AB244218

Anti-PGAM5 antibody [CL0624]

KO Validated
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(4 제품이 사용된 논문 )

Mouse Monoclonal PGAM5 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PGAM5 aa 50 to C-terminus.

대체 명칭 보기

Bcl-XL-binding protein v68, Phosphoglycerate mutase family member 5, PGAM5

8 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM5 antibody [CL0624] (AB244218)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM5 antibody [CL0624] (AB244218)

Paraffin-embedded human small intestine tissue stained for PGAM5 using ab244218 at 1/200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM5 antibody [CL0624] (AB244218)

Paraffin-embedded human uterus tissue stained for PGAM5 using ab244218 at 1/200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM5 antibody [CL0624] (AB244218)

Paraffin-embedded human kidney tissue stained for PGAM5 using ab244218 at 1/200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM5 antibody [CL0624] (AB244218)

Paraffin-embedded human liver tissue stained for PGAM5 using ab244218 at 1/200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM5 antibody [CL0624] (AB244218)

Paraffin-embedded human testis tissue stained for PGAM5 using ab244218 at 1/200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM5 antibody [CL0624] (AB244218)

Paraffin-embedded human tonsil tissue stained for PGAM5 using ab244218 at 1/200 dilution in immunohistochemical analysis.

Lab

Western blot - Anti-PGAM5 antibody [CL0624] (AB244218)

ab244218 Anti-PGAM5 antibody [CL0624] was shown to specifically react with PGAM5 in HeLa wild-type cells (ab255928). Loss of signal was observed when knockout cell line ab265141 (knockout cell lysate ab257581) was used. Wild-type and PGAM5 knockout samples were subjected to SDS-PAGE. ab244218 and Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (ab52866) were incubated overnight at 4°C at 1 μg/ml and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-PGAM5 antibody [CL0624] (ab244218) at 1 µg/mL

Lane 1:

Wild-type HeLa lysate at 20 µg

Lane 2:

PGAM5 knockout HeLa lysate at 20 µg

Lane 2:

Western blot - Human PGAM5 knockout HeLa cell line (<a href='/ko/products/cell-lines/human-pgam5-knockout-hela-cell-line-ab265141'>ab265141</a>)

Lane 3:

HepG2 lysate at 20 µg

Lane 4:

Daudi lysate at 20 µg

Predicted band size: 32 kDa

Observed band size: 32 kDa

false

Supplier Data

Western blot - Anti-PGAM5 antibody [CL0624] (AB244218)

All lanes:

Western blot - Anti-PGAM5 antibody [CL0624] (ab244218) at 1 µg

All lanes:

RT4 (human urinary bladder cancer cell line) cell lysate

Predicted band size: 32 kDa

false

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

CL0624

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human PGAM5 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q96HS1

Epitope

Binds to an epitope located within the peptidesequence NWDRREPLSLINVRK as determined by overlapping synthetic peptides.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

PGAM5 also known as Phosphoglycerate Mutase Family Member 5 is a mitochondrial protein with a known mass of around 32 kDa. It acts as a serine/threonine phosphatase without intrinsic catalytic phosphoglycerate mutase activity. PGAM5 localizes to the inner membrane of mitochondria and exhibits expression in tissues where metabolic regulation is significant. It plays a role in removing phosphate groups from target substrates influencing multiple cellular mechanisms.

Biological function summary

PGAM5 impacts pathways related to cellular stress responses and energy metabolism. It participates in programmed cell death and modulates mitochondrial dynamics. It can serve as part of a complex with other proteins adjusting the balance between mitophagy and apoptosis. Through interaction with key signaling molecules PGAM5 bridges various biochemical processes relevant to cell survival and energy utilization.

Pathways

Its function integrates into mitophagy and necroptosis. PGAM5 collaborates with proteins like PINK1 and PARKIN contributing to the clearance of damaged mitochondria via the mitophagy pathway. In necroptosis PGAM5 interacts with RIPK1 and RIPK3 regulating necrotic cell death. These interactions position PGAM5 at the crossroads of pathways critical to maintaining cellular homeostasis and response to stress.

PGAM5 relates to neurodegenerative diseases and cancer. Mutations or dysregulation of PGAM5 connect to Parkinson's disease where impaired mitochondrial function and mitophagy play a role. In cancer altered PGAM5 activity may disrupt normal apoptotic processes leading to unchecked cell growth. In these contexts PGAM5's interaction with proteins like PINK1 PARKIN RIPK1 and RIPK3 underpins its relevance to disease mechanisms.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Mitochondrial serine/threonine phosphatase that dephosphorylates various substrates and thus plays a role in different biological processes including cellular senescence or mitophagy (PubMed : 24746696, PubMed : 32439975). Modulates cellular senescence by regulating mitochondrial dynamics. Mechanistically, participates in mitochondrial fission through dephosphorylating DNM1L/DRP1 (PubMed : 32439975). Additionally, dephosphorylates MFN2 in a stress-sensitive manner and consequently protects it from ubiquitination and degradation to promote mitochondrial network formation (PubMed : 37498743). Regulates mitophagy independent of PARKIN by interacting with and dephosphorylating FUNDC1, which interacts with LC3 (PubMed : 24746696). Regulates anti-oxidative response by forming a tertiary complex with KEAP1 and NRF2 (PubMed : 18387606). Regulates necroptosis by acting as a RIPK3 target and recruiting the RIPK1-RIPK3-MLKL necrosis 'attack' complex to mitochondria (PubMed : 22265414).

See full target information PGAM5

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제품이 사용된 논문 (4)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Medicine 102:e35452 PubMed37800782

2023

NIFK as a potential prognostic biomarker in colorectal cancer correlating with immune infiltrates.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Tan,Yuqi He,Linhui Wu,Shiquan Liu,Peng Peng,Jiean Huang

Experimental and therapeutic medicine 23:359 PubMed35493422

2022

Moxibustion alleviates chronic heart failure by regulating mitochondrial dynamics and inhibiting autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Ran Xia,Wei Wang,Bing Gao,Qiang Ma,Jing Wang,Xiaohua Dai,Qingling Li

Redox biology 52:102301 PubMed35358851

2022

MnTE-2-PyP protects fibroblast mitochondria from hyperglycemia and radiation exposure.

Applications

Unspecified application

Species

Unspecified reactive species

Arpita Chatterjee,Isin T Sakallioglu,Divya Murthy,Elizabeth A Kosmacek,Pankaj K Singh,J Tyson McDonald,Robert Powers,Rebecca E Oberley-Deegan

International journal of molecular sciences 22: PubMed34360749

2021

Inhibition of Cardiac RIP3 Mitigates Early Reperfusion Injury and Calcium-Induced Mitochondrial Swelling without Altering Necroptotic Signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Csaba Horvath,Megan Young,Izabela Jarabicova,Lucia Kindernay,Kristina Ferenczyova,Tanya Ravingerova,Martin Lewis,M Saadeh Suleiman,Adriana Adameova

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PGAM5 antibody [CL0624]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM5 antibody [CL0624] (AB244218)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM5 antibody [CL0624] (AB244218)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM5 antibody [CL0624] (AB244218)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM5 antibody [CL0624] (AB244218)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM5 antibody [CL0624] (AB244218)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM5 antibody [CL0624] (AB244218)

Western blot - Anti-PGAM5 antibody [CL0624] (AB244218)

Western blot - Anti-PGAM5 antibody [CL0624] (AB244218)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Epitope

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Assay Kits

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (4)

Product promise