AB227049

Anti-Periostin antibody [EPR20806] - BSA and Azide free

Name: Anti-Periostin antibody [EPR20806] - BSA and Azide free
Brand: Abcam Limited
SKU: AB227049
Rating: 5 (1 reviews)

RabMAb
Recombinant
KO Validated
각 태그의 뜻

(1 리뷰)

(6 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal Periostin antibody. Carrier free. Suitable for IHC-Fr, IHC-P, WB and reacts with Mouse, Rat, Human samples. Cited in 6 publications.

9 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR20806] - BSA and Azide free (AB227049)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR20806] - BSA and Azide free (AB227049)

Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling Periostin with ab215199 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive extracellular matrix staining on the stroma of human stomach (PMID : 15731169). Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab215199).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR20806] - BSA and Azide free (AB227049)

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling Periostin with ab215199 at 1/1000 dilution (0.516 μg/ml) followed by a Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880) at a Ready to use dilution. Positive staining on extracellular matrix of human breast carcinoma. The section was incubated with ab215199 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880) at Ready to use dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab215199).

Immunohistochemistry (Frozen sections) - Anti-Periostin antibody [EPR20806] - BSA and Azide free (AB227049)

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Periostin antibody [EPR20806] - BSA and Azide free (AB227049)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized mouse stomach tissue labeling Periostin with ab215199 at 1/500 dilution ,followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive cytoplasmic staining in the gastric lamina propia mucosae, negative staining in the epithelial cells (PMID : 24418260).

The nuclear counter stain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab215199).

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR20806] - BSA and Azide free (AB227049)

This data was developed using ab215199, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded (A) Stomach tissue from wild-type C57BL/6JGpt mice, and (B) Stomach tissue from Postn knockout mice labeling Periostin with ab215199 at 1/10000 dilution.

Positive staining on (A) Stomach tissue from wild-type C57BL/6JGpt mice and no staining on (B) Stomach tissue from Postn knockout mice.

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and Postn-KO homozygous mice (Strain ID : T011526).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR20806] - BSA and Azide free (AB227049)

Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling Periostin with ab215199 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive extracellular matrix staining on the stroma of mouse colon (PMID : 15731169, PMID : 18443362). Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab215199).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR20806] - BSA and Azide free (AB227049)

Immunohistochemical analysis of paraffin-embedded rat stomach tissue labeling Periostin with ab215199 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive extracellular matrix staining on the stroma of rat stomach (PMID : 15731169). Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab215199).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Periostin antibody [EPR20806] - BSA and Azide free (AB227049)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized rat stomach tissue labeling Periostin with ab215199 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive cytoplasmic staining in the gastric lamina propia mucosae, negative staining in the epithelial cells (PMID : 24418260).

The nuclear counter stain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab215199).

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR20806] - BSA and Azide free (AB227049)

This data was developed using ab215199, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded (A) Colon tissue from wild-type C57BL/6JGpt mice, and (B) Colon tissue from Postn knockout mice labeling Periostin with ab215199 at 1/10000 dilution.

Positive staining on (A) Colon tissue from wild-type C57BL/6JGpt mice and no staining on (B) Colon tissue from Postn knockout mice.

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and Postn-KO homozygous mice (Strain ID : T011526).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Lab

Western blot - Anti-Periostin antibody [EPR20806] - BSA and Azide free (AB227049)

This data was developed using ab215199, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200,000 dilution.

The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and Postn-KO homozygous mice (Strain ID : T011526).

