Anti-Phosphoserine antibody

• WB

AbReview19643****

Western blot - Anti-Phosphoserine antibody (AB9332)

Blocking Step : 10% Milk for 1 hour at room temperature

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Western blot - Anti-Phosphoserine antibody (ab9332) at 3 µg/mL

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Whole cell lysate of monkey COS7 cells at 25 µg

Secondary

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An HRP-conjugated Pig anti-rabbit IgG polyclonal at 1/3000 dilution

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This image is courtesy of an Abreview submitted by Karin Birkenkamp-Demtroeder

• WB

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Western blot - Anti-Phosphoserine antibody (AB9332)

Western blotting of a melanoma cell lysate with anti-phosphoserine antibodies

The detected band is 53 kD, and is a similar size to the p53 tumor suppressor factor.

The cells were treated with 0, 50, 200 or 400 J UV (lane A to D, respectively) and with 0.1uM of okadaic acid(lane E). Actin level was measured as an internal standard of cell protein.

Western blotting of a melanoma cell lysate with anti-phosphoserine antibodies. The detected band is 53 kD, and is a similar size to the p53 tumor suppressor factor. The cells were treated with 0, 50, 200 or 400 J UV (lane A to D, respectively) and with 0.1uM of okadaic acid (lane E). Actin level was measured as an internal standard of cell protein.

Lane 1:

Western blot - Biotin Anti-Phosphoserine antibody (<a href='/ko/products/primary-antibodies/biotin-phosphoserine-antibody-ab9335'>ab9335</a>)

Lane 1:

Western blot - Anti-Phosphoserine antibody (ab9332)

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• WB

CiteAb

Western blot - Anti-Phosphoserine antibody (AB9332)

Phosphoserine western blotting using Anti-Phosphoserine antibody ab9332. Publication image and figure legend from Pei, C. J., He, Q. X., et al., 2022, Acta Biochim Biophys Sin (Shanghai), Pubmed 35866601.

Figure 1AtMKK5 effectively phosphorylates AtMPK6 at both Thr and Tyr sites(A) Schematic diagram of AtMKK5. The structural elements are colored as follows : KIM (grey), kinase domain (N-lobe, yellow; C-lobe, light blue; activation segment, pink). (B) Phosphorylation states of AtMKK5 analyzed using anti-pSer and anti-pThr antibodies. (C) Time courses of AtMKK5-catalyzed ATP hydrolysis and AtMPK6 phosphorylation. The additions of p-AtMKK5 (20 nM) and AtMPK6(K92M) (3.0 μm) to the reaction mixture (containing 1 mM ATP) are indicated. (D) Time course of AtMPK6 phosphorylation after subtracting AtMKK5-catalyzed ATP hydrolysis. (E) Phosphorylation states of AtMPK6 analyzed using anti-pThr, anti-pTyr and anti-ERK2/pTpY antibodies. (F-G) Dependence of the initial rate of AtMKK5-catalyzed reaction on the concentration of ATP (F) and AtMPK6(K92M) (G). The reaction mixture contained 20 nM p-AtMKK5 in standard assay buffer, and K m for AtMPK6 was measured at 1 mM ATP. The solid lines are the best fitting result according to the Michaelis-Menten equation.

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