AB272010

Anti-PHLPP2 antibody [SP220] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal PHLPP2 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.

대체 명칭 보기

KIAA0931, PHLPPL, PHLPP2, PH domain leucine-rich repeat-containing protein phosphatase 2, PH domain leucine-rich repeat-containing protein phosphatase-like, PHLPP-like

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHLPP2 antibody [SP220] - BSA and Azide free (AB272010)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHLPP2 antibody [SP220] - BSA and Azide free (AB272010)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human prostate carcinoma tissue sections labeling PHLPP2 with ab227673 at 1/100 dilution (1.65 μg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227673).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHLPP2 antibody [SP220] - BSA and Azide free (AB272010)

Formalin-fixed, paraffin-embedded human prostate tissue stained for PHLPP2 using ab227673 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227673).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHLPP2 antibody [SP220] - BSA and Azide free (AB272010)

Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for PHLPP2 using ab227673 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227673).

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP220

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>Primary antibody incubation for 1 hour at room temperature.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>Perform heat mediated antigen retrieval by boiling tissue section in citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes.Primary antibody incubation for 10 minutes at room temperature.</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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제품 세부 정보

ab272010 is the carrier-free version of ab227673.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A/G

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

Do Not Freeze

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

PHLPP2 also known as PH domain leucine-rich repeat protein phosphatase 2 functions as a serine/threonine phosphatase that specifically dephosphorylates Akt at the Ser473 site. This regulatory action on Akt influences many cellular processes including cell growth survival and metabolism. PHLPP2 has a molecular mass of approximately 140 kDa and is expressed in various tissues including liver and lung. Alternative names such as SCOP2 may sometimes reference this protein in specific research contexts.

Biological function summary

PHLPP2 modulates key signaling pathways by acting as a significant negative regulator of the Akt signaling pathway. It does not work alone; PHLPP2 can interact with functional complexes to exert its phosphatase activity more effectively. By dephosphorylating Akt PHLPP2 reduces the signaling output that leads to cell proliferation and survival responses leading to normal cellular homeostasis. This regulatory function positions PHLPP2 as a balancing component ensuring that Akt activity remains tightly controlled.

Pathways

PHLPP2 plays a role in the PI3K/Akt signaling pathway which is critical for cellular growth and metabolic functions. It also indirectly impacts the mTOR pathway through its regulation of Akt as Akt is an upstream activator of mTOR. By keeping Akt activity in check via dephosphorylation PHLPP2 keeps the downstream effects of the PI3K/Akt/mTOR axis in balance. It relates closely to proteins like PTEN which also acts as a negative regulator of the PI3K/Akt pathway ensuring that excessive Akt activity does not drive uncontrolled cellular events.

Mutations or altered expression of PHLPP2 have connections to cancer and diabetes. In cancer decreased levels of PHLPP2 can lead to an increase in Akt activity promoting uncontrolled cell division and survival therefore contributing to tumorigenesis. PHLPP2's influence on diabetes involves its regulation of insulin signaling where dysregulation can affect glucose homeostasis. The relationship between PHLPP2 Akt and insulin signaling highlights its important role in maintaining normal metabolic control and preventing disease progression in these contexts.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Protein phosphatase involved in regulation of Akt and PKC signaling. Mediates dephosphorylation in the C-terminal domain hydrophobic motif of members of the AGC Ser/Thr protein kinase family; specifically acts on 'Ser-473' of AKT1, 'Ser-660' of PRKCB isoform beta-II and 'Ser-657' of PRKCA. Akt regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of Akt triggers apoptosis and decreases cell proliferation. Also controls the phosphorylation of AKT3. Dephosphorylates STK4 on 'Thr-387' leading to STK4 activation and apoptosis (PubMed : 20513427). Dephosphorylates RPS6KB1 and is involved in regulation of cap-dependent translation (PubMed : 21986499). Inhibits cancer cell proliferation and may act as a tumor suppressor. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. Dephosphorylates RAF1 inhibiting its kinase activity (PubMed : 24530606).

See full target information PHLPP2

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PHLPP2 antibody [SP220] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHLPP2 antibody [SP220] - BSA and Azide free (AB272010)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHLPP2 antibody [SP220] - BSA and Azide free (AB272010)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHLPP2 antibody [SP220] - BSA and Azide free (AB272010)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

Product promise