AB269515

Anti-Phosphotyrosine antibody [Py72]

Recombinant
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Mouse Recombinant Monoclonal Phosphotyrosine antibody. Suitable for IP, WB and reacts with Modified Amino Acid samples.

대체 명칭 보기

L-Phosphotyrosine, L-Tyrosine-O-phosphate, O-Phospho-L-tyrosine, O-Phosphotyrosine, Phospho-L-tyrosine, Phosphonotyrosine

3 이미지

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Immunoprecipitation - Anti-Phosphotyrosine antibody [Py72] (AB269515)

Phosphotyrosine was immunoprecipitated from MCF7 (human breast adenocarcinoma epithelial cell), treated with 1mM pervanadate for 20 minutes, whole cell lysates, with ab269515 at 1/30 dilution (2μg in 0.35mg lysates, (32.667μg/ml)). Western blot was performed on the immunoprecipitate using ab269515 at 1/1000 dilution (0.196μg/ml). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : MCF7 treated with 1mM pervanadate for 20 mins, whole cell lysates, 10μg.

Lane 2 : ab269515 IP in MCF7 treated with 1mM pervanadate for 20 mins, whole cell lysates.

Lane 3 : Mouse monoclonal IgG1 (ab18443) instead of ab269515 in MCF7 treated with 1mM pervanadate for 20 mins, whole cell lysates.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 1 second.

Multiple bands represent phosph-tyrosine containing proteins precipitated and detected by ab269515.

All lanes:

Immunoprecipitation - Anti-Phosphotyrosine antibody [Py72] (ab269515)

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Immunoprecipitation - Anti-Phosphotyrosine antibody [Py72] (AB269515)

Phosphotyrosine was immunoprecipitated from NIH/3T3 (Mouse embyro fibroblast cells), treated with 10mM pervanadate for 20 minutes, whole cell lysates, with ab269515 at 1/30 dilution (2μg in 0.35mg lysates, (32.667μg/ml)). Western blot was performed on the immunoprecipitate using ab269515 at 1/1000 dilution (0.196μg/ml). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : NIH/3T3 treated with 10mM pervanadate for 20 mins, whole cell lysates, 10μg.

Lane 2 : ab269515 IP in NIH/3T3 treated with 10mM pervanadate for 20 mins, whole cell lysates.

Lane 3 : Mouse monoclonal IgG1 (ab18443) instead of ab269515 in NIH/3T3 treated with 10mM pervanadate for 20 mins, whole cell lysates.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 1 second.

Multiple bands represent phosph-tyrosine containing proteins precipitated and detected by ab269515.

All lanes:

Immunoprecipitation - Anti-Phosphotyrosine antibody [Py72] (ab269515)

false

Lab

Western blot - Anti-Phosphotyrosine antibody [Py72] (AB269515)

Multiple bands represent phosph-tyrosine containing proteins detected by ab269515.

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure times : Lanes 1-4 : 3.25 secs; Lanes 5-6 : 3 mins.

All lanes:

Western blot - Anti-Phosphotyrosine antibody [Py72] (ab269515) at 0.196 µg/mL

Lane 1:

NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 20 µg

Lane 2:

NIH/3T3 treated with 10mM pervanadate for 20 minutes, whole cell lysates at 20 µg

Lane 3:

Daudi (human Burkitt's lymphoma lymphoblast), whole cell lysate at 20 µg

Lane 4:

Daudi treated with 10mM pervanadate for 30 minutes, whole cell lysates at 20 µg

Lane 5:

MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 6:

MCF7 treated with 1mM pervanadate for 20 minutes, whole cell lysates at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/100000 dilution

false

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

Py72

Isotype

IgG1

Carrier free

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Modified Amino Acid": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "32.667 µg/mL", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.196 µg/mL", "WB-species-notes": "<p></p>" } } }

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제품 세부 정보

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Phosphotyrosine also known simply as pY is a post-translational modification where a phosphate group binds to the hydroxyl group of a tyrosine amino acid in proteins. This small modification can have a huge impact on proteins' function and interaction. Phosphotyrosine has a molecular weight of approximately 216 Da when it stands on its own. This modification can occur in various proteins expressed widely across tissues playing a role in signaling cell division and morphological changes.

Biological function summary

Phosphorylated tyrosine residues modify the function and activity of proteins within cells. It is a critical component of signal transduction pathways and can alter protein functions when becoming part of large protein complexes. It often regulates receptor proteins and intracellular kinases modifying their ability to interact with other proteins like SH2 and PTB domain-containing proteins. Phosphotyrosine antibodies like anti-phosphotyrosine Ig2 are helpful research tools for detecting these modifications.

Pathways

The modification of tyrosine to phosphotyrosine participates significantly in pathways such as the MAPK/ERK pathway and the PI3K/AKT pathway. These pathways are important for various cellular processes including growth and survival. Proteins like EGFR and PDGFR regulate signaling cascades by phosphorylation of tyrosine residues and often interact with phosphotyrosine and phosphotyrosine antibodies importantly.

The dysregulation of phosphotyrosine levels contributes to conditions like cancer and diabetes. Aberrant activation or overexpression of phosphotyrosine residues on receptors like HER2 and insulin receptors can promote uncontrolled cell proliferation often seen in tumors and contribute to insulin resistance respectively. As such phosphotyrosine serves not only as a biological target but also as a potential therapeutic target in the treatment of these disorders.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

See full target information Phosphotyrosine

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Phosphotyrosine antibody [Py72]

Immunoprecipitation - Anti-Phosphotyrosine antibody [Py72] (AB269515)

Immunoprecipitation - Anti-Phosphotyrosine antibody [Py72] (AB269515)

Western blot - Anti-Phosphotyrosine antibody [Py72] (AB269515)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Applications

Immunogen

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

Product promise