AB233109

Anti-PFKP antibody

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(2 제품이 사용된 논문 )

Rabbit Polyclonal PFKP antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PFKP aa 550 to C-terminus.

대체 명칭 보기

PFKF, PFKP, ATP-PFK, PFK-P, 6-phosphofructokinase type C, Phosphofructo-1-kinase isozyme C, Phosphohexokinase, PFK-C

8 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKP antibody (AB233109)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKP antibody (AB233109)

Formalin-fixed, paraffin-embedded human prostate cancer tissue stained for PFKP using ab233109 at 10 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKP antibody (AB233109)

Formalin-fixed, paraffin-embedded human lung cancer tissue stained for PFKP using ab233109 at 10 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKP antibody (AB233109)

Formalin-fixed, paraffin-embedded human liver tissue stained for PFKP using ab233109 at 10 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKP antibody (AB233109)

Formalin-fixed, paraffin-embedded human brain tissue stained for PFKP using ab233109 at 10 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKP antibody (AB233109)

Formalin-fixed, paraffin-embedded human stomach tissue stained for PFKP using ab233109 at 10 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKP antibody (AB233109)

Formalin-fixed, paraffin-embedded human breast cancer tissue stained for PFKP using ab233109 at 10 μg/ml in immunohistochemical analysis. DAB staining.

Supplier Data

Western blot - Anti-PFKP antibody (AB233109)

All lanes:

Western blot - Anti-PFKP antibody (ab233109) at 5 µg/mL

All lanes:

Recombinant human PFKP protein

Predicted band size: 86 kDa

false

Supplier Data

Western blot - Anti-PFKP antibody (AB233109)

All lanes:

Western blot - Anti-PFKP antibody (ab233109) at 3 µg/mL

Lane 1:

A549 (human lung carcinoma cell line) cell lysate

Lane 2:

Pig heart lysate

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate

Lane 4:

Pig skeletal muscle lysate

Secondary

All lanes:

HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution

Predicted band size: 86 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human PFKP aa 550 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q01813

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-5 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

Antigen-specific affinity chromatography followed by Protein A affinity chromatography.

보관 버퍼

pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

PFKP also known as 6-phosphofructokinase platelet type functions as an important glycolytic enzyme that catalyzes the phosphorylation of fructose 6-phosphate to fructose 16-bisphosphate using ATP. This process shows the rate-limiting step in glycolysis. The PFKP protein weighs approximately 85 kDa. Expression occurs in various tissues with high presence in platelets and muscle tissues indicating its significant role in energy metabolism.

Biological function summary

PFKP plays an essential role in maintaining energy supply ensuring efficient glycolysis during high energy demands. It associates with other isoforms PFKM (muscle) and PFKL (liver) to form a tetrameric complex which can adapt its activity based on the energy needs of the cell. This adaptability defines its contribution to cellular metabolism regulation considering energy substrates and signaling molecules.

Pathways

The function of PFKP ties closely to glycolysis and the larger metabolic pathways managing energy production such as the tricarboxylic acid (TCA) cycle. Within these pathways PFKP interacts with other metabolic regulators including AMP-activated protein kinase (AMPK) which influences cell metabolism in response to energy availability. Connections with PFKL and PFKM reveal that PFKP's activity relates to the overall glycolytic flux and energy balance.

PFKP's alterations contribute to metabolic conditions like cancer and diabetes. Its dysregulation in cancer cells supports the Warburg effect where tumor cells prefer glycolysis. The link with metabolic proteins such as AMPK also indicates a potential role in diabetes as both disorders involve shifts in energy metabolism. Understanding PFKP's impact on these diseases helps in developing therapeutic strategies targeting metabolic pathways.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.

See full target information PFKP

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Translational psychiatry 15:361 PubMed41053001

2025

Restoration of NEXMIF expression rescues abnormalities in gene transcription, neuron maturation and autistic-like behaviors in Nexmif knockout mice.

Applications

Unspecified application

Species

Unspecified reactive species

KathrynAnn Odamah,Heng-Ye Man

The Journal of cell biology 221: PubMed35670764

2022

Fbxo7 promotes Cdk6 activity to inhibit PFKP and glycolysis in T cells.

Applications

Unspecified application

Species

Unspecified reactive species

Rebecca Harris,Ming Yang,Christina Schmidt,Chloe Royet,Sarbjit Singh,Amarnath Natarajan,May Morris,Christian Frezza,Heike Laman

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PFKP antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKP antibody (AB233109)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKP antibody (AB233109)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKP antibody (AB233109)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKP antibody (AB233109)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKP antibody (AB233109)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKP antibody (AB233109)

Western blot - Anti-PFKP antibody (AB233109)

Western blot - Anti-PFKP antibody (AB233109)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (2)

Product promise