JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB317160

Anti-PFKFB4 antibody [HL2204]

Be the first to review this product! 리뷰 제출

|

(0 출판물)

Rabbit Recombinant Monoclonal PFKFB4 antibody. Suitable for ICC/IF, WB and reacts with Human, Mouse, Rat, Transfected cell lysate samples. Immunogen corresponding to Recombinant Fragment Protein within Human PFKFB4.

대체 명칭 보기

PFK/FBPase 4, PFKFB4

2 이미지
Immunocytochemistry/ Immunofluorescence - Anti-PFKFB4 antibody [HL2204] (AB317160)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PFKFB4 antibody [HL2204] (AB317160)

Immunofluorescence analysis of U87-MG cells fixed in ice-cold MeOH for 5 min labelling PFKFB4 protein at cytoplasm and nucleus using ab317160 at a 1/500 dilution (Green).
alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody at 1/1000 dilution (Red).
DAPI was used for nuclear staining (Blue).

Western blot - Anti-PFKFB4 antibody [HL2204] (AB317160)
  • WB

Supplier Data

Western blot - Anti-PFKFB4 antibody [HL2204] (AB317160)

Whole cell extract (30 ug) was separated by 10% SDS-PAGE and the membrane was blotted with ab317160 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-PFKFB4 antibody [HL2204] (ab317160) at 1/1000 dilution

All lanes:

Rat2 whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL2204

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human PFKFB4.

Q16877

style
left-column

보완 제품

Primary Antibodies

AB115730

Anti-Glucose Transporter GLUT1 antibody [EPR3915]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] (AB115730)

  • 4
  • 20
Primary Antibodies

AB181861

Anti-PFKFB3 antibody [EPR12594]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-PFKFB3 antibody [EPR12594] (AB181861)

  • 5
  • 6
Primary Antibodies

AB209846

Anti-HKDC1 antibody [EPR19835]

RabMAb|Recombinant

Immunoprecipitation - Anti-HKDC1 antibody [EPR19835] (AB209846)

  • 0
  • 0
Primary Antibodies

AB76495

Anti-Thymidine Kinase 1/TK1 antibody [EPR3193]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-Thymidine Kinase 1/TK1 antibody [EPR3193] (AB76495)

  • 4
  • 2
style
left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Transfected cell lysate": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }
style
left-column

제품 세부 정보

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

특성 및 보관 정보

제형
Liquid
보관 버퍼
pH: 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
style
left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

PFKFB4 also known as 6-phosphofructo-2-kinase/fructose-26-bisphosphatase 4 is an enzyme with a molecular mass of approximately 66 kDa. It regulates the levels of fructose-26-bisphosphate by catalyzing both its synthesis and degradation. This enzyme predominantly exists in the cytoplasm and shows higher expression in tissues with fast cell growth and high glycolytic demand like the liver and various cancerous cells. PFKFB4 serves as an important regulatory node in cellular metabolism by controlling the flux of glucose through glycolysis.
Biological function summary

PFKFB4 influences metabolic reprogramming during cell proliferation and stress conditions. It is particularly important in the glycolytic shift known as the Warburg effect where cancer cells prefer glycolysis over oxidative phosphorylation leading to rapid glycolysis and lactic acid production even in the presence of oxygen. The enzyme is often part of a complex involving other glycolytic enzymes affecting both energy production and biosynthetic processes necessary for tumor growth and survival.

Pathways

PFKFB4 plays an integral role in the glycolytic and pentose phosphate pathways. It regulates glycolysis by modulating the concentration of fructose-26-bisphosphate which is an allosteric activator of phosphofructokinase-1 (PFK-1). PFKFB4 also interfaces with AMPK signaling which ties energy status of the cell to metabolic pathways further linking it to responses to energy stress and hypoxia in cancer cells.

PFKFB4 has strong associations with cancer and metabolic disorders. Overexpression of PFKFB4 is found in several cancer types including breast and prostate cancer where it supports anabolic processes and survival under metabolic stress. The enzyme closely relates to oncogenic proteins such as MYC which drives glycolytic gene expression and HIF-1α a factor that promotes glycolysis under hypoxia. Analyzing PFKFB4 provides insights into therapeutic approaches focused on targeting metabolic pathways in cancer.
style
left-column
style
left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

타겟 정보

Synthesis and degradation of fructose 2,6-bisphosphate.
See full target information PFKFB4
style
left-column
style
left-column
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com