AB241093

Anti-PFKL antibody

Be the first to review this product! 리뷰 제출

(3 제품이 사용된 논문 )

Rabbit Polyclonal PFKL antibody. Suitable for IP, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human PFKL aa 1-50.

대체 명칭 보기

ATP-PFK, PFK-L, 6-phosphofructokinase type B, Phosphofructo-1-kinase isozyme B, Phosphohexokinase, PFK-B, PFKL

2 이미지

Supplier Data

Immunoprecipitation - Anti-PFKL antibody (AB241093)

PFKL was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241093 at 6 μg/reaction. Western blot was performed from the immunoprecipitate using ab241093 at 1 μg/ml.

Lane 1 : ab241093 IP in HeLa whole cell lysate.
Lane 2 : Control IgG IP in HeLa whole cell lysate.

Detection : Chemiluminescence with exposure time of 3 minutes.

Lysates prepared in NETN lysis buffer.

All lanes:

Immunoprecipitation - Anti-PFKL antibody (ab241093)

Predicted band size: 85 kDa

false

Supplier Data

Western blot - Anti-PFKL antibody (AB241093)

Lysates prepared in NETN lysis buffer.

All lanes:

Western blot - Anti-PFKL antibody (ab241093) at 0.4 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Predicted band size: 85 kDa

true

Exposure time: 3min

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human PFKL aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P17858

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

style

left-column

특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

style

left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

PFKL also known as phosphofructokinase liver type is an important enzyme in glycolysis. It catalyzes the conversion of fructose-6-phosphate to fructose-16-bisphosphate. This enzyme plays a significant role in regulating the glycolytic flux due to its allosteric nature. Weighing approximately 85 kDa PFKL is found abundantly in liver tissues but you can also find it in various other tissues such as muscle and brain. Its expression adapts according to the metabolic demand reflecting its importance in energy homeostasis.

Biological function summary

Phosphofructokinase liver type is an integral component of cellular energy production. It functions in the cytoplasmic compartment and often operates as a part of a tetrameric complex. The enzyme activity is subject to regulation by several metabolites including ATP ADP and citrate which modulate its activity for efficient energy use. It acts as a metabolic checkpoint influencing the rate of glucose breakdown in response to cellular energy needs.

Pathways

Phosphofructokinase liver type plays a central role in the glycolytic pathway. This pathway is critical for ATP generation especially in conditions where cells rely heavily on glucose. It cooperates with other enzymes like hexokinase and pyruvate kinase to ensure a smooth conversion of glucose to pyruvate facilitating subsequent processes like the citric acid cycle. By integrating signals from multiple pathways it serves as a nodal point for metabolic control affecting various physiological and biochemical processes.

Dysregulation of phosphofructokinase liver type can lead to metabolic diseases such as type 2 diabetes and cancer. Altered activity or expression of PFKL influences glucose metabolism potentially resulting in insulin resistance or enhanced tumor glycolysis respectively. Moreover PFKL shares connections with other proteins like insulin receptor substrates in diabetes and oncogenes in cancer influencing disease progression and response to therapies. Understanding PFKL's function and regulation can aid in developing strategies for these conditions.

style

left-column

style

left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

타겟 정보

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis (PubMed : 22923583). Negatively regulates the phagocyte oxidative burst in response to bacterial infection by controlling cellular NADPH biosynthesis and NADPH oxidase-derived reactive oxygen species. Upon macrophage activation, drives the metabolic switch toward glycolysis, thus preventing glucose turnover that produces NADPH via pentose phosphate pathway (By similarity).

See full target information PFKL

style

left-column

제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

NAR cancer 7:zcaf024 PubMed40918646

2025

Loss of ribosomal protein uL14 enables tumor escape from T cell immunosurveillance.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Dopler,Edwin S Kyei-Baffour,Mandy Kerkhoff,Ferhat Alkan,Yuval Malka,Kelly Hoefakker,Rob van der Kammen,Liesbeth Hoekman,Onno Bleijerveld,Antonia Bradaric,Maarten Altelaar,Jonathan W Yewdell,Pia Kvistborg,William J Faller

Neoplasma 69:1041-1053 PubMed35723199

2022

Exosomes from M2 macrophages promoted glycolysis in FaDu cells by inhibiting PDLIM2 expression to stabilize PFKL.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Wang,Guang-Yao Li,Lei Zhou,Huai-Li Jiang,Yue Yang,Hai-Tao Wu

Cell death & disease 12:408 PubMed33859186

2021

The role of S-nitrosylation of PFKM in regulation of glycolysis in ovarian cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Wenwen Gao,Mengqiu Huang,Xi Chen,Jianping Chen,Zhiwei Zou,Linlin Li,Kaiyuan Ji,Zhirui Nie,Bingsheng Yang,Zibo Wei,Pengfei Xu,Junshuang Jia,Qianbing Zhang,Hongfen Shen,Qianli Wang,Keyi Li,Lingqun Zhu,Meng Wang,Shuangyan Ye,Sisi Zeng,Ying Lin,Zhili Rong,Yang Xu,Peng Zhu,Hui Zhang,Bingtao Hao,Qiuzhen Liu

제품이 사용된 논문 모두 보기

style

left-column

style

left-column

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PFKL antibody

Immunoprecipitation - Anti-PFKL antibody (AB241093)

Western blot - Anti-PFKL antibody (AB241093)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (3)

Product promise