AB242095

Anti-Phosphomevalonate kinase antibody

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Rabbit Polyclonal Phosphomevalonate kinase antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human PMVK aa 100 to C-terminus.

대체 명칭 보기

PMKI, PMVK, Phosphomevalonate kinase, PMKase, hPMK

2 이미지

Supplier Data

Immunoprecipitation - Anti-Phosphomevalonate kinase antibody (AB242095)

Phosphomevalonate kinase was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab242095 at 8 μL/reaction. Western blot was performed from the immunoprecipitate using ab242095 at 1/1000 dilution.

Lane 1 : ab242095 IP in HEK-293T whole cell lysate.
Lane 2 : Control IgG IP in HEK-293T whole cell lysate.

Detection : Chemiluminescence with exposure time of 3 seconds.

Lysates prepared in NETN buffer.

All lanes:

Immunoprecipitation - Anti-Phosphomevalonate kinase antibody (ab242095)

Predicted band size: 21 kDa

false

Supplier Data

Western blot - Anti-Phosphomevalonate kinase antibody (AB242095)

Lysates prepared in NETN buffer.

All lanes:

Western blot - Anti-Phosphomevalonate kinase antibody (ab242095) at 1/1000 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 4:

TCMK-1 (mouse kidney epithelial cell line) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 21 kDa

true

Exposure time: 3s

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human PMVK aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q15126

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "8 - 15 μl/mg lysate", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

Antibody was affinity purified using an epitope specific to Phosphomevalonate kinase immobilized on solid support.

보관 버퍼

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Phosphomevalonate kinase (PMVK) is an enzyme involved in the mevalonate pathway a critical metabolic pathway that produces isoprenoids. Known also as mevalonate-5-phosphate kinase this enzyme catalyzes the ATP-dependent phosphorylation of mevalonate-5-phosphate to mevalonate-5-diphosphate. The molecular weight of PMVK is approximately 21 kDa. PMVK is expressed in various tissues with significant expression in the liver and adrenal glands reflecting its role in the biosynthesis of cholesterol and other isoprenoids.

Biological function summary

Phosphomevalonate kinase functions as an important regulatory enzyme in the synthesis of cholesterol and non-sterol isoprenoids. It does not operate as part of a larger protein complex but through its sole catalytic activity it impacts the production of downstream molecules essential for cell membrane integrity and steroid hormone production. The enzyme's activity can affect the availability of isoprenoid precursors linking metabolic flux through the mevalonate pathway to cellular growth and differentiation processes.

Pathways

Phosphomevalonate kinase plays a central role in the mevalonate pathway a vital metabolic pathway for cholesterol biosynthesis. It is responsible for converting mevalonate-5-phosphate to mevalonate-5-diphosphate a necessary step towards producing farnesyl pyrophosphate. This pathway is closely related to the biosynthesis of cholesterol and other essential isoprenoids. PMVK activity is associated with other enzymes in the pathway like HMG-CoA reductase a major control point in cholesterol biosynthesis.

Phosphomevalonate kinase's role in the mevalonate pathway relates to disorders involving cholesterol metabolism such as hypercholesterolemia and mevalonic aciduria. Mutations or misregulation of phosphomevalonate kinase can lead to altered cholesterol synthesis impacting cardiovascular health. Moreover PMVK may interact indirectly with proteins like LDL-receptor further linking its function to the regulation of cholesterol levels and associated diseases.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Catalyzes the reversible ATP-dependent phosphorylation of mevalonate 5-phosphate to produce mevalonate diphosphate and ADP, a key step in the mevalonic acid mediated biosynthesis of isopentenyl diphosphate and other polyisoprenoid metabolites.

See full target information PMVK

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Experimental and therapeutic medicine 23:3 PubMed34815755

2021

Hirudin, a thrombin inhibitor, attenuates TGF-β-induced fibrosis in renal proximal tubular epithelial cells by inhibition of protease-activated receptor 1 expression via S1P/S1PR2/S1PR3 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Qiang Lin,Chunli Long,Zhengang Wang,Ronghui Wang,Wei Shi,Jiwei Qiu,Junlin Mo,Yongxiang Xie

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Phosphomevalonate kinase antibody

Immunoprecipitation - Anti-Phosphomevalonate kinase antibody (AB242095)

Western blot - Anti-Phosphomevalonate kinase antibody (AB242095)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Proteins & Peptides

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise