Anti-Pet1 antibody [EPR30322-523]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pet1 antibody [EPR30322-523] (AB324873)

Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a mouse FEV expression vector containing a his tag; (B) HEK-293T transfected with empty vector containing a his tag. tissue labeling Pet1 with ab324873 at 1/2000 (0.257 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a mouse FEV expression vector containing a his tag, and no staining in (B) HEK-293T transfected with empty vector containing a his tag.
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pet1 antibody [EPR30322-523] (AB324873)

Immunohistochemical analysis of paraffin-embedded (A) SK-N-BE (Human human neuroblastoma neuroblast) cell pellets, (B) T-47D (human ductal breast epithelial tumor epithelial cell) cell pellets tissue labeling Pet1 with ab324873 at 1/100 (5.13 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in (A) SK-N-BE (Human human neuroblastoma neuroblast) cell pellets, no signal in （B) T-47D (human ductal breast epithelial tumor epithelial cell) cell pellets.
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pet1 antibody [EPR30322-523] (AB324873)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Pet1 with ab324873 at 1/100 (5.13 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining in mouse cerebrum.
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pet1 antibody [EPR30322-523] (AB324873)

Immunohistochemical analysis of paraffin-embedded Mouse E14.5 tissue labeling Pet1 with ab324873 at 1/100 (5.13 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in hindbrain of mouse E14.5.
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• WB

Supplier Data

Western blot - Anti-Pet1 antibody [EPR30322-523] (AB324873)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : T-47D

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Pet1 antibody [EPR30322-523] (ab324873) at 1/1000 dilution

Lane 1:

SK-N-BE(2) (human neuroblastoma neuroblast) whole cell lysate at 72 µg

Lane 2:

T-47D (human ductal breast epithelial tumor epithelial cell) whole cell lysate at 72 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 24 kDa,36 kDa

false

Exposure time: 180s