- ICC/IF
Unknown
Immunocytochemistry/ Immunofluorescence - Anti-Phosphotyrosine antibody [IG2] (AB5592)
Immunolocalization of phosphotyrosine in HeLa cells using ab5592.
- ELISA
Supplier Data
ELISA - Anti-Phosphotyrosine antibody [IG2] (AB5592)
Direct ELISA of a 96 well plate coated with 100 uL per well of phosphotyrosine, phosphoserine or phosphothreonine at 5 μg/mL, incubated with 100 μL per well of ab5592
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This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Phosphorylated tyrosine residues modify the function and activity of proteins within cells. It is a critical component of signal transduction pathways and can alter protein functions when becoming part of large protein complexes. It often regulates receptor proteins and intracellular kinases modifying their ability to interact with other proteins like SH2 and PTB domain-containing proteins. Phosphotyrosine antibodies like anti-phosphotyrosine Ig2 are helpful research tools for detecting these modifications.
Pathways
The modification of tyrosine to phosphotyrosine participates significantly in pathways such as the MAPK/ERK pathway and the PI3K/AKT pathway. These pathways are important for various cellular processes including growth and survival. Proteins like EGFR and PDGFR regulate signaling cascades by phosphorylation of tyrosine residues and often interact with phosphotyrosine and phosphotyrosine antibodies importantly.
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