기간
적절한 단기 보관 조건
적절한 장기 보관 조건
분주 정보
보관 정보
추가 정보
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The periostin protein contributes to the maintenance of tissue integrity and mechanical properties. It does not solely act; it forms part of a multiprotein complex with collagen and other extracellular matrix components. This involvement is necessary for maintaining cellular interactions and promoting tissue healing processes. Additionally periostin influences cell proliferation and migration impacting developmental and wound healing biology.
Pathways
Periostin is involved in the integrin signaling pathway and the Wnt/Β-catenin pathway both important for cell communication and movement. Through these pathways periostin interacts with integrins αvΒ3 and αvΒ5 facilitating cell adhesion and migration. The Wnt/Β-catenin pathway interaction involves several proteins including Frizzled receptors ensuring regulation of cellular proliferation and differentiation. These pathways make periostin a coordinator of complex cellular processes.
제품 프로토콜
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타겟 정보
제품이 사용된 논문 (25)
Recent publications for all applications. Explore the 전체 목록 and refine your search
TH open : companion journal to thrombosis and haemostasis 9:a27014242 PubMed41059381
2025
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Cell communication and signaling : CCS 23:411 PubMed41039578
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Cell communication and signaling : CCS 23:371 PubMed40796869
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Nature communications 16:3430 PubMed40210617
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Science advances 11:eadr7208 PubMed40203118
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Cancer communications (London, England) 45:172-177 PubMed39654335
2024
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Journal for immunotherapy of cancer 12: PubMed39067872
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Molecular cancer 23:120 PubMed38831402
2024
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Translational pediatrics 13:596-609 PubMed38715675
2024
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Nature communications 15:363 PubMed38191471
2024
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com