AB70175

Anti-PFKFB2 antibody

Name: Anti-PFKFB2 antibody
Brand: Abcam Limited
SKU: AB70175
Rating: 5 (3 reviews)

(3 리뷰들)

(2 제품이 사용된 논문 )

Rabbit Polyclonal PFKFB2 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human PFKFB2.

2 이미지

Unknown

Western blot - Anti-PFKFB2 antibody (AB70175)

Various other bands are detected, from 26 to over 117kDa.

All lanes:

Western blot - Anti-PFKFB2 antibody (ab70175) at 1/500 dilution

Lane 1:

Extracts from Jurkat cells at 30 µg

Lane 2:

Extracts from Jurkat cells at 30 µg with immunising peptide

Predicted band size: 58 kDa

Observed band size: 58 kDa

false

AbReview21725****

Western blot - Anti-PFKFB2 antibody (AB70175)

Blocking Step : 5% BSA for 1 hour at 22°C

All lanes:

Western blot - Anti-PFKFB2 antibody (ab70175) at 1/500 dilution

All lanes:

Whole cell lysate of mouse NIH 3T£ cells at 25 µg

Secondary

All lanes:

An HRP-conjugated Sheep anti-rabbit monoclonal at 1/5000 dilution

Predicted band size: 58 kDa

false

Exposure time: 8min

This image is courtesy of an anonymous Abreview

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human PFKFB2. The exact immunogen used to generate this antibody is proprietary information.

O60825

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

보관 버퍼

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

보관 정보

Stable for 12 months at -20°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Phosphofructokinase/fructose-26-bisphosphatase 2 often called PFKFB2 plays a significant role in the regulation of glycolysis and gluconeogenesis. This bifunctional enzyme has a molecular mass of approximately 55 kDa. PFKFB2 catalyzes the conversion between fructose-6-phosphate and fructose-26-bisphosphate. It is mainly expressed in cardiac tissue though expression has been noticed in other tissues such as skeletal muscle and liver.

Biological function summary

PFKFB2 regulates the levels of fructose-26-bisphosphate an important metabolic signal molecule. This molecule modulates the activity of key enzymes in glycolysis. PFKFB2 forms part of a complex that controls the glycolytic flux in response to hormonal and nutritional changes. By increasing levels of fructose-26-bisphosphate it strongly activates phosphofructokinase-1 (PFK-1) pushing cells towards glycolytic metabolism under conditions of increased energy demand.

Pathways

PFKFB2 directly influences the glycolysis and gluconeogenesis pathways. The regulation of these pathways by PFKFB2 ties it closely to energy metabolism. Additionally it has functional connections with enzymes such as phosphofructokinase-1 (PFK-1) and fructose-16-bisphosphatase (FBPase) helping to balance the glycolytic and gluconeogenic flux according to cellular needs.

Studies implicate PFKFB2 in conditions such as ischemic heart disease and certain cancers. Ischemic heart disease involves energy metabolism disruption where PFKFB2 plays a role in sustaining cardiac energy supply under oxygen-limited conditions. In cancer PFKFB2's altered expression correlates with the Warburg effect where cancer cells favor glycolysis even in the presence of oxygen. This connection makes PFKFB2 relevant to research fields exploring metabolic interventions in cancer therapeutics.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Synthesis and degradation of fructose 2,6-bisphosphate.

See full target information PFKFB2

대체 명칭 보기

PFK/FBPase 2, PFKFB2

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Biochemical and biophysical research communication 515:37-43 PubMed31122697

2019

miR-613 inhibits Warburg effect in gastric cancer by targeting PFKFB2.

Applications

Unspecified application

Species

Unspecified reactive species

Haipeng Liu,Kang Chen,Li Wang,Xiangting Zeng,Zeping Huang,Mei Li,Peng Dong,Xiao Chen

JCI insight 1: PubMed27398409

2016

Fibulin-1 regulates the pathogenesis of tissue remodeling in respiratory diseases.

Applications

Unspecified application

Species

Unspecified reactive species

Gang Liu,Marion A Cooley,Andrew G Jarnicki,Alan C-Y Hsu,Prema M Nair,Tatt Jhong Haw,Michael Fricker,Shaan L Gellatly,Richard Y Kim,Mark D Inman,Gavin Tjin,Peter A B Wark,Marjorie M Walker,Jay C Horvat,Brian G Oliver,W Scott Argraves,Darryl A Knight,Janette K Burgess,Philip M Hansbro

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PFKFB2 antibody

Western blot - Anti-PFKFB2 antibody (AB70175)

Western blot - Anti-PFKFB2 antibody (AB70175)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (2)

Product promise