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AB167424

Anti-PHKG2 antibody [EPR9727]

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(2 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal PHKG2 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

대체 명칭 보기

PHK-gamma-LT, PHK-gamma-T, PSK-C3, Phosphorylase kinase subunit gamma-2, PHKG2

4 이미지
Flow Cytometry (Intracellular) - Anti-PHKG2 antibody [EPR9727] (AB167424)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-PHKG2 antibody [EPR9727] (AB167424)

Intracellular flow cytometric analysis of permeabilized 293T cells labeling PHKG2 with ab167424 at 1/100 (red) or a rabbit IgG negative (green).

Immunocytochemistry/ Immunofluorescence - Anti-PHKG2 antibody [EPR9727] (AB167424)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PHKG2 antibody [EPR9727] (AB167424)

Immunofluorescent staining of HepG2 cells labeling PHKG2 with ab167424 at 1/100 dilution.

Western blot - Anti-PHKG2 antibody [EPR9727] (AB167424)
  • WB

Unknown

Western blot - Anti-PHKG2 antibody [EPR9727] (AB167424)

All lanes:

Western blot - Anti-PHKG2 antibody [EPR9727] (ab167424) at 1/1000 dilution

Lane 1:

Human fetal lung tissue lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

Ramos cell lysate at 10 µg

Lane 4:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Lane 5:

HeLa cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP antibody at 1/2000 dilution

Predicted band size: 46 kDa

false

Western blot - Anti-PHKG2 antibody [EPR9727] (AB167424)
  • WB

Lab

Western blot - Anti-PHKG2 antibody [EPR9727] (AB167424)

PHKG2 is a liver/testis isoform of the phosphorylase kinase gamma subunit enriched in testis (PMID : 8896567). Blocking and diluting buffer : 5% NFDM /TBST

Lanes 1 - 4:

Western blot - Anti-PHKG2 antibody [EPR9727] (ab167424) at 1/1000 dilution

Lanes 1 - 4:

Western blot - Anti-PHKG2 antibody [EPR9727] - BSA and Azide free (<a href='/ko/products/primary-antibodies/phkg2-antibody-epr9727-bsa-and-azide-free-ab249435'>ab249435</a>)

Lane 1:

Human testis tissue lysate at 20 µg

Lane 2:

Human lung tissue lysate at 20 µg

Lane 3:

Human brain tissue lysate at 20 µg

Lane 4:

Human breast tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 43 kDa

false

Exposure time: 80s

관련 conjugated 항체와 다양한 조성의 항체 (1)

  • Carrier free

    Anti-PHKG2 antibody [EPR9727] - BSA and Azide free

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9727

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Primary Antibodies

AB238006

Anti-Kappa light chain antibody [rKLC709]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa light chain antibody [rKLC709] (AB238006)

  • 0
  • 0
Primary Antibodies

AB167427

Anti-PNMT antibody [EPR7547(2)]

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-PNMT antibody [EPR7547(2)] (AB167427)

  • 0
  • 1
Primary Antibodies

AB9797

Anti-SDF1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDF1 antibody (AB9797)

  • 4
  • 14
Proteins & Peptides

AB89857

Recombinant human PHKG2 protein (GST tag N-Terminus)

Functional Studies - Recombinant human PHKG2 protein (AB89857)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100 - 1/500", "FlowCytIntra-species-notes": "<p><a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" } } }
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제품 세부 정보

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형
Liquid
보관 버퍼
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
배송 시 보관 조건
Conditional Ambient
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

PHKG2 also known as phosphorylase kinase gamma 2 is a catalytic subunit of the phosphorylase kinase (PhK) enzyme. PHKG2 has a molecular weight of approximately 44 kDa. This enzyme is responsible for the phosphorylation of serine residues in glycogen phosphorylase which is necessary for glycogen breakdown. PHKG2 is expressed mainly in muscle and liver tissues where it plays a role in the regulation of glycogen metabolism.
Biological function summary

Phosphorylase kinase involving PHKG2 is critical for the regulation of glycogenolysis. It forms a complex with other subunits including alpha beta and delta contributing to the full enzymatic activity of PhK. As part of this multi-subunit complex PHKG2 facilitates the conversion of glycogen to glucose-1-phosphate ensuring that energy production meets cellular demands during physical activity or fasting.

Pathways

The glycogenolysis process involving PHKG2 is tightly linked with the cAMP-dependent protein kinase (PKA) signaling pathway. Phosphorylase kinase gets activated by PKA under hormonal control prompting glycogen breakdown and maintaining glucose homeostasis. Additionally PHKG2's activity is connected with the insulin signaling pathway which integrates with its function to regulate carbohydrate metabolism especially relating to its interaction with proteins such as glycogen synthase and protein phosphatase 1.

Mutations in PHKG2 associate with Glycogen Storage Disease Type IX (GSD IX) a disorder characterized by an inability to properly break down glycogen leading to muscle weakness and hepatomegaly. PHKG2's interaction with proteins like alpha-glucosidase also links it to conditions like Pompe disease where glycogen buildup occurs due to deficient enzyme activity. Monitoring and understanding PHKG2's role in these contexts is important for developing therapeutic strategies.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Catalytic subunit of the phosphorylase b kinase (PHK), which mediates the neural and hormonal regulation of glycogen breakdown (glycogenolysis) by phosphorylating and thereby activating glycogen phosphorylase. May regulate glycogeneolysis in the testis. In vitro, phosphorylates PYGM (PubMed : 35549678).
See full target information PHKG2
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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Theranostics 11:5650-5674 PubMed33897873

2021

Endogenous glutamate determines ferroptosis sensitivity via ADCY10-dependent YAP suppression in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Zhang,Keke Yu,Lifang Ma,Zijun Qian,Xiaoting Tian,Yayou Miao,Yongjie Niu,Xin Xu,Susu Guo,Yueyue Yang,Zhixian Wang,Xiangfei Xue,Chuanjia Gu,Wentao Fang,Jiayuan Sun,Yongchun Yu,Jiayi Wang

Cell reports 34:108869 PubMed33730581

2021

The mitochondrial carrier SFXN1 is critical for complex III integrity and cellular metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Michelle Grace Acoba,Ebru S Selen Alpergin,Santosh Renuse,Lucía Fernández-Del-Río,Ya-Wen Lu,Oleh Khalimonchuk,Catherine F Clarke,Akhilesh Pandey,Michael J Wolfgang,Steven M Claypool
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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