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AB4653

Anti-Peripherin antibody [8G2]

1

(1 리뷰)

|

(3 제품이 사용된 논문 )

Mouse Monoclonal Peripherin antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human, Cow samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Rat Prph.
2 이미지
Immunocytochemistry/ Immunofluorescence - Anti-Peripherin antibody [8G2] (AB4653)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Peripherin antibody [8G2] (AB4653)

Immunofluorescent analysis of SH-SY5Y (human bone marrow neuroblastoma cell) cells (A) and PC-12 (rat adrenal gland pheochromocytoma cell) cells (B), stained with ab4653 at 1/500 dilution (Red) and costained with chicken polyclonal anti-vimentin antibody at 1/10,000 dilution (Green). Blue : DAPI staining of nuclear DNA.

Western blot - Anti-Peripherin antibody [8G2] (AB4653)
  • WB

Supplier Data

Western blot - Anti-Peripherin antibody [8G2] (AB4653)

The band at ~57kDa corresponds to the peripherin protein. Bands at 50 and 25kDa detected in the mouse spinal cord lysate, correspond to the heavy and light chains of mouse IgG.

All lanes:

Western blot - Anti-Peripherin antibody [8G2] (ab4653) at 1/500 dilution

Lane 2:

Mouse spinal cord lysate

Lane 3:

Rat spinal cord lysate

Lane 4:

Cow spinal cord lysate

Lane 5:

SH-SY5Y (human bone marrow neuroblastoma cell) whole cell lysate

Lane 6:

PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate

Secondary

All lanes:

Anti-Mouse IgG

Predicted band size: 53 kDa

Observed band size: 25 kDa,50 kDa,57 kDa

false

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

8G2

Isotype

IgG1

Light chain 타입

kappa

Carrier free

No

Reacts with

Mouse, Rat, Cow, Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Rat Prph.

P21807

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대체품

Primary Antibodies

AB4666

Anti-Peripherin antibody

Immunocytochemistry/ Immunofluorescence - Anti-Peripherin antibody (AB4666)

  • 5
  • 19
Primary Antibodies

AB39374

Anti-Peripherin antibody

Immunocytochemistry/ Immunofluorescence - Anti-Peripherin antibody (AB39374)

  • 5
  • 7
Primary Antibodies

AB269861

Anti-Peripherin antibody [EPR23445-28] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-Peripherin antibody [EPR23445-28] - BSA and Azide free (AB269861)

  • 0
  • 0
Primary Antibodies

AB246502

Anti-Peripherin antibody [EPR23445-28]

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size

Flow Cytometry (Intracellular) - Anti-Peripherin antibody [EPR23445-28] (AB246502)

  • 5
  • 4

보완 제품

Primary Antibodies

AB245230

Anti-BRN3A antibody [EPR23257-285]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-BRN3A antibody [EPR23257-285] (AB245230)

  • 5
  • 2
Primary Antibodies

AB78078

Anti-beta III Tubulin antibody [2G10] - Neuronal Marker

BOND RX™ Validated|KO Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody [2G10] - Neuronal Marker (AB78078)

  • 5
  • 41
Primary Antibodies

AB183741

Anti-PHOX2B antibody [EPR14423] - C-terminal

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-PHOX2B antibody [EPR14423] - C-terminal (AB183741)

  • 5
  • 5
Primary Antibodies

AB81887

Anti-CGRP antibody [4901]

Immunocytochemistry/ Immunofluorescence - Anti-CGRP antibody [4901] (AB81887)

  • 5
  • 9
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>" }, "Cow": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
Purification 관련 사항
Purified antibody from TCS.
보관 버퍼
Preservative: 0.032% Sodium azide Constituents: PBS, 49.98% Glycerol (glycerin, glycerine)
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Peripherin also known as 8G2 or 7C5 is a protein expressed strongly in peripheral and certain central nervous system neurons. The mass of the peripherin protein is approximately 57 kDa. This intermediate filament protein structurally supports neurons aiding in their proper formation maintenance and regeneration. Peripherin localizes to the axons of mature neurons and serves important functions in the neuronal cytoskeleton and its stability.
Biological function summary

Peripherin impacts the nervous system's structure and dynamics. As part of the cytoskeleton it contributes to neurofilament networks which are essential for neuronal shape and mechanical integrity. Peripherin plays a role in neuronal differentiation and apoptosis. It operates independently but occasionally interacts with other cytoskeletal proteins like neurofilament light protein to perform its duties in neuronal cells.

Pathways

Peripherin is involved in processes associated with neuronal development and injury response. It features in neurogenesis pathways where it influences the growth and repair of axons. Peripherin interacts with proteins such as vimentin which helps manage cell dynamics and stress responses. The axonal transport pathway also utilizes peripherin to support axonal transport under stressful conditions ensuring cellular integrity and function.

Peripherin connects strongly to neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS) and spinal muscular atrophy (SMA). Its overexpression or aggregation can create abnormal filamentous structures that contribute to motor neuron degeneration. In ALS peripherin may interact with TAR DNA-binding protein 43 (TDP-43) and superoxide dismutase 1 (SOD1) proteins known to be significant in the disease pathogenesis. Understanding peripherin’s role in these diseases aids in unraveling mechanisms underlying neuronal degeneration.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Class-III neuronal intermediate filament protein (PubMed : 2624740, PubMed : 3339087). My form an independent structural network without the involvement of other neurofilaments or may cooperate with the neuronal intermediate filament proteins NEFL, NEFH, NEFM and INA to form a filamentous network (By similarity). Assembly of the neuronal intermediate filaments may be regulated by RAB7A (By similarity). Plays a role in the development of unmyelinated sensory neurons (By similarity). May be involved in axon elongation and axon regeneration after injury (PubMed : 2585054, PubMed : 7530776). Inhibits neurite extension in type II spiral ganglion neurons in the cochlea (By similarity).
See full target information Prph

대체 명칭 보기

Prph1, Prph, Peripherin
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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

The Journal of biological chemistry 287:6002-13 PubMed22187436

2011

Up-regulation of Cavβ3 subunit in primary sensory neurons increases voltage-activated Ca2+ channel activity and nociceptive input in neuropathic pain.

Applications

IHC-FrFl

Species

Rat

Li Li,Xue-Hong Cao,Shao-Rui Chen,Hee-Dong Han,Gabriel Lopez-Berestein,Anil K Sood,Hui-Lin Pan

The Journal of comparative neurology 520:81-99 PubMed21674494

2011

A-kinase anchoring protein 150 expression in a specific subset of TRPV1- and CaV 1.2-positive nociceptive rat dorsal root ganglion neurons.

Applications

WB, ICC/IF

Species

Rat, Rat

Katherine E Brandao,Mark L Dell'Acqua,S Rock Levinson

Journal of neurocytology 27:69-84 PubMed9609398

1998

The intermediate filament protein peripherin is a marker for cerebellar climbing fibres.

Applications

Unspecified application

Species

Unspecified reactive species

L Errante,D Tang,M Gardon,G Sekerkova,E Mugnaini,G Shaw
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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