AB233238

Anti-Phospholipase D antibody

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(2 제품이 사용된 논문 )

Rabbit Polyclonal PLD1 antibody. Suitable for WB, IHC-P and reacts with Pig, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PLD1 aa 700 to C-terminus.

대체 명칭 보기

Phospholipase D1, PLD 1, hPLD1, Choline phosphatase 1, Phosphatidylcholine-hydrolyzing phospholipase D1, PLD1

8 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase D antibody (AB233238)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase D antibody (AB233238)

Formalin-fixed, paraffin-embedded human cerebrum tissue stained for PLD1 using ab233238 at 10 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase D antibody (AB233238)

Formalin-fixed, paraffin-embedded human kidney tissue stained for PLD1 using ab233238 at 10 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase D antibody (AB233238)

Formalin-fixed, paraffin-embedded human glioma tissue stained for PLD1 using ab233238 at 10 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase D antibody (AB233238)

Formalin-fixed, paraffin-embedded human stomach tissue stained for PLD1 using ab233238 at 10 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase D antibody (AB233238)

Formalin-fixed, paraffin-embedded human liver tissue stained for PLD1 using ab233238 at 10 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase D antibody (AB233238)

Formalin-fixed, paraffin-embedded human pancreatic cancer tissue stained for PLD1 using ab233238 at 10 μg/ml in immunohistochemical analysis. DAB staining.

Supplier Data

Western blot - Anti-Phospholipase D antibody (AB233238)

All lanes:

Western blot - Anti-Phospholipase D antibody (ab233238) at 1 µg/mL

All lanes:

Pig small intestine tissue

Secondary

All lanes:

HRP-Linked Guinea pig Anti-Rabbit Ab at 1/2000 dilution

Predicted band size: 124 kDa

false

Supplier Data

Western blot - Anti-Phospholipase D antibody (AB233238)

All lanes:

Western blot - Anti-Phospholipase D antibody (ab233238) at 1 µg/mL

All lanes:

Recombinant human PLD1

Secondary

All lanes:

HRP-Linked Guinea pig Anti-Rabbit Ab at 1/2000 dilution

Predicted band size: 124 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Pig

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human PLD1 aa 700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q13393

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-5 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chinese hamster": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-5 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

Antigen-specific affinity chromatography followed by Protein A affinity chromatography.

보관 버퍼

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Phospholipase D (PLD) is an enzyme involved in the hydrolysis of phospholipids particularly phosphatidylcholine into phosphatidic acid and choline. This enzymatic reaction is important for signal transduction and membrane trafficking. In humans PLD primarily consists of two isoforms PLD1 and PLD2 each with a molecular mass of approximately 120-130 kDa. They are mainly expressed in the cellular membrane but also found in the cytosol and various intracellular organelles. These isoforms differ in their regulatory mechanisms and tissue distribution providing them specialized functions within the cell.

Biological function summary

Phospholipase D plays roles in cellular processes such as cell proliferation differentiation and survival. The phospholipase D activity results in the production of phosphatidic acid a lipid mediator involved in vesicle trafficking and actin cytoskeleton remodeling. PLD functions within a protein complex with small GTP-binding proteins like ARF and Rho family members enhancing its catalytic activity and facilitating its involvement in complex cellular responses.

Pathways

Phospholipase D participates in the mTOR signaling pathway and the PI3K-Akt pathway. These pathways are key to regulating cell growth metabolism and survival. PLD-generated phosphatidic acid acts as a signaling lipid that activates mTOR complex 1 a central component in the mTOR signaling pathway. Additionally phospholipase D interacts with proteins like PI4P5 kinase in the PI3K-Akt pathway linking it to phosphoinositide metabolism and indicating its integrative role in signaling networks.

Phospholipase D connects to cancer and neurodegenerative diseases. The overexpression and altered activity of PLD have been observed in several types of cancer particularly breast cancer where it is thought to contribute to tumor growth and metastasis. In Alzheimer's disease dysregulated PLD activity may be associated with abnormal phosphatidic acid levels impacting neural cell membrane integrity and signaling. In these contexts phospholipase D interacts with proteins such as amyloid precursor protein in Alzheimer's highlighting the broader implications of PLD in disease pathogenesis and potential therapeutic targeting.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Function as phospholipase selective for phosphatidylcholine (PubMed : 25936805, PubMed : 8530346, PubMed : 9582313). Implicated as a critical step in numerous cellular pathways, including signal transduction, membrane trafficking, and the regulation of mitosis. May be involved in the regulation of perinuclear intravesicular membrane traffic (By similarity).

See full target information PLD1

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Evidence-based complementary and alternative medicine : eCAM 2022:1562219 PubMed35836822

2022

Suppression of Long Noncoding RNA SNHG1 Inhibits the Development of Hypopharyngeal Squamous Cell Carcinoma via Increasing PARP6 Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Chen,Xiao He,Bin Li,Jingjing Chen,Xuxia Tang

Journal of cellular physiology 235:6592-6604 PubMed32067228

2020

Promotion of cell autophagy and apoptosis in cervical cancer by inhibition of long noncoding RNA LINC00511 via transcription factor RXRA-regulated PLD1.

Applications

Unspecified application

Species

Unspecified reactive species

Yangyang Shi,Mengran Liu,Yan Huang,Jing Zhang,Ling Yin

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Phospholipase D antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase D antibody (AB233238)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase D antibody (AB233238)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase D antibody (AB233238)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase D antibody (AB233238)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase D antibody (AB233238)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase D antibody (AB233238)

Western blot - Anti-Phospholipase D antibody (AB233238)

Western blot - Anti-Phospholipase D antibody (AB233238)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Assay Kits

Secondary Antibodies

Reagents, Controls & Accessories

Secondary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (2)

Product promise