All lanes:

Western blot - Anti-Periostin antibody [EPR20806] (<a href='/ko/products/primary-antibodies/periostin-antibody-epr20806-ab215199'>ab215199</a>) at 1/1000 dilution

Lane 1:

Wild-type mouse stomach tissue lysate (female case1) at 20 µg

Lane 2:

Wild-type mouse stomach tissue lysate (female case2) at 20 µg

Lane 3:

Postn knockout mouse stomach tissue lysate (female case1) at 20 µg

Lane 4:

Postn knockout mouse stomach tissue lysate (female case2) at 20 µg

Lane 5:

Wild-type mouse colon tissue lysate (female case1) at 20 µg

Lane 6:

Wild-type mouse colon tissue lysate (female case2) at 20 µg

Lane 7:

Postn knockout mouse colon tissue lysate (female case1) at 20 µg

Lane 8:

Postn knockout mouse colon tissue lysate (female case2 at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 75-95 kDa,36 kDa

false

Exposure time: 180s

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20806

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-Fr, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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제품 세부 정보

ab227049 is the carrier-free version of ab215199.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

Do Not Freeze

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Periostin also known as POSTN or OSF-2 is an extracellular matrix protein with a molecular weight of approximately 90 kDa. It plays a mechanical role in tissue remodeling by binding to integrins which supports tissue repair and regeneration. Periostin is mainly expressed in collagen-rich tissues such as bone periodontal ligament and cardiac tissues. It is synthesized by osteoblasts fibroblasts and other stromal cells functioning as a structural protein that supports cell adhesion and migration.

Biological function summary

The periostin protein contributes to the maintenance of tissue integrity and mechanical properties. It does not solely act; it forms part of a multiprotein complex with collagen and other extracellular matrix components. This involvement is necessary for maintaining cellular interactions and promoting tissue healing processes. Additionally periostin influences cell proliferation and migration impacting developmental and wound healing biology.

Pathways

Periostin is involved in the integrin signaling pathway and the Wnt/β-catenin pathway both important for cell communication and movement. Through these pathways periostin interacts with integrins αvβ3 and αvβ5 facilitating cell adhesion and migration. The Wnt/β-catenin pathway interaction involves several proteins including Frizzled receptors ensuring regulation of cellular proliferation and differentiation. These pathways make periostin a coordinator of complex cellular processes.

The periostin protein is associated with conditions such as fibrotic diseases and cardiovascular disorders. In fibrosis periostin promotes collagen cross-linking exacerbating tissue stiffness. This involves increased interaction with proteins like collagen types I and V contributing to pathological fibrotic progression. In cardiovascular disorders elevated periostin levels after myocardial injury are linked with fibrotic scarring where it collaborates with matrix metalloproteinases to remodel the cardiac tissue architecture. Understanding these connections can lead to novel therapeutic strategies.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Induces cell attachment and spreading and plays a role in cell adhesion (PubMed : 12235007). Enhances incorporation of BMP1 in the fibronectin matrix of connective tissues, and subsequent proteolytic activation of lysyl oxidase LOX (By similarity).

See full target information POSTN

대체 명칭 보기

OSF2, POSTN, Periostin, PN, Osteoblast-specific factor 2, OSF-2

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제품이 사용된 논문 (6)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nature 632:411-418 PubMed39048831

2024

In vivo interaction screening reveals liver-derived constraints to metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Costanza Borrelli,Morgan Roberts,Davide Eletto,Marie-Didiée Hussherr,Hassan Fazilaty,Tomas Valenta,Atefeh Lafzi,Jonas A Kretz,Elena Guido Vinzoni,Andromachi Karakatsani,Srivathsan Adivarahan,Ardian Mannhart,Shoichiro Kimura,Ab Meijs,Farah Baccouche Mhamedi,Ilhan E Acar,Kristina Handler,Xenia Ficht,Randall J Platt,Salvatore Piscuoglio,Andreas E Moor

The Journal of clinical investigation 133: PubMed37824211

2023

Improving radiotherapy in immunosuppressive microenvironments by targeting complement receptor C5aR1.

