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AB232421

Anti-PEX19 antibody [EPR9266(B)] - BSA and Azide free

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Rabbit Recombinant Monoclonal PEX19 antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Rat samples.

대체 명칭 보기

HK33, PXF, OK/SW-cl.22, PEX19, Peroxisomal biogenesis factor 19, 33 kDa housekeeping protein, Peroxin-19, Peroxisomal farnesylated protein

3 이미지
Immunocytochemistry/ Immunofluorescence - Anti-PEX19 antibody [EPR9266(B)] - BSA and Azide free (AB232421)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-PEX19 antibody [EPR9266(B)] - BSA and Azide free (AB232421)

Immunocytochemistry/ Immunofluorescence analysis of MOLT-4 (Human lymphoblastic leukemia T lymphoblast) cells labeling PEX19 with Purified ab137072 at 1 : 100 (10.2 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1 : 200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137072).

Flow Cytometry (Intracellular) - Anti-PEX19 antibody [EPR9266(B)] - BSA and Azide free (AB232421)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-PEX19 antibody [EPR9266(B)] - BSA and Azide free (AB232421)

Intracellular flow cytometric analysis of permeabilized Jurkat (Human T cell leukemia cell line from peripheral blood) cells labeling PEX19 with ab137072 (red) or a rabbit IgG (negative) (green).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137072).

Immunoprecipitation - Anti-PEX19 antibody [EPR9266(B)] - BSA and Azide free (AB232421)
  • IP

Lab

Immunoprecipitation - Anti-PEX19 antibody [EPR9266(B)] - BSA and Azide free (AB232421)

ab137072 (purified) at 1 : 50 dilution (2μg) immunoprecipitating PEX19 in MOLT-4 whole cell lysate.
Lane 1 (input) : MOLT-4 (Human lymphoblastic leukemia T lymphoblast) whole cell lysate 10μg
Lane 2 (+) : ab137072 & MOLT-4 whole cell lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab137072 in MOLT-4 whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1 : 1000 dilution.
Blocking and diluting buffer : 5% NFDM/TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137072).

All lanes:

Immunoprecipitation - Anti-PEX19 antibody [EPR9266(B)] (<a href='/ko/products/primary-antibodies/pex19-antibody-epr9266b-ab137072'>ab137072</a>)

Predicted band size: 33 kDa

Observed band size: 33 kDa

false

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9266(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Rat, Human

Applications

WB, ICC/IF, Flow Cyt (Intra), IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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대체품

Primary Antibodies

AB95959

Anti-PEX19 antibody

Western blot - Anti-PEX19 antibody (AB95959)

  • 3
  • 1

보완 제품

Primary Antibodies

AB183734

Anti-GFP antibody [EPR14104]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] (AB183734)

  • 5
  • 24
Primary Antibodies

AB183885

Anti-PEX14 antibody

Immunocytochemistry/ Immunofluorescence - Anti-PEX14 antibody (AB183885)

  • 0
  • 0
Primary Antibodies

AB217059

Anti-PEX1 antibody

Immunoprecipitation - Anti-PEX1 antibody (AB217059)

  • 0
  • 0
Primary Antibodies

AB3421

Anti-PMP70 antibody

Western blot - Anti-PMP70 antibody (AB3421)

  • 4
  • 3
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p><a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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제품 세부 정보

ab232421 is the carrier-free version of ab137072.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
Do Not Freeze
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

PEX19 also known as peroxisomal biogenesis factor 19 is a protein important for the assembly of peroxisomal membranes. It has a molecular mass of approximately 36 kDa and is expressed across multiple tissues in the human body with particularly high levels in the liver and kidneys. This protein functions as a chaperone and is essential for the import of peroxisomal membrane proteins. It plays a significant role in the binding of these peroxisomal proteins and helps in their transportation to the peroxisomes.
Biological function summary

The protein plays a central role in peroxisome biogenesis facilitating the correct assembly and maintenance of the peroxisome. PEX19 interacts with other components in the peroxisomal import machinery such as PEX3 and PEX16 forming a complex responsible for the recognition and stabilization of peroxisomal membrane proteins. Through these interactions it ensures the functional integrity of peroxisomes which are important for metabolic tasks like fatty acid beta-oxidation and detoxification of hydrogen peroxide.

Pathways

PEX19 is involved in the peroxisomal protein import pathway and the broader lipid metabolism pathway. It plays an important role in peroxisomal membrane assembly through its interactions with PEX3 a receptor on the peroxisomal membrane that facilitates protein docking. This process is essential for maintaining efficient peroxisomal functions as disruptions may lead to metabolic imbalances involving lipids and reactive oxygen species.

PEX19 has significant implications in peroxisomal biogenesis disorders including Zellweger spectrum disorders. Mutations or deficiencies in PEX19 can lead to impaired peroxisome formation manifesting in severe metabolic dysfunctions. PEX19 is linked to disorders characterized by neurological deficits liver dysfunction and skeletal abnormalities. Its relationship with PEX3 is also critical in these disorders as any disruptions in their interaction can exacerbate the condition's severity.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Necessary for early peroxisomal biogenesis. Acts both as a cytosolic chaperone and as an import receptor for peroxisomal membrane proteins (PMPs). Binds and stabilizes newly synthesized PMPs in the cytoplasm by interacting with their hydrophobic membrane-spanning domains, and targets them to the peroxisome membrane by binding to the integral membrane protein PEX3. Excludes CDKN2A from the nucleus and prevents its interaction with MDM2, which results in active degradation of TP53.
See full target information PEX19
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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