AB217966

Anti-PGE2 receptor EP4 subtype antibody

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(5 제품이 사용된 논문 )

Rabbit Polyclonal PGE2 receptor EP4 subtype antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human PTGER4 aa 150-200 conjugated to Keyhole Limpet Haemocyanin.

2 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGE2 receptor EP4 subtype antibody (AB217966)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGE2 receptor EP4 subtype antibody (AB217966)

Immunohistochemical analysis of formalin-fixed paraffin-embedded sections of human lung carcinoma tissue labeling PGE2 receptor EP4 with ab217966 at a 1/200 dilution, followed by conjugation to a secondary antibody and DAB staining.

Lab

Western blot - Anti-PGE2 receptor EP4 subtype antibody (AB217966)

All lanes:

Western blot - Anti-PGE2 receptor EP4 subtype antibody (ab217966) at 1/300 dilution

All lanes:

Raji cell lysates

Secondary

All lanes:

Conjugated secondary antibody at 1/20000 dilution

Predicted band size: 53 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human PTGER4 aa 150-200 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P35408

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/300", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The PGE2 receptor EP4 subtype also known as PTGER4 is a member of the G protein-coupled receptor (GPCR) family. This receptor binds prostaglandin E2 and plays a role in various physiological processes. The protein has an approximate mass of 65 kDa. EP4 receptor is widely expressed in tissues such as the immune system bone gastrointestinal tract and reproductive organs indicating its diverse functional implications. Additionally EP4 receptor is activated by PGE2 EP4 agonists which help in modulating its activity.

Biological function summary

EP4 receptor influences numerous functions including inflammation immune response and bone metabolism. The receptor is not part of a larger complex but interacts with different signaling molecules and secondary messengers during its activity. It modulates responses mainly through cAMP which further amplifies or reduces cellular responses depending on the context. These biological roles display the versatile effects EP4 can exert during various physiological scenarios.

Pathways

The EP4 receptor is a significant component in the prostaglandin signaling pathway. This pathway involves the conversion of arachidonic acid to prostaglandin E2 followed by binding to EP4 triggering downstream effects via cAMP-related signals. Within this pathway it interacts closely with proteins like adenylate cyclase which plays a role in regulating cAMP levels. EP4 also participates in the Wnt signaling pathway where it can either enhance or inhibit processes by interacting with other signaling cascade proteins.

EP4 receptor has connections with conditions like rheumatoid arthritis and bone-related diseases. In rheumatoid arthritis excessive signaling through EP4 exacerbates inflammatory responses in joint tissue. The receptor potentially associates with inflammatory cytokines like tumor necrosis factor-alpha (TNF-alpha) contributing to disease severity. In bone-related diseases such as osteoporosis EP4 influences bone remodeling and homeostasis linking to proteins involved in bone formation and resorption dynamics.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. Has a relaxing effect on smooth muscle. May play an important role in regulating renal hemodynamics, intestinal epithelial transport, adrenal aldosterone secretion, and uterine function.

See full target information PTGER4

대체 명칭 보기

PTGER2, PTGER4, Prostaglandin E2 receptor EP4 subtype, PGE receptor EP4 subtype, PGE2 receptor EP4 subtype, Prostanoid EP4 receptor

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제품이 사용된 논문 (5)

Recent publications for all applications. Explore the 전체 목록and refine your search

eLife 13: PubMed40028769

2025

The Kv2.2 channel mediates the inhibition of prostaglandin E2 on glucose-stimulated insulin secretion in pancreatic β-cells.

Applications

Unspecified application

Species

Unspecified reactive species

Chengfang Pan,Ying Liu,Liangya Wang,Wen-Yong Fan,Yunzhi Ni,Xuefeng Zhang,Di Wu,Chenyang Li,Jin Li,Zhaoyang Li,Rui Liu,Changlong Hu

Cellular and molecular life sciences : CMLS 79:303 PubMed35588334

2022

Macrophages evoke autophagy of hepatic stellate cells to promote liver fibrosis in NAFLD mice via the PGE2/EP4 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Cao,Weili Mai,Rui Li,Shuwei Deng,Lan Li,Yanxi Zhou,Qiushi Qin,Yue Zhang,Xingang Zhou,Ming Han,Pu Liang,Yonghong Yan,Yu Hao,Wen Xie,Jie Yan,Liuluan Zhu

Cell reports 36:109674 PubMed34496236

2021

Tumor-initiating stem cell shapes its microenvironment into an immunosuppressive barrier and pro-tumorigenic niche.

Applications

Unspecified application

Species

Unspecified reactive species

Xi He,Sarah E Smith,Shiyuan Chen,Hua Li,Di Wu,Paloma I Meneses-Giles,Yongfu Wang,Mark Hembree,Kexi Yi,Xia Zhao,Fengli Guo,Jay R Unruh,Lucinda E Maddera,Zulin Yu,Allison Scott,Anoja Perera,Yan Wang,Chongbei Zhao,KyeongMin Bae,Andrew Box,Jeffrey S Haug,Fang Tao,Deqing Hu,Darrick M Hansen,Pengxu Qian,Subhrajit Saha,Dan Dixon,Shrikant Anant,Da Zhang,Edward H Lin,Weijing Sun,Leanne M Wiedemann,Linheng Li

Oxidative medicine and cellular longevity 2021:5529430 PubMed34326916

2021

Exosomal miR-218-5p/miR-363-3p from Endothelial Progenitor Cells Ameliorate Myocardial Infarction by Targeting the p53/JMY Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Ke,Rongfeng Yang,Fang Wu,Xing Wang,Jiawen Liang,Xun Hu,Chengheng Hu

Cancers 13: PubMed33808776

2021

EP4 as a Negative Prognostic Factor in Patients with Vulvar Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Buchholz,Aurelia Vattai,Sophie Fürst,Theresa Vilsmaier,Christina Kuhn,Elisa Schmoeckel,Doris Mayr,Christian Dannecker,Sven Mahner,Udo Jeschke,Helene H Heidegger

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PGE2 receptor EP4 subtype antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGE2 receptor EP4 subtype antibody (AB217966)

Western blot - Anti-PGE2 receptor EP4 subtype antibody (AB217966)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (5)

Product promise