Applications

Unspecified application

Species

Unspecified reactive species

Callum Beach,David MacLean,Dominika Majorova,Stavros Melemenidis,Dhanya K Nambiar,Ryan K Kim,Gabriel N Valbuena,Silvia Guglietta,Carsten Krieg,Mahnaz Darvish-Damavandi,Tatsuya Suwa,Alistair Easton,Lily Vs Hillson,Ashley K McCulloch,Ross K McMahon,Kathryn Pennel,Joanne Edwards,Sean M O'Cathail,Campbell S Roxburgh,Enric Domingo,Eui Jung Moon,Dadi Jiang,Yanyan Jiang,Qingyang Zhang,Albert C Koong,Trent M Woodruff,Edward E Graves,Tim Maughan,Simon Ja Buczacki,Manuel Stucki,Quynh-Thu Le,Simon J Leedham,Amato J Giaccia,Monica M Olcina

Nature 611:603-613 PubMed36352230

2022

Metastatic recurrence in colorectal cancer arises from residual EMP1 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Adrià Cañellas-Socias,Carme Cortina,Xavier Hernando-Momblona,Sergio Palomo-Ponce,Eoghan J Mulholland,Gemma Turon,Lidia Mateo,Sefora Conti,Olga Roman,Marta Sevillano,Felipe Slebe,Diana Stork,Adrià Caballé-Mestres,Antonio Berenguer-Llergo,Adrián Álvarez-Varela,Nicola Fenderico,Laura Novellasdemunt,Laura Jiménez-Gracia,Tamara Sipka,Lidia Bardia,Patricia Lorden,Julien Colombelli,Holger Heyn,Xavier Trepat,Sabine Tejpar,Elena Sancho,Daniele V F Tauriello,Simon Leedham,Camille Stephan-Otto Attolini,Eduard Batlle

Nature communications 13:5512 PubMed36127374

2022

Small soluble α-synuclein aggregates are the toxic species in Parkinson's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Derya Emin,Yu P Zhang,Evgeniia Lobanova,Alyssa Miller,Xuecong Li,Zengjie Xia,Helen Dakin,Dimitrios I Sideris,Jeff Y L Lam,Rohan T Ranasinghe,Antonina Kouli,Yanyan Zhao,Suman De,Tuomas P J Knowles,Michele Vendruscolo,Francesco S Ruggeri,Franklin I Aigbirhio,Caroline H Williams-Gray,David Klenerman

Cell stem cell 29:1213-1228.e8 PubMed35931031

2022

Dynamic and adaptive cancer stem cell population admixture in colorectal neoplasia.

Applications

Unspecified application

Species

Unspecified reactive species

Ester Gil Vasquez,Nadia Nasreddin,Gabriel N Valbuena,Eoghan J Mulholland,Hayley L Belnoue-Davis,Holly R Eggington,Ryan O Schenck,Valérie M Wouters,Pratyaksha Wirapati,Kathryn Gilroy,Tamsin R M Lannagan,Dustin J Flanagan,Arafath K Najumudeen,Sulochana Omwenga,Amy M B McCorry,Alistair Easton,Viktor H Koelzer,James E East,Dion Morton,Livio Trusolino,Timothy Maughan,Andrew D Campbell,Maurice B Loughrey,Philip D Dunne,Petros Tsantoulis,David J Huels,Sabine Tejpar,Owen J Sansom,Simon J Leedham

Scientific reports 10:13939 PubMed32883960

2020

Multi-omic single cell analysis resolves novel stromal cell populations in healthy and diseased human tendon.

Applications

Unspecified application

Species

Unspecified reactive species

Adrian R Kendal,Thomas Layton,Hussein Al-Mossawi,Louise Appleton,Stephanie Dakin,Rick Brown,Constantinos Loizou,Mark Rogers,Robert Sharp,Andrew Carr

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Periostin antibody [EPR20806] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR20806] - BSA and Azide free (AB227049)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR20806] - BSA and Azide free (AB227049)

Immunohistochemistry (Frozen sections) - Anti-Periostin antibody [EPR20806] - BSA and Azide free (AB227049)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR20806] - BSA and Azide free (AB227049)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR20806] - BSA and Azide free (AB227049)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR20806] - BSA and Azide free (AB227049)

Immunohistochemistry (Frozen sections) - Anti-Periostin antibody [EPR20806] - BSA and Azide free (AB227049)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR20806] - BSA and Azide free (AB227049)

Western blot - Anti-Periostin antibody [EPR20806] - BSA and Azide free (AB227049)

관련 conjugated 항체와 다양한 조성의 항체 (3)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Sample Prep & Detection Kits

Sample Prep & Detection Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (6)

Product promise