AB41433

Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [M156]

Name: Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [M156]
Brand: Abcam Limited
SKU: AB41433
Rating: 5 (3 reviews)

KO Validated
각 태그의 뜻

(3 리뷰들)

(8 제품이 사용된 논문 )

Mouse Monoclonal Phospholipase C gamma 1/PLC-gamma-1 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Mouse, Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human PLCG1.

2 이미지

Flow Cytometry (Intracellular) - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [M156] (AB41433)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [M156] (AB41433)

Overlay histogram showing Jurkat cells stained with ab41433 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab41433, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Lab

Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [M156] (AB41433)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : Phospholipase C gamma 1/PLC-gamma-1 knockout HAP1 cell lysate (20 μg)
Lane 3 : HepG2 cell lysate (20 μg)
Lane 4 : Mouse brain tissue lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab41433 observed at 160 kDa. Red - loading control, ab181602, observed at 37 kDa.

ab41433 was shown to recognize Phospholipase C gamma 1/PLC-gamma-1 when Phospholipase C gamma 1/PLC-gamma-1 knockout samples were used, along with additional cross-reactive bands. Wild-type and Phospholipase C gamma 1/PLC-gamma-1 knockout samples were subjected to SDS-PAGE. ab41433 diluted to 1/1000 and ab181602 (loading control to GAPDH) diluted to 1/10,000 and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye^® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye^® 680RD) preadsorbed (ab216777) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [M156] (ab41433)

Predicted band size: 148 kDa

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주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

M156

Isotype

IgG1

Carrier free

Reacts with

Mouse, Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

Synthetic Peptide within Human PLCG1. The exact immunogen used to generate this antibody is proprietary information.

P19174

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "<a href='/ko/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

보관 정보

Stable for 12 months at -20°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Phospholipase C gamma 1 (PLC-gamma-1) also known as PLCG1 or PLC-gamma plays an important role in cellular signaling. This enzyme has a molecular mass of approximately 150 kDa. It is found in multiple cell types but is highly expressed in the brain and immune cells. PLC-gamma-1 is involved in the hydrolysis of phosphatidylinositol 45-bisphosphate (PIP2) to generate inositol 145-trisphosphate (IP3) and diacylglycerol (DAG) leading to downstream signaling events that control various cellular processes.

Biological function summary

PLC-gamma-1 is integral in signal transduction and is an important player in the immune response. The protein does not function as part of a large multi-protein complex but interacts transiently with other signaling molecules. It acts downstream of growth factor receptors and immunoreceptors by mediating calcium release and activation of protein kinase C influencing cell proliferation migration and differentiation. Its expression pattern suggests a critical role in the nervous and immune systems affecting learning memory and immune defense.

Pathways

PLC-gamma-1 is an important component in both the phospholipase C and PLC-gamma pathways. These pathways include important interactions with proteins such as the receptor tyrosine kinases and Src family kinases. In these pathways PLC-gamma-1 modulates signals that guide cellular responses to external stimuli affecting processes like growth signaling and T-cell receptor activation which is vital in adaptive immunity.

PLC-gamma-1 has significant connections to cancer and immune deficiencies. Abnormal PLC-gamma-1 activity is observed in certain cancers such as breast cancer where it influences tumor growth and metastasis. Its dysregulation in immune cells leads to disorders like autoimmune diseases by impacting proteins such as LAT and SLP-76 which are important in T-cell signaling. Understanding PLC-gamma-1’s regulatory mechanisms helps in developing targeted therapies for these conditions.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Mediates the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). Plays an important role in the regulation of intracellular signaling cascades. Becomes activated in response to ligand-mediated activation of receptor-type tyrosine kinases, such as PDGFRA, PDGFRB, EGFR, FGFR1, FGFR2, FGFR3 and FGFR4 (By similarity). Plays a role in actin reorganization and cell migration (PubMed : 17229814). Guanine nucleotide exchange factor that binds the GTPase DNM1 and catalyzes the dissociation of GDP, allowing a GTP molecule to bind in its place, therefore enhancing DNM1-dependent endocytosis (By similarity).

See full target information PLCG1

대체 명칭 보기

PLC1, PLCG1, PLC-148, Phosphoinositide phospholipase C-gamma-1, Phospholipase C-II, Phospholipase C-gamma-1, PLC-II, PLC-gamma-1

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제품이 사용된 논문 (8)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Mediators of inflammation 2025:3096848 PubMed40034562

2025

Taohong Siwu Decoction Combined With the LncRNA H19/miR-675-5p Axis Repairs Limb Ischemia-Reperfusion Injury Through the Regulation of the Wnt3a/Ca Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Fuping Zhu,Hui Liu,Yinsheng Cao,Bing Dai,Hang Wu,Yutong Zhu,Wuping Li

Cells 13: PubMed39682716

2024

Sprouty2 Regulates Endocytosis and Degradation of Fibroblast Growth Factor Receptor 1 in Glioblastoma Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Barbara Hausott,Lena Pircher,Michaela Kind,Jong-Whi Park,Peter Claus,Petra Obexer,Lars Klimaschewski

Environmental toxicology 39:357-366 PubMed37755144

2023

MiRNA-26a-5p inhibits preterm labor initiation by targeting and regulating TRPC3 ion channel protein expression.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Chen,Tong Liu,Hong Cui,Quan Na,Sishi Liu

Oxidative medicine and cellular longevity 2021:8542809 PubMed34650665

2021

Dihydromyricetin Ameliorates Inflammation-Induced Insulin Resistance via Phospholipase C-CaMKK-AMPK Signal Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Lianjie Hou,Fangyi Jiang,Bo Huang,Weijie Zheng,Yufei Jiang,Gengyuan Cai,Dewu Liu,Ching Yuan Hu,Chong Wang

The Journal of clinical investigation 130:3087-3097 PubMed32069268

2020

Single residue in CD28-costimulated CAR-T cells limits long-term persistence and antitumor durability.

Applications

Unspecified application

Species

Unspecified reactive species

Sonia Guedan,Aviv Madar,Victoria Casado-Medrano,Carolyn Shaw,Anna Wing,Fang Liu,Regina M Young,Carl H June,Avery D Posey

Cell stem cell 23:181-192.e5 PubMed30082067

2018

Human iPSC-Derived Natural Killer Cells Engineered with Chimeric Antigen Receptors Enhance Anti-tumor Activity.

Applications

Unspecified application

Species

Unspecified reactive species

Ye Li,David L Hermanson,Branden S Moriarity,Dan S Kaufman

The Journal of biological chemistry 286:15268-77 PubMed21245141

2011

Up-regulation of AMP-activated protein kinase in cancer cell lines is mediated through c-Src activation.

Applications

Species

Human

Sarit Mizrachy-Schwartz,Noam Cohen,Shoshana Klein,Nataly Kravchenko-Balasha,Alexander Levitzki

BMB reports 42:516-22 PubMed19712589

2009

SH2D4A regulates cell proliferation via the ERalpha/PLC-gamma/PKC pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Tingting Li,Wei Li,Jingyu Lu,Hong Liu,Yinghui Li,Yanyan Zhao

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [M156]

Flow Cytometry (Intracellular) - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [M156] (AB41433)

Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [M156] (AB41433)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (8)

Product promise